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A SNP molecular marker for identifying Scutellaria baicalensis produced in Inner Mongolia and its method and application
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A technology of molecular markers and Scutellaria baicalensis, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of poor technology scalability and low accuracy
Active Publication Date: 2021-04-20
CHINA TRADITIONAL CHINESE MEDICINE +1
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At present, the identification of origin of Scutellaria baicalensis mostly uses methods such as character identification and microscopic identification, relying on personal experience and subjective judgment, the accuracy is not high, and the technology is not scalable.
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Embodiment 1
[0062] Sample information: Inner Mongolia (HQ361, HQ362, HQ401, HQ402, HQ431, HQ432), Liaoning-Jilin (HQ481, HQ482, HQ501, HQ502, HQ541, HQ542), Shaanxi-Shanxi-Shandong (HQ351, HQ381, HQ382, HQ391) , HQ392, HQ471, HQ472, HQ491, HQ492) area of Scutellaria baicalensis. The specific information of the samples can be found in Table 2.
[0065] The mCTAB method (modified CTAB method) was used to extract DNA from the leaves of Scutellaria baicalensis in each sample. The specific extraction steps are as follows:
[0066] (1) Put 100 mg of fresh plant leaves into a 2.0 mL centrifuge tube, add a small amount of quartz sand and a magnetic bead at the same time, immerse the fresh leaves in liquid nitrogen for about 1 min, and grind them in a grinder until they are fine. powder ready for use;
[0067] (2) Add 1.5 mL buffer A (0.1 M Tris-HCl pH 8.0; 5 mMEDTA; 0.25 M NaCl; 1 % PVP-40) solution to the ground material, an...
Embodiment 2
[0091] Sample information: Scutellaria baicalensis were collected from Inner Mongolia (HQ363), Liaoning-Jilin (HQ483, HQ503), Shaanxi-Shanxi-Shandong (HQ383, HQ393, HQ493). The specific information of the samples can be found in Table 2.
[0092] Table 2 Specific information of samples
[0093]
[0094] The SNP sites in Table 1 in Example 1 were used to identify Scutellaria baicalensis in the Inner Mongolia area and Scutellaria baicalensis in other regions. Refer to the SNP site information acquisition method in Example 1 to obtain the SNP site information of each sample at the same position.
[0095] The SNP genes at the same positions in each sample were compared with the SNP genes of Scutellaria baicalensis in Inner Mongolia in Table 1, and the comparison results are shown in Table 3.
[0098] Note: M stands for CA heterozygote. A single A or C or G or T represents homozygosity.
[0099] From the identifica...
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Abstract
The invention relates to a SNP molecular marker for identifying Scutellaria baicalensis produced in Inner Mongolia and its method and application. The molecular marker includes 19 SNP molecular markers, and the nucleotide sequences of the 19 SNP molecular markers are as SEQ ID NO.1~SEQ Shown in ID NO.19. The SNP molecular marker for identifying Scutellaria baicalensis produced in Inner Mongolia and its method and application of the present invention use genomics technology to obtain SNP markers at the genome level of Scutellaria baicalensis, and can quickly and accurately determine whether the detected scutellaria baicalensis is produced in Inner Mongolia.
Description
technical field [0001] The invention belongs to the field of molecular biology molecular markers, and particularly relates to a SNP molecular marker for identifying Scutellaria baicalensis produced in Inner Mongolia and its method and application. Background technique [0002] Scutellaria baicalensis is a widely used traditional Chinese medicinal material. Its root is used as medicine. It is bitter in taste and cold in nature. Indications for febrile disease, upper respiratory tract infection, lung-heat cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle, swollen boils and other diseases. The clinical antibacterial activity of Scutellaria baicalensis is better than that of Coptis chinensis, and it does not produce drug resistance. According to relevant statistics, 70% of Chinese patent medicines currently on the market contain Scutellaria baicalensis, such as commonly used Chinese patent medicines Qingkailing...
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