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SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as method and application of SNP molecular marker

A molecular marker, Scutellaria baicalensis technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of low technology scalability and low accuracy

Active Publication Date: 2021-02-05
CHINA TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the identification of origin of Scutellaria baicalensis mostly uses methods such as character identification and microscopic identification, relying on personal experience and subjective judgment, the accuracy is not high, and the technology is not scalable.

Method used

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  • SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as method and application of SNP molecular marker
  • SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as method and application of SNP molecular marker
  • SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as method and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Sample information: Inner Mongolia (HQ361, HQ362, HQ401, HQ402, HQ431, HQ432), Liaoning-Jilin (HQ481, HQ482, HQ501, HQ502, HQ541, HQ542), Shaanxi-Shanxi-Shandong (HQ351, HQ381, HQ382, HQ391) , HQ392, HQ471, HQ472, HQ491, HQ492) area of ​​Scutellaria baicalensis. The specific information of the samples can be found in Table 2.

[0063] Identification steps:

[0064] 1. Sample DNA extraction

[0065] The mCTAB method (modified CTAB method) was used to extract DNA from the leaves of Scutellaria baicalensis in each sample. The specific extraction steps are as follows:

[0066] (1) Put 100 mg of fresh plant leaves into a 2.0 mL centrifuge tube, add a small amount of quartz sand and a magnetic bead at the same time, immerse the fresh leaves in liquid nitrogen for about 1 min, and grind them in a grinder until they are fine. powder ready for use;

[0067] (2) Add 1.5 mL buffer A (0.1 M Tris-HCl pH 8.0; 5 mMEDTA; 0.25 M NaCl; 1 % PVP-40) solution to the ground material, an...

Embodiment 2

[0091] Sample information: Scutellaria baicalensis were collected from Inner Mongolia (HQ363), Liaoning-Jilin (HQ483, HQ503), Shaanxi-Shanxi-Shandong (HQ383, HQ393, HQ493). The specific information of the samples can be found in Table 2.

[0092] Table 2 Specific information of samples

[0093]

[0094] The SNP sites in Table 1 in Example 1 were used to identify Scutellaria baicalensis in Inner Mongolia area and Scutellaria baicalensis in other regions. Refer to the SNP site information acquisition method in Example 1 to obtain the SNP site information of each sample at the same position.

[0095] The SNP genes at the same positions in each sample were compared with the SNP genes of Scutellaria baicalensis in Inner Mongolia in Table 1, and the comparison results are shown in Table 3.

[0096] Table 3 SNP gene identification results

[0097]

[0098] Note: M stands for CA heterozygote. A single A or C or G or T represents homozygosity.

[0099] From the identification...

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Abstract

The invention relates to a SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as a method and application of the SNP molecular marker. The molecular markercomprises 19 SNP molecular marker bodies, and the nucleotide sequences of the 19 SNP molecular marker bodies are shown as SEQ ID NO.1 - SEQ ID NO.19. According to the SNP molecular marker for identifying the scutellaria baicalensis produced in the Inner Mongolia as well as the method and the application of the SNP molecular marker, the genomics technology is adopted, the SNP marker is obtained onthe genome level of the scutellaria baicalensis, and whether a detected scutellaria baicalensis is an Inner Mongolia producing area or not can be rapidly and accurately determined.

Description

technical field [0001] The invention belongs to the field of molecular biology molecular markers, and particularly relates to a SNP molecular marker for identifying Scutellaria baicalensis produced in Inner Mongolia and its method and application. Background technique [0002] Scutellaria baicalensis is a widely used traditional Chinese medicinal material. Its root is used as medicine. It is bitter in taste and cold in nature. Indications for febrile disease, upper respiratory tract infection, lung-heat cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle, swollen boils and other diseases. The clinical antibacterial activity of Scutellaria baicalensis is better than that of Coptis chinensis, and it does not produce drug resistance. According to relevant statistics, 70% of Chinese patent medicines currently on the market contain Scutellaria baicalensis, such as commonly used Chinese patent medicines Qingkailing...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6895C12Q2600/156C12Q2535/122C12Q2537/165
Inventor 王继永郑司浩曾燕刘美娟尚兴朴赵莎王浩刘国城静一孙文静朱亚兵
Owner CHINA TRADITIONAL CHINESE MEDICINE
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