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Kit and method for determining renin activity of human plasma sample

A renin activity and kit technology, applied in the medical field, can solve the problems of narrow linear range, high cost of testing instrument consumables, poor method specificity, etc., to ensure accuracy and reproducibility, saving manpower and working time, and convenient. Finding the effect of the problem

Pending Publication Date: 2021-02-09
杭州凯莱谱精准医疗检测技术有限公司
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Problems solved by technology

Immunoassays for measuring renin activity have inherent defects in immunoassays, such as poor specificity, cross-coupling reactions, low sensitivity, narrow linear range, and complicated pretreatment processes, which cannot meet the requirements of the actual clinical conditions for patients with primary aldophyllism. Need for detection of low-level PRA
However, the existing high-performance liquid chromatography-mass spectrometry detection method requires more complex pre-treatment solid-phase extraction (SPE) or an additional or modified two-dimensional liquid chromatography system. The method is more complicated, the cost of detection instruments and consumables is high, and the technology is difficult. High, not suitable for the needs of clinical large-scale sample processing, and it is difficult to promote
In addition, the results of renin activity detection are easily affected by experimental conditions, such as buffer salt concentration, pH, plasma matrix, incubation temperature, etc., and often encounter detection deviations between chambers and chambers, how to control the accuracy and weight of results and data The reproducibility is also an important and difficult problem in the existing renin activity detection

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  • Kit and method for determining renin activity of human plasma sample
  • Kit and method for determining renin activity of human plasma sample
  • Kit and method for determining renin activity of human plasma sample

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Embodiment 1

[0033] Example 1: Analytical method for detecting renin activity in human plasma samples using liquid chromatography tandem mass spectrometry

[0034] 1. Prepare the solution

[0035] Preparation of stock solutions

[0036] Angiotensin I primary stock solution: Accurately weigh 1 mg of angiotensin I standard substance, dissolve it in 10 mL of 50% methanol aqueous solution, and prepare it as a primary stock solution of 100 μg / mL.

[0037] Preparation of the secondary stock solution of angiotensin I: draw 100 μL of the primary stock solution of angiotensin I, and dilute it with 900 μL of 50% aqueous methanol to obtain a secondary stock solution of 10 μg / mL.

[0038] Note: The above-prepared stock solutions are stored in a -20°C refrigerator.

[0039] Preparation of standard curve working solution:

[0040](1) Aspirate 100 μL of the secondary stock solution of angiotensin I, and dilute it with 900 μL of 50% aqueous methanol to obtain a tertiary stock solution of 1 μg / mL.

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Abstract

The invention relates to a kit and a method for determining renin activity of a human plasma sample. The kit comprises (1) a standard curve working solution preparation kit, (2) an internal standard working solution preparation kit, (3) a sample pretreatment buffer solution preparation kit, (4) a quality control sample configuration kit, (5) a reaction stop solution kit, and (6) a liquid chromatography gradient eluent. The kit and the method have the advantages of high efficiency, low cost, simplicity and convenience in operation, high accuracy, high sensitivity and the like.

Description

technical field [0001] The invention relates to the field of medical technology, and mainly relates to a kit and a method for measuring renin activity of human plasma samples. Background technique [0002] Plasma renin activity (Plasma Renin Activity, PRA) is an index to measure the production efficiency of angiotensin I in peripheral blood under the catalysis of renin. Its activity is significantly increased in hypertensive patients caused by renal artery stenosis, and decreased in patients with secondary aldosteronism. In patients with adrenal insufficiency, renin activity is also an important measure of the effectiveness of hormone replacement therapy, with normal values ​​in patients with adequate hormone replacement therapy and elevated values ​​in patients with insufficient response to hormone replacement therapy. Renin activity is also the most important index for primary aldosteronism (Primary Aldosteronism, referred to as PA) screening. Currently, immunoassay tech...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/72
CPCG01N30/02G01N30/34G01N30/72
Inventor 刘鹏云马金飞曲亮沈凌晓刘华芬
Owner 杭州凯莱谱精准医疗检测技术有限公司
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