Method for extracting soybean protein isolate from soybean whey wastewater

A technology for soybean whey wastewater and soybean protein isolate, which is used in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc.

Pending Publication Date: 2021-03-26
HEZE BRANCH QILU UNIV OF TECH(SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for extracting soybean protein isolate from soybean whey wastewater to solve the defects in the prior art

Method used

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  • Method for extracting soybean protein isolate from soybean whey wastewater
  • Method for extracting soybean protein isolate from soybean whey wastewater
  • Method for extracting soybean protein isolate from soybean whey wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: pH adjustment: Take 41.9 kg of soybean whey wastewater generated during the production of soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.0, and obtain soybean whey wastewater after adjusting the pH value;

[0033] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 70° C. with a heat exchanger;

[0034] Step 3: Membrane filtration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.2 bar, the working temperature is 70°C, and the transmembrane pressure The difference (TMP) is 1.5bar, and the flux of the ceramic membrane is 312L / (m 2 * h), to obtain the intercepted concentrated solution;

[0035] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey protein...

Embodiment 2

[0038] Step 1: pH adjustment: Take 125 kg of soybean whey wastewater generated during the production of soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.5, and obtain soybean whey wastewater after adjusting the pH value;

[0039] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 75° C. with a heat exchanger;

[0040] Step 3: Microfiltration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.5 bar, the working temperature is 75°C, and the transmembrane pressure The difference (TMP) is 1.8bar, and the flux of the ceramic membrane is 261L / (m 2 * h), to obtain the intercepted concentrated solution;

[0041] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey protein, oli...

Embodiment 3

[0044] Step 1: pH adjustment: Take 441.76 kg of soybean whey wastewater in the process of extracting soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.0, and obtain the soybean whey wastewater after adjusting the pH value;

[0045] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 80° C. with a heat exchanger;

[0046] Step 3: Microfiltration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.4bar, the working temperature is 80°C, and the transmembrane pressure The difference (TMP) is 1.6bar, and the maximum flux of the ceramic membrane is 350L / (m 2 *h), to obtain the intercepted concentrated solution;

[0047] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey prote...

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Abstract

The invention discloses a method for extracting soybean protein isolate from soybean whey wastewater. The method for extracting the soybean protein isolate from the soybean whey wastewater comprises the following steps: step one, regulating the pH value of the soybean whey wastewater to 6-8.5 to obtain soybean whey wastewater with regulated pH value; step two, heating the soybean whey wastewater with regulated pH value obtained in the step one to 65-85 DEG C; step three, separating and concentrating the heated soybean whey wastewater obtained in the step two through a 0.1 mu m ceramic membraneseparation system to obtain an intercepted concentrate; step four, adding purified water into the intercepted concentrate obtained in the step three, and carrying out dialyzing so as to obtain a dialyzed concentrate; and step five, sterilizing the dialyzed concentrate solution obtained in the step four, and carrying out drying so as to obtain the soybean protein isolate powder. By using the the ceramic membrane with the pore diameter of 0.1 mu m, the method can concentrate the soybean whey wastewater to 20-50 times at a high flux no lower than 200 L/(m< 2 >* h), and intercept 100% of the soybean protein isolate in the soybean whey wastewater, thereby increasing the content of the soybean protein isolate in the extract by 48% or above.

Description

technical field [0001] The invention belongs to the field of soybean whey wastewater treatment, in particular to a method for extracting soybean protein isolate from soybean whey wastewater. Background technique [0002] Soybean whey wastewater refers to the wastewater generated when soybean protein isolate is produced from low-temperature defatted soybean meal by alkali-dissolving and acid-precipitating method. The specific production process is that the low-temperature defatted soybean meal is homogenized and added with water to adjust the pH to be alkaline, the soybean protein isolate in the soybean meal is dissolved in the lye, and the supernatant is obtained through centrifugation, and then the pH of the supernatant is adjusted to be acidic. Centrifugation precipitates the soy protein isolate. For every 1t of soybean protein isolate produced, 30-50t of soybean whey wastewater will be produced. At present, the production capacity of soybean protein isolate in China is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34
CPCC07K1/145C07K1/34
Inventor 袁文鹏魏永峰许萍周田田候瑾
Owner HEZE BRANCH QILU UNIV OF TECH(SHANDONG ACAD OF SCI
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