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Small polymer enzyme-antibody fragment as well as preparation and application thereof

An antibody fragment and multimeric enzyme technology, applied in the field of medical detection, can solve the problems affecting the polymer efficiency, increase the molecular weight, occupy a large space, etc., to reduce the production of enzyme-labeled conjugates, improve the effectiveness, Small footprint effect

Pending Publication Date: 2021-02-02
东莞市医本生物科技有限公司
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Problems solved by technology

However, the disadvantages of this method are: the molecular weight is significantly increased, and the space occupied is too large. Due to steric hindrance and other reasons, it affects the efficiency of polymers entering the interior of tissue cells and binding specific targets, especially for some relatively small cells or subcellular structures. The detection of internal antigens, the impact is more obvious

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  • Small polymer enzyme-antibody fragment as well as preparation and application thereof
  • Small polymer enzyme-antibody fragment as well as preparation and application thereof
  • Small polymer enzyme-antibody fragment as well as preparation and application thereof

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0040] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0041] see Figure 1-Figure 7, a small polymer enzyme-antibody fragment, which is obtained by polymerizing an activated enzyme with a first-generation polyamide-amine dendrimer (G1-PAMAM) and conjugated with a sulfhydryl-modified antibody fragment A small polymerase-linked secondary antibody;

[0042] in:

[0043] The polymerase complex is prepared by coupling the labeled enzyme with a first-generation polyamide-amine dendrimer (G1-PAMAM) after oxidation by sodium perioda...

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Abstract

The invention provides a small multimeric enzyme-antibody fragment as well as preparation and an application thereof. The preparation method is characterized by polymerizing sodium periodate oxidizedand activated enzyme and a PAMAM dendritic polymer occupying a small space to obtain a small multimeric enzyme (Micro-Polymer); in order to further reduce the space occupation of a conjugate, an antibody to be coupled is treated to prepare the antibody fragment (semi-antibody, rIgG) with a specific connection site, so that the mass and the volume of the antibody are reduced by half; and finally, the small multimeric enzyme antibody fragment with smaller space occupation is obtained by directionally connecting the small multimeric enzyme and the antibody fragment through a heterobifunctional crosslinker.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a small polymer enzyme-antibody fragment and its preparation and application. Background technique [0002] Immunohistochemistry (IHC) technology uses the principle of specific binding of antigen and antibody to detect the color of the chromogenic substance (fluorescein, enzyme, metal ion, isotope) labeled on the antibody through chemical reaction to detect the tissue cells. Antigen, technology for its localization, qualitative and quantitative research. [0003] Such as figure 1 As shown, the markers of the initial immunohistochemical secondary antibodies were mostly single enzymes or fluorescein and other markers, because the number of enzymes or other markers (enzymes are most commonly used as markers) attached to the secondary antibody was small, Therefore, the detection sensitivity is very low, and a high primary antibody concentration is required to obtain a pos...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/535G01N33/574G01N33/554G01N33/543
CPCG01N33/535G01N33/54393G01N33/554G01N33/574G01N33/581
Inventor 张海华
Owner 东莞市医本生物科技有限公司
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