Preparation and Application of Alkaline Phosphatase Fluorescent Probe Based on Quinoline-Xanthene
A fluorescent probe and phosphatase technology, applied in the field of fluorescent probes, can solve problems such as detection of ALP
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Synthesis of Fluorescent Probes
[0027] Synthetic routes such as figure 1 . in N 2 Under protection, QX-OH (190 mg, 0.5 mmol), phosphorus oxychloride (230 mg, 1.5 mmol), and pyridine (119 mg, 1.5 mmol) were added to a 25 mL round-bottomed flask, followed by 6 mL of CH 2 Cl 2 dissolve it. After the reaction was stirred at room temperature for 4 hours, the resulting mixture was poured into ice and stirring continued overnight. After the reaction was completed, the solvent was removed under reduced pressure, and the resulting crude product was purified by column chromatography (CH 2 Cl 2 / CH 3 OH=2:1) to obtain a dark blue solid product QX-P (150 mg, yield 65%), which is the fluorescent probe. 1 H NMR(400MHz, DMSO)δ8.66(d,J=9.4Hz,1H),8.56(t,J=10.8Hz,2H),8.34(d,J=9.3Hz,1H),8.22(d,J =7.7Hz,1H),8.07-7.97(m,1H),7.80-7.75(m,1H),7.25(d,J=8.4Hz,1H),7.07(s,1H),6.92(s,1H) ,6.71–6.66(m,2H),4.93–4.88(m,2H),2.64(t,J=6.2Hz,4H),1.84–1.79(m,2H),1.52(t,J=6.9Hz,3H) ). 13 CNM...
Embodiment 2
[0029] Fluorescent probe and ALP solution preparation
[0030] Preparation of probe solution: Weigh a certain amount of probe and dissolve it in dimethyl sulfoxide to make 1×10 -4 Stock solution for M. Add 1.0 mL of the probe's stock solution to a 10 mL volumetric flask, and make up to the volume with Tris-HCl buffer solution to obtain a concentration of 1.0 × 10 -5 mol / L of fluorescent probe solution. ALP was formulated to the following concentrations (0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 U / mL).
Embodiment 3
[0032] Determination of the fluorescence spectrum of the interaction between fluorescent probes and ALP
[0033] figure 2 is the fluorescence spectrum of the interaction between the fluorescent probe and ALP, the concentration of the fluorescent probe is 10 μM, and the concentration of ALP is 0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 U / mL. . The excitation wavelength used in the experiment was 717 nm, and the emission wavelength ranged from 730 to 900 nm. The width of the slit is 10.0 nm / 10.0 nm, and the fluorescence measuring instrument used is a Hitachi F4600 fluorescence spectrophotometer. from figure 2 It can be seen that before the addition of ALP, the probe itself has almost no emission peak due to the quenching effect of phosphate; with the addition of ALP, the emission peak at 770 nm is greatly enhanced, and with the increase of ALP concentration, the probe itself has almost no emission peak. The fluorescence intensity of the needles increased con...
PUM
| Property | Measurement | Unit |
|---|---|---|
| width | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com