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Method for extracting pharmaceutical leucine by using solvent method

A technology of leucine and pharmaceutical grade, applied in the field of extracting pharmaceutical grade leucine by solvent method, can solve the problems of low purity of L-leucine, and achieve the effect of solving the problem of mass production

Pending Publication Date: 2021-03-09
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of low purity of L-leucine in industrial extraction, the present invention provides a method for extracting pharmaceutical grade leucine by solvent method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for extracting pharmaceutical-grade leucine using a solvent method. The main experimental equipment and reagents for the experimental extraction include: a rotary evaporation concentrator, a centrifuge, a plate frame, 10L of leucine fermentation broth, o-xylene-4-sulfonic acid Acid 300g, ammonia water 30ml, activated carbon 10g, deionized water, etc.

[0039] (1) Take 10L of leucine fermentation broth, centrifuge it at 5000rpm for 4min with a high-speed disc centrifuge, collect the filtrate and bacterial protein precipitation, and concentrate the filtrate at 60°C to produce 5L of water;

[0040] (2) Cool down the concentrated solution to 20-30°C, add 300g of o-xylene-4-sulfonic acid, stir for 30min, let it stand for 2h, and react to form leucine-xylene-4-sulfonate;

[0041] (3) Discard the supernatant, add 8 times the remaining volume of deionized water to wash twice, then add 8-10 times of deionized water, heat up to 70-80°C, add 30ml of ammonia water while st...

Embodiment 2

[0047] A method for extracting pharmaceutical-grade leucine using a solvent method. The main experimental equipment and reagents for the experimental extraction include: a rotary evaporation concentrator, a centrifuge, a plate frame, 100L of leucine fermentation broth, o-xylene-4-sulfonic acid Acid 3KG, ammonia water 300ml, activated carbon 100g, deionized water, etc.

[0048] (1) Take 100L of leucine fermentation broth, centrifuge at 5000rpm for 4min with a high-speed disc centrifuge, collect the filtrate and bacterial protein precipitation, and concentrate the filtrate at 60°C to produce 50L of water;

[0049] (2) Cool down the concentrated solution to 20-30°C, add 3KG of o-xylene-4-sulfonic acid, stir for 30 minutes, let it stand for 2 hours, and react to form leucine-xylene-4-sulfonate;

[0050] (3) Discard the supernatant, add 8-10 times the remaining volume of deionized water to wash twice, then add 10 times of deionized water, heat up to 70-80°C, add 300ml of ammonia wa...

Embodiment 3

[0056] A method for extracting pharmaceutical-grade leucine using a solvent method. The main experimental equipment and reagents for the experimental extraction include: a rotary evaporation concentrator, a centrifuge, a plate frame, 100L of leucine fermentation broth, o-xylene-4-sulfonic acid Acid 3.2KG, ammonia water 320ml, activated carbon 120g, deionized water, etc.

[0057] (1) Take 100L of leucine fermentation broth, and centrifuge it at 5000rpm for 4min with a high-speed disc centrifuge, collect the filtrate and bacterial protein precipitate, and concentrate the filtrate at 60°C to produce 40L of water;

[0058] (2) Cool the concentrated solution to 20-30°C, add 3KG of o-xylene-4-sulfonic acid, stir for 30 minutes, and let it stand for 1.5 hours to form leucine-xylene-4-sulfonate through chemical reaction;

[0059] (3) Discard the supernatant, add 8 times the remaining volume of deionized water to wash twice, then add 8-10 times of deionized water, heat up to 70-80°C, add...

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Abstract

The invention discloses a method for extracting pharmaceutical-grade leucine by using a solvent method. The method comprises the following specific processes: 1) preparing fermentation broth; 2) removing protein from the fermentation broth; 3) concentrating the fermentation broth; 4) carrying out a chemical reaction precipitation on o-xylene-4-sulfonic acid and the fermentation broth to generate leucine xylene-4-sulfonate; 5) dissolving the leucine xylene-4-sulfonate, adding ammonia water for hydrolysis, and removing o-xylene-4-sulfonic acid; 6) decoloring; and 7) drying, crushing, carrying out finished product detection, and packaging. According to the invention, in the production process, the method is simple and convenient to operate and high in separation purity, and effectively solvesthe problem of batch production of pharmaceutical leucine.

Description

technical field [0001] The invention belongs to the technical field of amino acid extraction, and relates to a method for extracting medical-grade leucine by using a solvent method. [0002] technical background [0003] L-leucine is also called leucine, its chemical name is α-aminoisocaproic acid, and its molecular formula is C 6 h 13 o 2 N, with a relative molecular weight of 131.18, is a non-polar amino acid with a slightly bitter taste, soluble in water, and an isoelectric point of 5.98. It is widely used in many industries such as medicine, food, seasoning, animal feed and cosmetics. my country's research on L-leucine started relatively late, some enterprises produced low levels of L-leucine by fermentation, and there were relatively few studies on downstream pretreatment and extraction processes, it was difficult to realize industrial production, and there were Problems such as high acid and alkali consumption, serious pollution, and low yield and purity of the curren...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C227/18C07C229/08C07C303/32C07C309/30
CPCC07C227/40C07C227/18C07C229/08C07C303/32C07C309/30
Inventor 边恩来冯世红张宗华郭晓东王奎智杨晓芳
Owner XINJIANG FUFENG BIOTECH
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