Dual-network adhesive for crosslinking of transglutaminase and preparation method of dual-network adhesive

A transglutaminase and adhesive technology, which is applied in the field of biomedical tissue engineering materials, can solve the problems of lack of adhesion ability of tissue surface acting groups, complicated and difficult to implement synthetic modification methods, complicated synthetic methods, etc. Low, simple synthetic method, good biocompatibility

Active Publication Date: 2021-03-16
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also disadvantages that the synthetic modification method is complicated and difficult to implement, and it lacks the limited ability to improve the binding ability of the tissue surface interaction group.
[0005] It can be seen that the existing enzymatic cross-linking adhesives still have the problems of complex synthesis methods, insufficient bulk strength, and limited adhesive capacity.

Method used

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  • Dual-network adhesive for crosslinking of transglutaminase and preparation method of dual-network adhesive
  • Dual-network adhesive for crosslinking of transglutaminase and preparation method of dual-network adhesive
  • Dual-network adhesive for crosslinking of transglutaminase and preparation method of dual-network adhesive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0044] The dextran powder that takes by weighing 2g number-average molecular weight 40000 is dissolved in 160ml deionized water, and is stirred at room temperature until fully dissolved; The NaIO that takes by weighing 1.9g Powder is dissolved in 40ml deionized water, is stirred until fully dissolved; NaIO solution is added to glucose In the polysaccharide solution, keep away from light, and keep stirring at a stirring rate of 200rpm for 24h; add 0.95g of diethylene glycol to the mixed solution of NaIO4 and dextran, and stir at room temperature for 1h; after the reaction is completed, dialyze in deionized water for 72h, -40 Dextran aldehyde with an oxidation degree of 50% was obtained after freeze-drying at ℃. Dissolve 11 mg of dextran aldehyde sample in 100 μl of phosphate buffer solution with pH=7.2-7.4, and stir until completely dissolved.

[0045] Weigh 1.5g of gelatin powder, dissolve it in 5ml of phosphate buffer solution with pH=7.2-7.4, let it stand in a water bath at ...

Embodiment example 2

[0048] The dextran powder that takes by weighing 2g number-average molecular weight 40000 is dissolved in 160ml deionized water, and is stirred at room temperature until fully dissolved; The NaIO that takes by weighing 1.9g Powder is dissolved in 40ml deionized water, is stirred until fully dissolved; NaIO solution is added to glucose In the polysaccharide solution, keep away from light, and keep stirring at a stirring rate of 200rpm for 24h; add 0.95g of diethylene glycol to the mixed solution of NaIO4 and dextran, and stir at room temperature for 1h; after the reaction is completed, dialyze in deionized water for 72h, -40 Dextran aldehyde with an oxidation degree of 50% was obtained after freeze-drying at ℃. Dissolve 11 mg of dextran aldehyde sample in 100 μl of phosphate buffer solution with pH=7.2-7.4, and stir until completely dissolved.

[0049] Weigh 1.5g of gelatin powder, dissolve it in 5ml of phosphate buffer solution with pH=7.2-7.4, let it stand in a water bath at ...

Embodiment example 3

[0052] The dextran powder that takes by weighing 2g number-average molecular weight 40000 is dissolved in 160ml deionized water, and is stirred at room temperature until fully dissolved; The NaIO that takes by weighing 1.9g Powder is dissolved in 40ml deionized water, is stirred until fully dissolved; NaIO solution is added to glucose In the polysaccharide solution, keep away from light, and keep stirring at a stirring rate of 200rpm for 24h; add 0.95g of diethylene glycol to the mixed solution of NaIO4 and dextran, and stir at room temperature for 1h; after the reaction is completed, dialyze in deionized water for 72h, -40 Dextran aldehyde with an oxidation degree of 50% was obtained after freeze-drying at ℃. Dissolve 11 mg of dextran aldehyde sample in 100 μl of phosphate buffer solution with pH=7.2-7.4, and stir until completely dissolved.

[0053] Weigh 1.5g of gelatin powder, dissolve it in 5ml of phosphate buffer solution with pH=7.2-7.4, let it stand in a water bath at ...

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Abstract

The invention discloses a dual-network adhesive for crosslinking of transglutaminase and a preparation method of the dual-network adhesive. The preparation method comprises the following steps: simplytreating gelatin, mixing the gelatin with glucan aldehyde, and performing crosslinking under physiological conditions through the promotion action of the transglutaminase to form the dual-network adhesive. According to the double-network characteristic, the strength of the adhesive body is enhanced, and the adhesion performance of the adhesive is improved through introduction of the glucan aldehyde. The preparation method has the characteristics of simple raw material synthesis, injectable adhesive and easiness in application. By utilizing the characteristics, the dual-network adhesive can beused as a surgical sealant, a medical adhesive and the like to be clinically applied, and has a good application prospect in the fields of human body internal wound repair and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical tissue engineering materials, and more specifically relates to a transglutaminase-catalyzed cross-linked double-network adhesive and a preparation method thereof. Background technique [0002] At present, with the wide application of bioadhesives in the field of tissue engineering, higher requirements are put forward for the way of forming adhesives. From a biological point of view, the method of using enzymes to promote cross-linking to form adhesives is different from traditional There are many advantages over chemically cross-linked adhesives. For example, transglutaminase (Transglutaminase, hereinafter referred to as TGase) is a class of enzymes that catalyze acyl transfer reactions, which can make proteins through the introduction of amines, intra-molecular and intermolecular cross-linking, and deamination. The structure of the protein is changed so that the protein can form a high-molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/10A61L24/00
CPCA61L24/08A61L24/104A61L24/001A61L2400/04A61L2400/06C08L5/02C08L89/00
Inventor 赵瑾李阳原续波侯信
Owner TIANJIN UNIV
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