Application of reagent for detecting content of calprotectin in faeces in preparation of uterine lesion screening kit

A calprotectin and kit technology, which can be used in measurement devices, biological tests, disease diagnosis, etc., can solve problems such as no uterine lesions and calprotectin, and achieve non-invasive screening and good application prospects.

Active Publication Date: 2021-03-30
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no relationship between uterine lesions and calprotectin, let alone the relationship between uterine lesions and calprotectin in feces

Method used

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  • Application of reagent for detecting content of calprotectin in faeces in preparation of uterine lesion screening kit
  • Application of reagent for detecting content of calprotectin in faeces in preparation of uterine lesion screening kit
  • Application of reagent for detecting content of calprotectin in faeces in preparation of uterine lesion screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Screening kit (ELISA) of the present invention

[0027] 1. Composition

[0028] (1) Pre-coated plate: a ninety-six-well plate in which the inner wall and bottom of the wells are coated with anti-human calprotectin rabbit IgG antibody.

[0029] (2) Enzyme-labeled antibody: (30 times concentrated) HRP-labeled anti-human calprotectin rabbit IgG antibody.

[0030] (3) Standard product: calprotectin.

[0031] (4) Buffer: PBS containing 1% BSA, 0.05% Tween 20.

[0032] (5) Chromogen: TMB substrate solution.

[0033] (6) Termination solution: 1N sulfuric acid.

[0034] 2. Sample Preparation

[0035] (1) Collect specimens with a special adherent for sample sampling, place the stool in the center of the unfolded adherent, and pay attention that the stool should not be stained with urine, blood, toilet water, toilet paper and other pollutants;

[0036] (2) Use the sampling stick to insert the stool sample, then put the sampling stick back into the stool samplin...

Embodiment 2

[0050] Example 2 Relationship between uterine lesions and content of fecal calprotectin (FC)

[0051] From June 2019 to September 2019, the inventor performed fecal calprotectin detection on some infertile patients who visited the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine (using the calprotectin detection kit (colloidal gold method) of Xiamen Weizheng Biotechnology Co., Ltd. ), including 50 patients with uterine lesions (16 cases of uterine fibroids, 10 cases of endometrial polyps, 11 cases of thin endometrium in the middle luteal body (referred to as "thin endometrium"), and poor blood supply of the endometrium in the middle corpus luteum (referred to as "endometrium") membrane blood supply") in 2 cases, intrauterine adhesions in 5 cases, and adenomyosis in 6 cases). At the same time, healthy adult females were used as controls.

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Abstract

The invention provides application of a reagent for detecting the content of calprotectin (faecal calprotectin) in faeces in preparation of a uterine lesion screening kit, and belongs to the field ofdisease detection kits. By detecting the content of calprotectin in feces of different people, it is found that the content is remarkably associated with uterine lesions, a kit is developed with the help of the principle, uterine lesions can be rapidly screened in a non-invasive mode, and the application prospect is good.

Description

technical field [0001] The invention belongs to the field of disease screening kits. Background technique [0002] Calprotectin was first isolated from neutrophils by Fagerhol in 1980 and named L1 protein. It was later named after the discovery that its structure contained calcium and had antimicrobial properties. It has a history of 39 years. In 1988, Wilkinson et al. named it calgranulin based on this antigenic feature. Dorin and Freemont et al. further confirmed that these proteins have the structural features of S-100 protein, and later named this protective calcium-binding protein with multiple functions for calprotectin. [0003] Calprotectin is a heterodimeric complex composed of S100A8 and S100A9 in the S100 calcium-binding protein family, a calcium-zinc binding protein derived from neutrophils and macrophages, which can be detected in serum, body fluids or feces, Has been identified as a marker of inflammatory bowel disease IBD, and elevated levels of calprotectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/36G01N2333/4727G01N33/68G01N33/577
Inventor 陆华王通刘芊辰廖睿
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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