A paper-based detection method for exosomes

A detection method, exosome technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of low requirements, enhanced visual signal, and easy detection and operation
CN112608983BActive Publication Date: 2021-06-11北京红果源生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京红果源生物技术有限公司
Publication Date
2021-06-11

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Abstract

The present invention provides a paper-based detection method for exosomes, including: (1) recognition and molecular conversion system, (2) amplification reaction system, and (3) LFB detection and analysis system. The present invention converts exosomes into single-stranded DNA targets, amplifies the single-stranded DNA through nucleic acid amplification technology, and further utilizes PtAu-NiO 2 The amplified single-stranded DNA is detected by the lateral flow chromatography test paper with the nanozyme as the signal probe. The invention uses nucleic acid lateral flow chromatography test paper as the terminal detection platform, solves the problem that exosomes cannot be detected qualitatively and quantitatively at the same time, and realizes simple and low-cost detection of exosomes.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a paper-based detection method for exosomes. Background technique

[0002] The nanoscale particle size and ultra-trace concentration of exosomes in body fluids pose great challenges for their identification and analysis. Existing analysis methods not only rely on large and expensive instruments, but also require complex pretreatment processes and professional operators. In addition, traditional exosome detection methods cannot be qualitative and quantitative at the same time. Therefore, the development of fast, simple, low-cost, and highly sensitive exosome detection methods is the key to the rapid development and popularization of clinical disease diagnosis and treatment methods using exosomes as biomarkers.

[0003] Lateral Flow Chromatography (LFB) is a sensitive, cheap and simple visual rapid detection method. Early LFBs were based on immune recogni...

Claims

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