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Anti-tumor composition

A composition and tumor technology, applied in the direction of anti-tumor drugs, drug combinations, microorganisms, etc., can solve the problems of difficult to control tumor cells, eliminate cancer cells, cannot improve anti-tumor effects, etc., restore immune function, and enhance anti-cancer effects. Effect

Pending Publication Date: 2021-03-16
GENEMEDICINE CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Since the rapid proliferation and migration of cancer cells induced by the overexpression of C-met exceeds the removal rate of cancer cells in the immune system, it is difficult to eliminate cancer cells by immune response
Moreover, since the center of the immune response in the tumor microenvironment is heavily prone to suppression as tumor size increases, it is more difficult to control tumor cells with monotherapy and antitumor effects cannot be improved by antitumor immunotherapy

Method used

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  • Anti-tumor composition
  • Anti-tumor composition
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0151] Preparation of experiments and construction of recombinant vectors

[0152] [Preparation Example 1] Cell Acquisition and Culture

[0153] As the cell culture medium, Dulbecco's modified Eagle's medium (DMEM; kit, Grand Island, New York), Roseville Park Memorial medium (RPMI; kit) or a medium containing 10% fetal bovine serum, L-glucose Minimal essential medium (MEM; kit) of aminoamide (2 mmol / L), penicillin (100 IU / mL) and streptomycin (50 mg / mL). HEK293 (expressing adenovirus E1 site, human embryonic kidney cell line), H1975 (human non-small lung cancer cell line) and HaK (hamster renal carcinoma cell line) were purchased from the American Type Culture Collection (ATCC , VA). HaP-T1 (hamster pancreatic cancer cell line) was provided by Dr. Masato Abei (University of Tsukuba, Ibaraki, Japan). All cell lines were grown in a humidified environment at 37°C and 5% CO2 and tested negative for Mycoplasma using Hoeschst dye, cell culture, and P CR.E.coli (Escherichia coli...

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Abstract

The present invention relates to: an oncolytic adenovirus capable of co-expressing interleukin-12 and a C-met-inhibiting oligonucleotide; and an anti-tumor immune-boosting composition and an anti-cancer composition which comprise same. In the present invention, an adenovirus system having simultaneous effects of IL-12 expression and C-met inhibition during cancer gene treatment has been identifiedfor the first time, and an adenovirus system of the present invention simultaneously expresses interleukin-12 and inhibits C-met so as to restore immune functions in a tumor environment, thereby enhancing anti-cancer effects such as the inhibition of tumor recurrence and tumor growth and inhibiting tumor metastasis, and thus the present invention can be effectively used in the treatment of cancer.

Description

technical field [0001] The invention relates to a composition for anti-tumor or enhancing anti-tumor immunity, which comprises a recombinant adenovirus co-expressing interleukin 12 (IL-12) and a nucleic acid molecule inhibiting the expression of tyrosine kinase Met (C-met). Background technique [0002] Tumor immunotherapy is a method of inducing an immune response against tumors by enhancing the body's overall immune function and treating tumors through it. Research on tumor immunotherapy is actively underway. However, an immunosuppressive environment almost always develops during the developing stages of cancer, so even when the body's immune system is actively functioning, tumors cannot be easily removed. Tumor cells themselves express many abnormal antigens. These antigens elicit an eradication response through immune surveillance, allowing tumor cells to evade immune surveillance even when the body's immune system is active. In addition, this phenomenon has been foun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/86C12N15/113C07K14/54A61K38/20A61K48/00A61P35/00
CPCA61P35/00C12N15/86C12N2310/14C12N2310/20C12N15/1138C12N15/1137C07K14/5434C12N2710/10332C12N2710/10343A61K35/761C12N2810/405C12N2840/203C12N2800/40A61K48/005A61K38/00C07K14/71C12Y207/10001C12N2310/531C12N15/1136A61K48/00C12N2710/10041A61K38/208
Inventor 尹彩钰吴彦姝
Owner GENEMEDICINE CO LTD
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