Human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and preparation method thereof

A technology of mesenchymal stem cells and extracellular matrix, applied in the fields of pharmaceutical formula, medical science, prosthesis, etc., can solve the problems of poor mechanical strength, achieve the effect of improving mechanical strength, good biocompatibility, and preventing cell loss

Active Publication Date: 2021-03-23
湖南美柏生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing human collagen hydroge

Method used

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  • Human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and preparation method thereof
  • Human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and preparation method thereof
  • Human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and preparation method thereof

Examples

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Example Embodiment

[0036] According to a typical embodiment of the present invention, there is provided a method for preparing a hydrogel based on a human mixed collagen extracellular matrix source composite collagen, wherein the method comprises:

[0037] The human hemolinalized stem cell extracellular matrix source composite collagen extract is dissolved in a buffer solution to obtain a protein solution having a mass concentration of 0 to 100 mg / ml;

[0038] The protein solution was mixed with natural molecules and artificially mixed, and the mixed solution was obtained; the use of the artificially combined molecule was 0 to 100 mg / ml; the native molecule used end concentration of 0 to 100 mg / ml;

[0039] The mixed solution was added to the crosslinking activator to mix the crosslinking reaction, and the crosslinking reaction liquid was obtained; the terminal concentration of the crosslinking activator was 1 to 30 mg / mL;

[0040] The cross-linking reaction solution was purified to obtain a...

Example Embodiment

[0075] Example 1

[0076] Step S1, formulated 100 mm MES buffer solution and adjusted pH to 6-7 with 1 M NaOH.

[0077] Step S2, adding a human supramine sub-extract of human hemmelal stem cell extraction, enabling the mass concentrated mass concentration of human mixed stem cell extracellular matrix source in the mixed solution to 50 mg / ml, stir well;

[0078] Step S3, EDC was added, and the mass concentration of activator in the mixed solution was 1 mg / ml to stir well, and at room temperature for 4-6 h, the hydrogel ECM 50-EDC 1 was obtained.

Example Embodiment

[0079] Example 2

[0080] Step S1, formulated 100 mm MES buffer solution and adjusted pH to 6-7 with 1 M NaOH.

[0081] Step S2, adding a human supramine sub-extract of human hemmelal stem cell extraction, enabling the mass concentrated mass concentration of human mixed stem cell extracellular matrix source in the mixed solution to 50 mg / ml, stir well;

[0082] Step S3, the EDC was added, allowing the mass concentration of activator in the mixed solution to 3 mg / ml, stirred well, and at room temperature for 4-6 h, resulting in hydrogel ECM 50-EDC 3.

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Abstract

The embodiment of the invention discloses a human mesenchymal stem cell-based extracellular matrix-derived composite collagen extracting solution hydrogel and a preparation method thereof. The methodcomprises the following steps that a human mesenchymal stem cell extracellular matrix-derived composite collagen extracting solution is dissolved into a buffer solution to obtain a protein solution with the mass concentration of 0.1-100 mg/mL; natural molecules and artificially synthesized molecules are added into the protein solution, and uniform mixing is conducted to obtain a mixed solution, wherein the use final concentration of the artificially synthesized molecules is 0-100 mg/mL, and the use final concentration of the natural molecules is 0-100 mg/mL; a cross-linking activating agent isadded into the mixed solution, and uniform mixing is conducted to obtain a cross-linking reaction solution, wherein the use final concentration of the cross-linking activating agent is 1-30 mg/mL; and the cross-linking reaction liquid is purified to obtain the composite collagen extracting solution hydrogel. According to the hydrogel and the method, the gelling time is short, the biocompatibilityis good, the stability is good, and the injectability is achieved.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of medical biomaterials, in particular to a hydrogel based on human mesenchymal stem cell extracellular matrix derived composite collagen extract and a preparation method thereof. Background technique [0002] Hydrogel has a highly hydrophilic three-dimensional network structure and can maintain a high water content (70-90%). In addition, hydrogels not only provide structural support for cell attachment, but also provide a variety of biochemical and biophysical factors to regulate cell behavior. Injectable hydrogels are one of the recent focuses, and by being applied directly on the desired site in a minimally invasive manner, possible infection risks during surgery can be minimized with less scarring and / or pain, and allow Clinical application is performed in an outpatient setting rather than an operating room, thereby minimizing healthcare costs while maximizing availability. Fu...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/24A61L27/20A61L27/18A61L27/50A61L27/52A61L27/58
CPCA61L27/3834A61L27/3839A61L27/24A61L27/20A61L27/18A61L27/50A61L27/52A61L27/58A61L2400/06A61L2430/34C08L5/08C08L5/04C08L71/02
Inventor 魏强肖锷
Owner 湖南美柏生物医药有限公司
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