Unlock instant, AI-driven research and patent intelligence for your innovation.

A dual-channel fluorescent probe for detecting viscosity and hydrogen peroxide and its preparation and application

A hydrogen peroxide, fluorescent probe technology, applied in the field of fluorescent probes, can solve problems such as limitations, achieve the effects of improving sensitivity, simple detection means, and avoiding spectral interference

Active Publication Date: 2021-08-24
SHANXI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limited disease models limit our understanding of viscosity and H 2 o 2 In-depth understanding of the internal relationship between blood levels and related diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A dual-channel fluorescent probe for detecting viscosity and hydrogen peroxide and its preparation and application
  • A dual-channel fluorescent probe for detecting viscosity and hydrogen peroxide and its preparation and application
  • A dual-channel fluorescent probe for detecting viscosity and hydrogen peroxide and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation and characterization of a fluorescent probe for dual-channel detection of viscosity and hydrogen peroxide:

[0053]

[0054] (1) 4-bromo-N, N-diphenylaniline (810mg, 2.5mmol) and Pd(dppf)Cl 2 (183mg, 0.25mmol) was dissolved in anhydrous THF (35mL), then added K 2 CO 3 / H 2 O (2.07g, 15mmol / 25mL). The reaction mixture was stirred at reflux under argon for 1 hour. Then (5-formylthiophen-2-yl)boronic acid (780 mg, 5.0 mmol) dissolved in anhydrous tetrahydrofuran (15 mL) was slowly added to the reaction solution, and the reaction mixture was stirred at 80° C. for 12 h. Cool to room temperature, concentrate the solvent in vacuo, and purify by silica gel column chromatography (petroleum ether / ethyl acetate, 50:1, v / v) to obtain a yellow solid, compound 2 (899 mg, yield 79%). 1 H NMR (400MHz, CDCl 3 ):δ9.85(s,1H),7.69(d,J=4.0Hz,1H),7.51(d,J=8.8Hz,2H),7.30–7.20(m,5H),7.17–7.11(m, 4H), 7.11–7.03 (m, 4H).

[0055] (2) t-BuOK (138mg, 1.23mmol) and mono(4-(((...

Embodiment 2

[0058] Aggregation-induced fluorescence emission characteristics of fluorescent probe Mito-TPB in ethanol / n-hexane mixed solvent

[0059] The fluorescent probe in embodiment 1 is diluted to final concentration with ethanol / n-hexane mixed solvent to be 5 μ mol / L, and fixed excitation wavelength is 480nm, and the fluorescence emission spectrum ( Figure 4 ), and draw the curve of probe fluorescence intensity changing with the volume content of n-hexane in ethanol / n-hexane mixed system ( Figure 5 ). As the volume content of n-hexane increases from 0% to 95%, the fluorescence intensity at 695nm gradually increases and blue-shifts to 655nm, and reaches the maximum when the volume content of n-hexane is 90%, indicating that the probe has typical aggregation-induced luminescence characteristics .

Embodiment 3

[0061] Fluorescent Response Characteristics of Fluorescent Probe Mito-TPB to Viscosity in Methanol / Glycerol Mixed Solvent

[0062] Dilute the probe in Example 1 to a final concentration of 5 μmol / L with methanol / glycerin mixed solvent, and the fixed excitation wavelength is 488nm, and record the fluorescence emission spectrum ( Figure 6 ), and plot the fluorescence intensity value of the probe at 666nm (log I 666 ), a linear relationship diagram ( Figure 7 ). As the volume ratio of glycerol increases from 0% (0.59cP) to 99% (950cP), the near-infrared fluorescence emission at 666nm increases sequentially, indicating that the probe can achieve highly sensitive detection of environmental viscosity.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a dual-channel fluorescent probe Mito-TPB for detecting viscosity and hydrogen peroxide, its preparation and application, and belongs to the technical field of fluorescent probes. A dual-channel fluorescent probe Mito-TPB for detecting viscosity and hydrogen peroxide provided by the present invention has aggregation-induced luminescent properties, and can be used as a detection reagent to simultaneously detect viscosity and H in inflammatory cell models or tumor tissue models. 2 o 2 The detection method is simple and sensitive.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a dual-channel fluorescent probe for detecting viscosity and hydrogen peroxide, as well as its preparation and application. Background technique [0002] Cell viscosity, as an important microenvironmental parameter, plays a crucial role in many diffusion-mediated biological processes, such as signal transport and electron transport, etc. Mitochondria are considered to be the energy factories of cells and are closely related to cell vitality and physiological activities. Abnormal changes in viscosity can lead to mutations in the mitochondrial network organization, which in turn affects the diffusion of mitochondrial metabolites, eventually leading to inflammation, diabetes, neurodegenerative diseases, and even cancer. [0003] Hydrogen peroxide (H 2 o 2 ) is a physiologically active oxygen molecule mainly produced in mitochondria, which plays a regulatory...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07F5/02C09K11/06G01N21/64
CPCC07F5/025C09K11/06C09K2211/1014C09K2211/1029C09K2211/1092G01N21/6428G01N21/643G01N2021/6432
Inventor 樊丽暂琪张跃伟
Owner SHANXI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com