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Kit for detecting human lansoprazole sensitive genes

A human lansoprazole and kit technology, applied in the field of biomedicine, can solve the problems of high detection cost and low detection throughput, and achieve the effect of high throughput, low cost and rapid detection

Inactive Publication Date: 2021-02-26
为康(苏州)基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection technology of lansoprazole metabolism gene in the market is mostly carried out by fluorescent PCR and other technologies; the detection throughput is small, and the detection cost is high

Method used

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  • Kit for detecting human lansoprazole sensitive genes
  • Kit for detecting human lansoprazole sensitive genes
  • Kit for detecting human lansoprazole sensitive genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] In this example, human lansoprazole drug-sensitive gene mutation sites CYP2C19-1, CYP2C19-2, and CYP2C19-3 are used as detection objects, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid Simultaneous detection of CYP2C19-1, CYP2C19-2, and CYP2C19-3 three gene loci.

[0051] The gene template of the experiment is: 30 wild-type blood samples and 5 mutant blood samples, which are tested at the same time, and finally the correct rate of negative and positive is used as the standard.

[0052] The first step, multiplex PCR reaction:

[0053] Primer set a mix (including primers CYP2C19F1, CYP2C19R1, CYP2C19F2, CYP2C19R2, CYP2C19F3, CYP2C19R3) 1ul

[0054] PCR master Mix 1.2ul

[0055] H2O 0.8ul

[0056] DNA template 2ul

[0057] The reaction conditions of the multiplex PCR reaction are: 95° C. for 2 minutes, 95° C. for 30 seconds, 56° C. for 20 seconds, 72° C. for 60 seconds, 45 cycles, 72° C. for 5 minutes...

Embodiment 2

[0066]In this example, human lansoprazole drug-sensitive gene mutation sites CYP2C19-1, CYP2C19-2, and CYP2C19-3 are used as detection objects, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid Simultaneous detection of CYP2C19-1, CYP2C19-2, and CYP2C19-3 three gene loci.

[0067] The gene template of the experiment is: 1 wild-type blood sample and 1 mutant blood sample, and 20 blood samples and 10 saliva samples are tested simultaneously.

[0068] Utilize above-mentioned multiplex PCR to detect the method for human lansoprazole medication sensitive gene mutation locus to comprise the following steps:

[0069] (1) Sample processing and template extraction quality control:

[0070] The scope of application of samples includes whole blood, saliva, oral test strips and other specimens; for the extraction process, please refer to the instructions of the relevant finished commercial kits. This experiment takes the e...

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Abstract

The invention discloses a kit for detecting human lansoprazole sensitive genes. The kit comprises a CYP2C19-1 primer group, a CYP2C19-2 primer group and a CYP2C19-3 primer group. The drug sensitive genes CYP2C19 for human lansoprazole are used as a detection object. Through a combination of the specific primers and combination of a nucleic acid mass spectrometry detection technology, a rapid, simple and accurate detection of variation conditions of the related genes is realized..

Description

technical field [0001] The invention relates to a kit for detecting human lansoprazole sensitive genes, belonging to the technical field of biomedicine. Background technique [0002] Pharmacogenomic studies have shown that the metabolic level of lansoprazole in the body is closely related to the phenotypes of genes such as CYP2C19 of the cytochrome P450 family; FDA also proposed this point in the description of the drug; therefore, pre-medication testing is relevant The mutation of the gene can effectively assist the clinical medication of lansoprazole; it has important guiding significance for patients. [0003] At present, most of the detection technologies for lansoprazole metabolism genes in the market are carried out by fluorescent PCR and other technologies; the detection throughput is small and the detection cost is high. [0004] The present invention aims to check the variation of human diabetes medication sensitive genes through the nucleic acid mass spectrometry ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 田军龙张为
Owner 为康(苏州)基因科技有限公司
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