Method for removing allergic components in rhizoma polygonati extract

An extract and allergy technology, which is applied in the field of removing allergic components in Polygonatum chinensis extract, can solve the problems of many residual allergenic substances and many operations, and achieve the effects of high selectivity, good repeatability and stable separation process.

Inactive Publication Date: 2021-03-19
浙大宁波理工学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many operations, and there are many residues of allergens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The dried Polygonatum tuber was crushed and passed through a 60-mesh sieve to obtain Polygonatum tuber powder, weighed 1000g, put it into an ultrasonic extraction kettle, added 8-10 L of ethanol, and ultrasonically extracted for 60 min under the condition of air-lift stirring at 50°C. The ultrasonic power was 120W, the ultrasonic frequency is 32kHz, it excites the ultrasonic sound, radially vibrates the cylindrical transducer, ultrasonically once every 2min, each ultrasonic 4min, the extract is filtered, concentrated under reduced pressure at 45℃ for 60min under 450Pa pressure, 20% ethanol-water solution Dissolve the extract to a concentration of 0.5g crude drug / mL, pass through D101 macroporous resin, 20% ethanol-water, 40% ethanol-water, 60% ethanol-water, 80% ethanol with a volume of 6Bv and a flow rate of 2Bv / h -Water, 100% ethanol solution elution, the eluents of each concentration were sampled separately, and the elution phases of 20% ethanol-water, 40% ethanol-wat...

Embodiment 2

[0013] The 1-month-old test rabbits were divided into 4 groups, each group of 10 rabbits, respectively with the Rhizoma Polygonatum extract group, the allergic component removal group, the allergic component group and the control group, the experimental animals were subcutaneously injected with 0.5 mg / ml extract 1ml, and the control group With 1ml 0.9% sodium chloride injection, IgE enzyme-linked immunoassay kit (ELISA) was used to measure the rabbit serum IgE level 2 hours after injection. ml, the allergic component group was 6.32±1.47U / ml, and the control group was 0.59±0.28U / ml.

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Abstract

The invention discloses a method for removing allergic components in rhizoma polygonati extract. Compared with an existing ''nine-time steaming and nine-time sunning''process for traditional Chinese medicine processing, the method for performing selective separation of the allergic components in the rhizoma polygonati extract by using the preparative chromatography has simple ultrasonic extractionoperation and high selectivity, a stable separation process and high repeatability, and the rhizoma polygonati extract from which the allergic components are removed cannot produce rabbit immunoglobulin E (IgE) mediated allergic reaction.

Description

technical field [0001] The invention relates to the extraction of Polygonatum, in particular to a method for removing allergic components in the Polygonatum extract. Background technique [0002] Polygonatum is the genus Polygonatum (Liliaceae) of Liliaceae (Liliaceae) Polygonatum ) Rhizomes of various plants, [Chinese Pharmacopoeia] (2000 edition) recorded Polygonatum ( Polygonatum sibiricun Red), Rhizoma Polygonatum ( Polygonatum cyrtonema hua), Yunnan Polygonatum ( Polygonatum kingianun Coll et hemsl). "Compendium of Materia Medica" records that "Huangjing, sweet and slightly pungent, warm in nature, nourishes the middle and nourishes Qi, treats five labors and seven injuries, helps muscles and bones, resists cold and heat, moistens the heart and lungs, fills the essence, and benefits the spleen and stomach." The extract of Rhizoma Polygonatum has a numbing taste, which can irritate the throat, cause numbness of the mouth and tongue, and cause allergic reactions. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61K125/00
CPCA61K36/8969A61K2236/33A61K2236/51A61K2236/55
Inventor 王强危凤阮晓杨丽
Owner 浙大宁波理工学院
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