Separation and purification method of high-purity ecdysone

Abstract

The invention relates to the technical field of separation and purification of plant effective components, and discloses a separation and purification method of high-purity ecdysone. The method comprises the following steps: material selection: selecting 1-2-year-old cyanotis arachnoidea which has no rotting, mildewing and visible impurities, and cleaning, airing, peeling and drying the roots of the cyanotis arachnoidea to obtain the dry roots of the cyanotis arachnoidea to serve as raw materials; mashing: putting the raw materials into a masher for mashing, and then sieving with a 60-mesh sieve to obtain cyanotis arachnoidea powder; extracting and separating: separating the cyanotis arachnoidea powder in a high performance liquid chromatograph, adding an inducer, and carrying out extracting to obtain an ecdysone sample; and purifying: adsorbing the ecdysone sample by using a clarifying agent to obtain high-purity ecdysone. By adopting the CH3COOC2H5 inducer and the magnetic nanoparticle clarifying agent, the recovery rate and purity of ecdysone can be greatly improved, the cost is low, and large-scale production of ecdysone is facilitated.

Description

technical field [0001] The invention relates to the technical field of separation and purification of plant active ingredients, in particular to a method for separation and purification of high-purity ecdysone. Background technique [0002] Chinese invention patent CN103788162A discloses a method for extracting ecdysteroids from spinach. Take spinach leaves, grind them into powder in liquid nitrogen, add a volume ratio of 9:1 MeOH-CHCl3 mixed solution or 100% MeOH solution to 50ml, and heat Shake at C for 2 hours, filter, extract twice, collect the MeOH layer, dry it under reduced pressure, redissolve the sample with isopropanol, and perform HPLC analysis after filtration to determine its content. However, this method is only limited to small-scale production in the laboratory, and has limitations on the freshness of spinach raw materials. It cannot meet the production requirements in the season when spinach is not on the market in a large area; , water keel, etc. to extrac...

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Problems solved by technology

However, this method is only limited to small-scale production in the laboratory, and has limitations on the freshness of spinach raw materials. It cannot meet the production requirements in the season when spinach is not on the market in a large area; , water keel, etc. to extract ecdysteroids, seldom extract ecdysteroids from spinach; ecdysterone is an active substance extracted from the root of Commelinaceae plant Pearl Dew, and is an endogenous sterol compound. According to According to different purity, it can be divided into white, off-white, light yellow or light brown crystalline powder, which is widely used in breeding, health care, cosmetics, and pharmaceutical industries; but among the ecdysone products on the market today, the purity of ecdysone is not enough, which is easy to cause negative effects , so it is particularly important to invent a method for the separation and purification of high-purity ecdysone

[0003] Nowadays, most of the separation and purification methods of ecdysone use macroporous resin columns to separate and purify ecdysone, which increases the cost and complicated process, and the thickness of the obtained ecdysone is not high, so it is impossible to carry out one-time large-scale separation and purification. For this reason, we propose a kind of separation and purification method of high-purity ecdysone

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