Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of amphiphilic polymer for surface biofunctionalization and its preparation method and application

A polymer, amphiphilic technology, applied in the field of polymers, can solve the problems of harsh conditions for block copolymerization, restricting block polymers, and difficult to change flexibly, so as to expand different biomedical applications, achieve diversity, and avoid cumbersome and inefficient effects

Active Publication Date: 2021-07-23
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
View PDF18 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biofunctionalization of hydrophobic substrates using amphiphilic block polymers has its limitations: (1) The ratio of each block needs to be set ab initio when synthesizing different block copolymers, and any adjustment to the copolymerization ratio requires resynthesis ; (2) The type of block part is fixed, and it is difficult to flexibly change according to the properties of the substrate; (3) The conditions of block copolymerization are relatively harsh, and high-purity organic solvents and high-temperature reaction conditions are usually used
These deficiencies limit the application of block polymers for diverse biofunctionalization of surface hydrophobic substrates

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of amphiphilic polymer for surface biofunctionalization and its preparation method and application
  • A kind of amphiphilic polymer for surface biofunctionalization and its preparation method and application
  • A kind of amphiphilic polymer for surface biofunctionalization and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0117] The preparation method of the present invention will be further described in detail below in conjunction with specific examples. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0118] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the reagents and materials used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0119] The HEPES buffer used in the following examples is a buffer solution with a concentration of 100 mM and a pH of 7.0.

[0120]Optional means that the feature exists or does not exist, and also means that the feature must exist, but the spe...

Embodiment 1

[0121] Example 1 Synthesis of amphiphilic polymer OA25-PLL-PEG2k-NTA30

[0122] Weighed 8.5 mg of poly-L-lysine (PLL, average molecular weight 22,500 Da, purchased from Shanghai Yuanye Company) in a 2 ml centrifuge tube, and dissolved it in 200 μL of HEPES buffer to obtain a solution (1).

[0123] Weigh 24 mg of polyethylene glycol 2000 (NHS-PEG2k-NTA) modified with succinimidyl ester (NHS) and nitrilotriacetic acid (NTA) at both ends respectively, and the average molecular weight of polyethylene glycol is 2000Da, purchased from Nanocs Corporation of the United States. ), which was dissolved in 100 μL N,N-dimethylformamide (analytical grade, Beijing Chemical Plant) to obtain solution (2).

[0124] Measure 3.2 μL of oleic acid (molecular weight 282.5, purchased from Beijing Chemical Plant, purified by distillation) in a glass vial, and place it in 100 μL N,N-dimethylformamide to obtain solution (3).

[0125] Mix the above solution (1), solution (2) and solution (3) in a centri...

Embodiment 2

[0129] Example 2 Synthesis of amphiphilic polymer OA25-PLL-PEG5k-NTA30

[0130] Similar to Example 1, the difference is that 60 mg of polyethylene glycol 5000 (NHS-PEG5k-NTA, polyethylene glycol with an average molecular weight of 5000 Da, purchased from the U.S. Nanocs Company), which was dissolved in 100 μL N,N-dimethylformamide to obtain solution (2). 37 mg of white lyophilized powder were obtained.

[0131] The white powder is a functionalized amphiphilic polymer grafted with 25% oleic acid and 30% polyethylene glycol 5k-nitrilotriacetic acid, and the polymer is named OA25-PLL-PEG5k-NTA30.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The present invention provides an amphiphilic polymer for surface biological functionalization and its preparation method and application. The amphiphilic polymer overcomes the block amphiphilic polymer by introducing hydrophilic components and hydrophobic components. Due to limitations, the amphiphilic polymer has the following advantages: the use of amphiphilic functional polymers only needs to cover the hydrophobic material in one step to obtain a stable biofunctionalized surface and realize the efficient preparation of biofunctionalized materials. Amphiphilic functional polymers can flexibly adjust the number and types of hydrophilic groups and hydrophobic groups through side chain modification to meet the needs of biofunctionalization of substrates with different properties. It is also possible to introduce different biological functional groups through the side chain modification of amphiphilic functional polymers, realize the diversity of biological functionalization of substrates, and expand different biomedical applications.

Description

technical field [0001] The invention belongs to the field of polymers, and in particular relates to an amphiphilic polymer used for surface biofunctionalization, a preparation method and application thereof. Background technique [0002] Biomedical materials have been widely used in biological analysis, medical diagnosis, repair and replacement of organ tissues. Membrane-based biomedical materials are used in biological detection chips (including protein chips, DNA chips) and biological microfluidic devices. Various 2D and 3D biomedical materials have been used in tissue engineering for regenerative medicine, forming a huge medical technology market. The application of biomedical materials in various aspects puts forward diversified requirements for their biocompatibility (biocompatibility, that is, maintaining the activity of biomolecules and low cytotoxicity) and biofunctionality. Biofunctionalization is to immobilize functional units with physiological activity on bioco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00G01N21/552
CPCC08G81/00G01N21/553
Inventor 喻盈捷杨雷王宇舒游长江李泽浩聂国辉
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN