Unlock instant, AI-driven research and patent intelligence for your innovation.

Fluorescent viscosity probe for monitoring mitochondrial autophagy as well as preparation and application thereof

A technology for mitophagy and mitochondria, which is applied in luminescent materials, measurement devices, chemical instruments and methods, etc., can solve the problems of unfavorable mitochondrial labeling and imaging, probe detachment, and influence on the effectiveness of luminescent materials, and achieves good application prospects, Simple detection method

Pending Publication Date: 2021-02-05
SHANXI UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many mitochondria-targeted viscous fluorescent probes have been reported in the literature, but the highly sensitive viscous probes that can really be used to monitor the process of mitophagy are extremely limited, and most of these probes only bind to the mitochondrial membrane potential through electrostatic interaction , that is, when the mitochondrial membrane potential decreases, the electrostatic attraction decreases or even disappears, and the probe will detach from the mitochondrial matrix, which is extremely unfavorable for mitochondrial labeling and imaging
In addition, most of these probes are based on aggregation-induced quenching luminescence mechanism, that is, low-concentration luminescence, high-concentration or aggregation state will cause fluorescence quenching, which seriously affects their effectiveness as luminescent materials.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent viscosity probe for monitoring mitochondrial autophagy as well as preparation and application thereof
  • Fluorescent viscosity probe for monitoring mitochondrial autophagy as well as preparation and application thereof
  • Fluorescent viscosity probe for monitoring mitochondrial autophagy as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation and characterization of a fluorescent viscosity probe for monitoring mitophagy:

[0048]

[0049] (1) In a round bottom flask, 4-bromophenylacetonitrile (0.588g, 3mmol) and t-BuOK (0.336g, 3mmol) were successively added to (30mL) absolute ethanol, and stirred at room temperature for 10 minutes; then 4-Diethylamino-2-methoxy-benzaldehyde (0.621g, 3mmol) was slowly added to the above mixture, and refluxed for 6 hours; the system was cooled to room temperature, the solvent was concentrated in vacuo, and purified by silica gel column chromatography (petroleum ether / ethyl acetate, 5:1, v / v) to obtain compound 2 (0.806 g, yield 70%) as a yellow solid. 1 H NMR (400MHz, CDCl 3 ):δ(ppm):8.26(d,J=8.8Hz,1H),7.91(s,1H),7.53–7.48(m,4H),6.36(dd,J=9.2,2.0Hz,1H),6.11 (s, 1H), 3.87 (s, 3H), 3.43 (q, J=6.8Hz, 4H), 1.23 (t, J=7.2Hz, 6H).

[0050] (2) Compound 2 (0.346g, 0.9mmol), K 2 CO 3 (0.138g, 1mmol) and 4-pyridylboronic acid (0.123g, 1mmol) were mixed and dissolve...

Embodiment 2

[0053] Aggregation-induced fluorescence emission characteristics of fluorescent probe CS-Py-BC in acetonitrile-water mixed solvent

[0054] With acetonitrile-water mixed solvent, the fluorescent probe in Example 1 is diluted to a final concentration of 5 μmol / L, and the fixed excitation wavelength is 470 nm, and the fluorescence emission spectrum ( Figure 4 ), and plot the probe relative fluorescence intensity (I / I 0 ) in the acetonitrile-water mixed system with the curve of volume content change ( Figure 5 ). As the water volume ratio increased from 0% to 95%, the fluorescence intensity at 686nm gradually increased and blue-shifted to 645nm, and reached the maximum when the water volume was 85%, indicating that the probe had typical aggregation-induced luminescence characteristics.

Embodiment 3

[0056] Fluorescence Response Characteristics of Fluorescent Probe CS-Py-BC to Viscosity in Water-Glycerol Mixed Solvent

[0057] Fluorescent probe in embodiment 1 is diluted to final concentration with water-glycerol mixed solvent to be 5 μ mol / L, and fixed excitation wavelength is 470nm, and the fluorescence emission spectrum ( Figure 6 ), and plot the fluorescence intensity value of the probe at 650nm (log I 650 ), the curve ( Figure 7 ). As the glycerol volume ratio increases from 0% (0.903cP) to 100% (1000cP), the fluorescence intensity at 650nm increases sequentially, and reaches the maximum value when the glycerol volume is 100%, indicating that the relative fluorescence intensity of the probe increases with the viscosity of the environment. increased significantly.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fluorescent viscosity probe CS-Py-BC for monitoring mitochondrial autophagy, and belongs to the technical field of viscosity fluorescent probes. The fluorescent viscosity probe CS-Py-BC for monitoring mitochondrial autophagy provided by the invention has aggregation-induced emission characteristics, can be fixed in mitochondria through covalent bonds, and is used as a detection reagent for monitoring the viscosity change of mitochondria in the mitochondrial autophagy process, and the detection means is simple and sensitive.

Description

technical field [0001] The invention belongs to the technical field of viscosity fluorescent probes, and in particular relates to a fluorescent viscosity probe for monitoring mitophagy and its preparation and application. Background technique [0002] As an important organelle of eukaryotic cells, mitochondria are considered to be the energy factory of cells, regulating cell energy metabolism, signal transduction and cell differentiation, growth and death. Mitochondrial damage or dysfunction can lead to many pathological processes, such as aging, apoptosis and cell damage, etc. In order to maintain the stability of mitochondrial quality and quantity, cells can selectively decompose and reuse damaged or excess mitochondria through autophagy. Mitophagy is a type of autophagy, that is, damaged mitochondria are selectively sequestered into autophagic mitochondria, and then autophagic mitochondria fuse with nearby lysosomes to form autolysosomes (ie, autophagosomes that wrap mit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/57C09K11/06G01N11/00
CPCC07D213/57C09K11/06C09K2211/1007C09K2211/1029G01N11/00G01N2011/008
Inventor 王晓东樊丽张跃伟李峰董川
Owner SHANXI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com