Paenibacillus with yield increasing effect and preparation method and application thereof

A technology of Paenibacillus and Bacillus, applied in the field of Paenibacillus and its preparation, can solve the problems of easily damaged soil, chemical agent residues on crops, and affecting the effect of increasing production, so as to reduce pollution, improve crop quality, and increase production. Effect

Pending Publication Date: 2021-02-26
广西绿友农生物科技股份有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Aiming at the deficiencies of the prior art, the present invention provides a Paenibacillus with yield-increasing effect and its preparation method and application, which overcomes the deficiencies of the prior art, has a reasonable design and a compact structure, and solves the problems of fertilization and use of pesticides. Insects and fungicides are used to increase the yield of crops, which will easily damage the soil and at the same time chemical agents will remain on the crops, which will affect the effect of increasing yield. The present invention adopts a kind of Paenibacillus and prepares Paenibacillus into a bacterial agent, which can treat many Planting crops can improve the effect of increasing production, the preparation of bacterial agents is simple, the fermentation time is short, the application is convenient, and the efficiency of increasing production is high. At the same time, it has the advantages of increasing crop yield and improving crop quality, improving soil, protecting the ecological environment, and reducing pollution.

Method used

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  • Paenibacillus with yield increasing effect and preparation method and application thereof
  • Paenibacillus with yield increasing effect and preparation method and application thereof
  • Paenibacillus with yield increasing effect and preparation method and application thereof

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Effect test

preparation example Construction

[0032] A method for preparing a crop yield-increasing bacterial agent, comprising the steps of:

[0033] Step 1: Streak the strain on LB solid medium for activation culture at 20-37°C.

[0034] Step 2: Pick a single colony and inoculate it into LB liquid medium, and culture it on a shaker at 20-37°C and 100-300r / min for 1-3 days.

[0035] Step 3: The fermented strain liquid fermentation broth can be used directly after dilution.

Embodiment 1

[0037] Experimental materials: ginger, bacterial agents of the above strains, LB medium

[0038] Experimental settings: water control group, 1ml LB blank control group, 1ml of the above bacterial agent treatment group. Each group was repeated 5 times, and the inoculum was watered once every 2 weeks.

[0039] Experimental process: In this experimental case, after pouring the fungus 10 times, wait for the ginger to mature, which lasted 4 months, pull it out and clean it, measure the yield of each plant, and calculate the average plant yield of each group.

[0040] In this case, there was no difference in the growth and development of the three groups, but when the average yield per plant was finally measured, the group that applied 1ml of the above bacterial agent had a 45.29% increase in production compared to the 1ml LB blank control group; The yield of the control group increased by 37.97%.

Embodiment 2

[0042] Experimental materials: eggplant, bacterial agents of the above strains, LB medium

[0043] Experimental settings: water control group, 10ml LB blank control group, 10ml of the above bacterial agent treatment group. Each group was repeated 5 times, and the inoculum was watered once every 2 weeks.

[0044] Experimental process: In this experimental case, the fungus was poured 10 times, which lasted 3 months, and the fruit was picked 4 times, and all the fruits were picked each time, the yield of each plant was measured, and the average plant yield of each group was calculated.

[0045] In this case, there was no difference in the growth and development of the three groups, but when the average yield per plant was finally measured, the group that applied 1ml of the above bacterial agent had a 45.29% increase in production compared to the 1ml LB blank control group; The yield of the control group increased by 37.97%.

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Abstract

The invention relates to the technical field of paenibacillus and application thereof, and provides paenibacillus with a yield increasing effect and a preparation method and application thereof. The paenibacillus is prepared into a microbial agent, and the microbial agent can effectively promote plant yield increasing. The invention further provides a method for preparing the microbial agent. Thedefects in the prior art are overcome, and the problems that in existing fertilization and use of insecticides and bactericides for crop yield increasing, soil is easily damaged, meanwhile, chemical agents can also be left on crops, and the yield increasing effect is affected are solved. The paenibacillus is adopted and prepared into the microbial agent, the yield increasing effect on various crops can be improved, the microbial agent is easy to prepare, short in fermentation time and convenient to apply, meanwhile, the yield increasing efficiency is high, and the advantages of increasing thecrop yield, improving the crop quality, improving the soil, protecting the ecological environment, reducing pollution and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of Paenibacillus and its application, in particular to a Paenibacillus with production-increasing effect and its preparation method and application. Background technique [0002] Crops, including the yield of crops, have always been a worldwide problem. At present, in the process of agricultural production, fertilization and the use of insecticides and fungicides are generally used to increase crop production. [0003] Long-term use of chemical fertilizers, due to the lack of organic matter and humus in the soil, will destroy the soil aggregate structure, cause soil compaction, and reduce the yield of agricultural plants. In addition, extensive use of chemical fertilizers will greatly reduce the quality of vegetables and fruits. Since crops not only need nitrogen, phosphorus and potassium, but also many trace elements such as calcium, iron, zinc and selenium, and chemical fertilizers generally have relativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01B79/00A01G7/06C12R1/01
CPCA01B79/00A01G7/06C12N1/20C12N1/205C12R2001/01
Inventor 卢杰黄文善姜明国朱英芝申乃坤王一兵黄群刘艳
Owner 广西绿友农生物科技股份有限公司
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