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Ramaria sp.stock culture medium, application thereof, as well as ramaria sp.stock and culture method thereof

A kind of original seed culture medium and the technology of mycelium, which is applied in the original culture medium of mycelia, the cultivation of original species of mycelium, and the field of original species of mycelium, which can solve the problems of sparse mycelia, slow growth of mycelium, and Problems such as the inability to carry out artificial domestication and cultivation experiments to achieve the effect of strong mycelia and fast growth

Active Publication Date: 2021-03-05
北京市房山区种植业技术推广站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the studies on Cladophora are limited to resource investigation or research on macromolecular biological activity. During the test, it was found that the mycelium of the fungus grows very slowly on the conventional original seed medium, and the mycelium is sparse, and even does not eat food and does not germinate, so it is impossible to carry out subsequent artificial domestication cultivation experiments

Method used

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  • Ramaria sp.stock culture medium, application thereof, as well as ramaria sp.stock and culture method thereof
  • Ramaria sp.stock culture medium, application thereof, as well as ramaria sp.stock and culture method thereof
  • Ramaria sp.stock culture medium, application thereof, as well as ramaria sp.stock and culture method thereof

Examples

Experimental program
Comparison scheme
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Embodiment approach

[0032] According to a preferred embodiment of the present invention, the stock culture medium contains fermented birch chips, wheat bran, calcium sulfate (gypsum), potassium dihydrogen phosphate and magnesium sulfate.

[0033] According to a preferred embodiment of the present invention, the stock medium is composed of fermented birch chips, wheat bran, calcium sulfate (gypsum), potassium dihydrogen phosphate, magnesium sulfate and water.

[0034] According to the present invention, the preparation method of the stock culture medium is not particularly limited, as long as each component can be mixed uniformly, and the order of adding each component can also be adjusted arbitrarily. According to a preferred embodiment of the present invention, after mixing the components uniformly, they are piled up for 0.5-1.5 hours.

[0035] In the second aspect, the present invention provides the application of the stock culture medium as described above in the stock culture of Cladophora fu...

preparation example 1

[0058] This preparation example is used to illustrate the acquisition of the pure culture of Cladocoronas CGMCC NO: 17783 mycelium

[0059] The collected fruiting bodies with the preservation number CGMCC NO: 17783 (such as figure 1 shown), put it on a clean workbench, blow aseptic air for 30cm, disinfect the operator’s hands with 75% alcohol, and carry out surface disinfection and flame sterilization on the operating tools, etc., use 75% Wipe the roots and main branches with alcohol cotton balls, carry out surface disinfection, and break off the first-level branches by hand (such as figure 1 The part marked by the middle arrow), and break off the branch from the base to the top by hand, so that the branch is divided into two halves. Be careful not to touch the exposed bacteria with your hands. Clamp the 0.3cm long and 0.2-0.3cm wide bacterial flesh, put it into the sterilized test tube containing PDA slant medium, place the bacterial flesh tissue in the middle of the slant, ...

preparation example 2

[0061] This preparation example is used to illustrate the acquisition of the mycelia pure culture of reference

[0062] The fruiting body of another strain of Cladophora (reference Cladophora) collected at the same time was placed on the ultra-clean workbench, and the aseptic wind was blown for 30 cm, and the operator's hands were disinfected with 75% alcohol, and the operating tools, etc., were surface disinfected and cleaned. For flame sterilization, wipe the root and main branch of the fruiting bodies of the reference Cladophorus with 75% alcohol cotton balls for surface disinfection, break off the first-level branches by hand, and break off the broken branches from the base to the top by hand , split the branch into two halves, and be careful not to touch the bare flesh with your hands. Use sterilized tweezers to pick up the 0.3cm long and 0.2-0.3cm wide bacterial flesh from the base of the main branch, and put it into the sterilized bacteria containing In the PDA slant me...

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Abstract

The invention relates to the technical field of edible fungi, and discloses a ramaria sp.stock culture medium and an application thereof, as well as a ramaria sp.stock and a culture method thereof. The stock culture medium contains sawdust, wheat bran, calcium salt, phosphate and magnesium salt. Through ramaria sp.stock culture in the stock culture medium provided by the invention, compared with the ramaria sp.cultured in a conventional stock culture medium, the ramaria sp.cultured in the stock culture medium provided by the invention has the advantages that the growth speed of the ramaria sp.is higher, hyphae are healthier and stronger, aerial hyphae are more uniform, the color is better, and even sclerotia occurs. Compared with other ramaria sp., the stock culture medium disclosed by the invention is more suitable for ramaria sp. with the preservation number of CGMCC NO: 17783.

Description

technical field [0001] The present invention relates to the technical field of edible fungi, in particular to a culture medium for the original species of Cladophorus fungi, the application of the culture medium for the original species of Cladocarpus fungi, a method for cultivating the original species of Cladocarpus fungus, and The original species of Cladophyllum cultivated by the method. Background technique [0002] Wild Ramaria sp. (Ramaria sp.), also known as broom fungus, brush fungus, belongs to the order Aphyllobacteriaceae, Ramariaceae, and the genus Ramaria sp., mostly grows in broad-leaved forests or coniferous forests in mountainous areas in summer and autumn. Each genera of this family contains many edible fungi with unique flavors, which are valuable components of wild edible fungus resources in my country. According to reports, active substances such as polysaccharides from Cladophyllum polysaccharides have functions such as tumor inhibition, anti-oxidation, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14Y02W30/40
Inventor 郭永杰张玉铎杨玉娟张东雷徐凯刘杰
Owner 北京市房山区种植业技术推广站