Nucleic acid extraction and amplification test tube and working method thereof

A technology for extraction tubes and test tubes, applied in the field of nucleic acid extraction and amplification test tubes, can solve the problems of cumbersome, cumbersome operations, and inability to meet the needs of SARS-CoV2 detection

Active Publication Date: 2021-02-09
SUZHOU MOLARRAY CO LTD
View PDF13 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Automatic control, only need to add samples manually in the whole operation process; however, there are as many as 17 kinds of reagents, and the operation requires suction and rotation, which is cumbersome; moreover, it requires magnetic separation
[0013] It can be seen that in the prior art, most of the research still stays on how to extract nucleic acid; while a small amount of research on the integrated function of nucleic acid extraction and amplification has problems of cumbersome operation and complicated equipment design, which cannot meet the requirements of SARS- The need for CoV2 testing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid extraction and amplification test tube and working method thereof
  • Nucleic acid extraction and amplification test tube and working method thereof
  • Nucleic acid extraction and amplification test tube and working method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment one, refer to figure 1 As shown, a nucleic acid extraction and amplification test tube includes: PCR reaction tube 1, extraction tube assembly lower tube 2, extraction tube assembly middle tube 3, extraction tube assembly upper tube assembly 4, top cover 5, piston rod 6, the second A spring 7, a piston ring 8, a first spool 9, a second spring 10, a second spool 11, and a third spring 12;

[0102] Wherein, the top of the PCR reaction tube 1 is provided with an opening, and the top opening of the PCR reaction tube 1 is connected to the bottom of the lower tube 2 of the extraction tube assembly;

[0103] The upper pipe assembly 4 of the extraction pipe assembly, the middle pipe 3 of the extraction pipe assembly, and the lower pipe 2 of the extraction pipe assembly are sequentially connected into one body from top to bottom; a top cover 5 is connected to the top of the extraction pipe of the upper pipe assembly 4 of the extraction pipe assembly;

[0104] At the...

Embodiment 2

[0155] Embodiment 2, the purpose of embodiment 2 is to realize fully automatic process from sample collection to qPCR result output;

[0156] One-step POCT instant nucleic acid detection device, including: PCR instrument 101, extraction tube fixing plate 102, piston rod fixing plate 103, two motors 104, coupling 105, bearing housing 106, two circular guide rails 107, ball screw 108, screw nut 109, linear bearing 110, PCR reaction tube fixing seat;

[0157] A plurality of PCR reaction tube fixing seats are arranged on the upper surface of the PCR instrument 101, and the PCR reaction tube fixing seats are adapted to the shape of the PCR reaction tube;

[0158] The output shafts of the two motors are connected to the coupling 105, and the coupling 105 is connected to the ball screw 108;

[0159] Two circular guide rails 107 are arranged in parallel and the axis direction is vertical;

[0160] The extraction tube fixing plate 102 and the piston rod fixing plate 103 are arranged in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a nucleic acid extraction and amplification test tube and a working method thereof, and belong to the field of molecular diagnosis and detection instruments. According to key points of the design, the nucleic acid extraction and amplification test tube comprises a PCR reaction tube, an extraction tube assembly lower tube, an extraction tube assembly middle tube, an extraction tube assembly upper tube assembly, a top cover, a piston rod, a piston ring, a first valve core, a second spring, a second valve core and a third spring; the extraction tube assembly upper tube assembly, the extraction tube assembly middle tube, the extraction tube assembly lower tube and the PCR reaction tube are sequentially connected from top to bottom; the extraction tube assembly upper tube assembly comprises two vertical parallel tube bodies, namely an extraction tube and a piston tube; the top cover is arranged at the top end of the extraction tube; and the piston rod is arranged inthe piston tube. According to the nucleic acid extraction and amplification test tube and the working method thereof, the detection efficiency is greatly improved.

Description

technical field [0001] The present invention relates to the field of a medical inspection instrument and a molecular diagnosis instrument, and more specifically relates to a nucleic acid extraction and amplification test tube and its working method. Background technique [0002] Current methods for detecting and diagnosing microbial infections involve the use of quantitative polymerase chain reaction (qPCR) to amplify nucleic acid (RNA / DNA) in patient samples to confirm the presence of pathogens. Additionally, these tests must be done by a testing laboratory where two steps must be performed for diagnosis: nucleic acid extraction followed by qPCR. These two procedures may also be performed by different devices, adding to the complexity and length of time required between sample collection and diagnosis. Finally, these laboratory tests are often done manually, involving physical labor and potentially additional hazards when handling pathogen-positive samples. [0003] Curre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/04C12M1/00
CPCB01L7/52
Inventor 王威梅宗羲聂晶
Owner SUZHOU MOLARRAY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap