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Rapid screening method of ionizing radiation resistant microorganisms

A screening method and anti-ionization technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of safety requirements, inability to carry out experiments, etc., achieve low requirements, good promotion and use value, and good screening results Effect

Inactive Publication Date: 2021-04-02
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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Problems solved by technology

However, carrying out ionizing radiation experiments requires specialized equipment and relatively high safety requirements, and many laboratories are unable to carry out related experiments due to this limitation

Method used

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  • Rapid screening method of ionizing radiation resistant microorganisms
  • Rapid screening method of ionizing radiation resistant microorganisms
  • Rapid screening method of ionizing radiation resistant microorganisms

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Embodiment

[0024] 1. Determination of the survival rate of model strains in different concentrations of hydrogen peroxide:

[0025] (1) Resuscitate the frozen strain, inoculate the resuscitated strain into a solid medium that matches the strain for 24-72 hours, and take some single colonies and culture it in 20 mL liquid medium that matches the strain until In the logarithmic phase, pipette 100 μL of the bacterial solution into 20 mL of the same liquid medium, and shake the flask for 16-48 hours to reach the logarithmic growth phase.

[0026] (2) Take 1mL of the bacterial solution in the logarithmic growth phase and measure the OD 600 After the value, the bacterial solution was diluted to OD 600 = 1; the concentration is OD 600 = 1 part of the bacterial solution was diluted serially with a triangular coating stick (soaked in isopropanol and sterilized by fire), and spread on the plate for culture, as the control group without oxidative stress.

[0027] (3) Dilute 30% hydrogen peroxi...

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Abstract

The invention relates to a rapid screening method of ionizing radiation resistant microorganisms. The method comprises the following steps: firstly, activating a strain to be detected and carrying outliquid shake-flask culture; then carrying out culturing in a liquid culture medium until the early and middle periods of a logarithmic phase, and preparing bacterium suspension with OD600= 1; then dividing the bacterium suspension into two parts, wherein one part is used as a control group and is subjected to gradient dilution by adopting the liquid culture medium, after the concentration of bacterium liquid is diluted to 10000 times, the bacterium liquid is cultured, the other part is used as a treatment group and is mixed with hydrogen peroxide with the concentration of 3 mM, the mixture issubjected to constant-temperature shaking culture treatment to obtain treated bacterium liquid, the treated bacterium liquid is subjected to gradient dilution by adopting the liquid culture medium, and after the concentration of the bacterium liquid is diluted to 10000 times, the bacterium liquid is cultured; and finally, counting the quantity of bacterial colonies of the control group and the quantity of bacterial colonies of the treatment group respectively and calculating the survival rate; and if the survival rate of the treatment group is higher than that of the control group, determining that the strain to be detected has relatively high ionizing radiation resistant capability. The rapid screening method is simple to operate and high in efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid screening method for anti-ionizing radiation microorganisms. Background technique [0002] Ionizing radiation refers to rays with short wavelength, high frequency, and high energy, which can directly or indirectly ionize the atoms and molecules of matter through the interaction with matter. In terms of sources, the ionizing radiation that humans mainly receive includes natural radiation and artificial radiation. Among them, natural radiation mainly comes from the sun, cosmic rays and radionuclides existing in the earth's crust; artificial radiation mainly comes from medical equipment (such as medical and imaging equipment), research and teaching institutions, nuclear reactors and their auxiliary facilities, etc. In terms of types, ionizing radiation is divided into electromagnetic radiation (electromagnetic waves such as X-rays and gamma rays) and particle radiation (includi...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 章高森刘阳张威陈拓刘光琇
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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