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Application of Phenidone in Improving Salt Tolerance of Plants

A technology of phenidone and salt tolerance, which is applied in the field of application of phenidone in improving the salt tolerance of plants, can solve the problems of unseen plant stress resistance, so as to improve planting income and salt stress resistance , Concentration setting scientific and reasonable effect

Active Publication Date: 2022-04-01
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of botany, there are currently no patents or reports on phenidone directly affecting plant stress resistance

Method used

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  • Application of Phenidone in Improving Salt Tolerance of Plants
  • Application of Phenidone in Improving Salt Tolerance of Plants
  • Application of Phenidone in Improving Salt Tolerance of Plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Phenidone can promote millet germination under salt stress conditions

[0033] 1. Prepare 16.219mg of ibuprofen into 100mM mother solution with 1mL of absolute ethanol, and then dilute with water into 50μM and 100μM fenidone solutions respectively, and use it as the subsequent treatment solution.

[0034] 2. Select plump and healthy millet seeds, first disinfect the surface of the seeds with 70% ethanol for 20 seconds, and then wash them with sterile water for 5-6 times. Millet seeds were divided into blank group (CK), control group and experimental group. The seeds of CK group were soaked in water for 12 hours for pretreatment, and the seeds of control group and experimental group were soaked in 150mM NaCl solution for 12 hours for pretreatment. Three groups of parallel experiments were set up in each group, and 80 millet seeds were selected in each parallel. Spread the soaked seeds on a 9 cm glass petri dish with sterilized filter paper placed in advanc...

Embodiment 2

[0036] Embodiment 2: Phenidone can improve the salt tolerance of millet hydroponic seedlings

[0037] 1. Prepare 16.219 mg of ibuprofen with 1 mL of absolute ethanol to prepare a 100 mM mother solution, and then dilute with water to obtain phenidone treatment solutions with concentrations of 10 μM, 50 μM, and 100 μM.

[0038] 2. Select plump and healthy millet seeds, first disinfect the surface of the seeds with 70% ethanol for 20 seconds, and then wash them with sterile water for 5-6 times. Three days after millet germination, select seedlings with consistent growth and spread them on 9 cm glass petri dishes with sterilized filter paper placed in advance, 10 per dish, and 5 mL of treatment solution. Divided into blank group (CK), control group and experimental group, the seedlings of CK group were treated with clear water, the seedlings of control group were treated with 150mM NaCl solution, and the experimental group were treated with 200mM NaCl containing 10μM, 50μM and 100...

Embodiment 3

[0040] Example 3: Exogenous spraying of phenidone can improve the salt tolerance of millet seedlings

[0041] 1. Prepare 16.219 mg of ibuprofen into 100 mM mother solution with 1 mL of absolute ethanol, and then dilute with water to obtain phenidone treatment solutions with concentrations of 50 μM, 100 μM, and 500 μM.

[0042] 2. Choose plump and healthy millet seeds. 15 millet seeds were planted in each black seed tray, and after the millet germinated for 3 days, 9 seedlings with consistent growth were selected, and the remaining seedlings were removed with tweezers simultaneously. The 9 seedlings were treated after continuing to grow for 3 days. The seedlings of the CK group were irrigated with clear water, and the seedlings of the control group and the experimental group were irrigated with an equal amount of 200mM NaCl solution. Then, the seedlings of the CK group and the control group were sprayed with clear water, and the seedlings of the experimental group were sprayed...

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Abstract

The invention discloses the application of phenidone in improving the salt tolerance of plants. The phenidone has the ability to improve the plant's ability to resist salt stress. The present invention prepares phenidone into a safe concentration of phenidone solution, and applies it to plant seeds and seedlings subjected to salt stress by soaking, watering or spraying. ; Phenidone solution can significantly improve the germination rate of plant seeds under salt stress, significantly improve the survival rate, plant height, root length and fresh weight of plant seedlings under salt stress, and improve the ability of plants to resist salt stress; exogenous spraying And watering fenidone can also significantly improve the salt tolerance of plants, increase plant height, root length and fresh weight, and fenidone is easy to use, effective, safe and reliable, very conducive to plant growth and increase planting income.

Description

technical field [0001] The invention belongs to the field of plant planting, and in particular relates to the application of phenidone in improving the salt tolerance of plants. [0002] technical background [0003] Soil salinization seriously affects the growth and quality of crops, making it one of the main environmental factors limiting crop yields today. Various life activities of plants are affected to varying degrees under salt stress, mainly manifested as inhibiting plant growth and development, destroying plant cell structure, and affecting the synthesis of biomolecules required to maintain normal plant physiological metabolism. When there is too much salt in the soil, it usually causes physiological drought, ion poisoning, and disruption of normal metabolism to plants. High salt affects seed germination, seedling growth and vigor, and plant flowering and fruiting, ultimately leading to a decline in crop economic yield. Efficient use of salinized soil and improveme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06A01G13/00A01G22/20A01G22/45A01N43/56A01P21/00
CPCA01G7/06A01G13/00A01G22/20A01G22/45A01N43/56
Inventor 吴长艾肖圣慧万夷曼王琰白依超郑成超
Owner SHANDONG AGRICULTURAL UNIVERSITY
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