Preparation of spinal cord subacute combined degeneration disease model

A technology for subacute and degenerative diseases, applied in the field of medical biology, can solve the problems of insufficient vitamin B content, inability to complete vitamin B intake, and high cost of transgenic mice, reducing research costs, low prices, and increasing the speed of preparation. Effect

Active Publication Date: 2021-04-09
王涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing animal model of subacute combined degeneration of the spinal cord is prepared as follows: through manipulation at the genetic level, the mice cannot complete the vitamin B 12 intake, thus making vitamin B in mice 12 Subacute combined degeneration of the spi...

Method used

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  • Preparation of spinal cord subacute combined degeneration disease model
  • Preparation of spinal cord subacute combined degeneration disease model
  • Preparation of spinal cord subacute combined degeneration disease model

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: The preparation of the spinal cord subacute combined degenerative disease model, the method comprises the following steps:

[0026] S1. Dilute 10 μl of hexachlorobutadiene into 490ul of edible oil, which is corn oil in this embodiment;

[0027] S2. According to the dose of 50-250 μl / mouse, the mice are given intragastric or intraperitoneal injection, and the mice are normally fed during the medication period;

[0028] S3. Perform intragastric or intraperitoneal injection again at an interval of 48 hours, for a total of three administrations;

[0029] S4. Test the mice 24 hours after the third administration, and observe whether there are clinical symptoms of subacute combined degeneration of the spinal cord:

[0030] S4.1, the body weight of mice is tested and recorded every 24 hours during the medication period;

[0031] S4.2. Conduct behavioral evaluation on mice, including but not limited to: gait, pole climbing, rotarod and wire grid experiments;

...

Embodiment 2

[0033] Embodiment 2: Group A of test mice, a group of 10, carried out the following operations to it:

[0034] S1. Dilute 10 μl of hexachlorobutadiene into 490 μl of corn oil;

[0035] S2. According to the dose of 50 μl / mouse, the mice were given intragastric or intraperitoneal injection, and the mice were normally fed during the medication period;

[0036] S3. Perform intragastric or intraperitoneal injection again at an interval of 48 hours, for a total of three administrations;

[0037] S4. Test the mice 24 hours after the third administration, and observe whether there are clinical symptoms of subacute combined degeneration of the spinal cord:

[0038] S4.1, the body weight of mice is tested and recorded every 24 hours during the medication period;

[0039] S4.2. Conduct behavioral evaluation on mice, including but not limited to: gait, pole climbing, rotarod and wire grid experiments;

[0040] S4.3. Measurement of serum vitamin B in mice 12 content.

Embodiment 3

[0041] Embodiment 3: Group B of test mice, a group of 10, carried out the following operations to it:

[0042] S1. Dilute 10 μl of hexachlorobutadiene into 490 μl of corn oil;

[0043] S2. According to the dose of 150 μl / mouse, the mice were given intragastric or intraperitoneal injection, and the mice were fed normally during the medication period;

[0044] S3. Perform intragastric or intraperitoneal injection again at an interval of 48 hours, for a total of three administrations;

[0045] S4. Test the mice 24 hours after the third administration, and observe whether there are clinical symptoms of subacute combined degeneration of the spinal cord:

[0046] S4.1, the body weight of mice is tested and recorded every 24 hours during the medication period;

[0047] S4.2. Conduct behavioral evaluation on mice, including but not limited to: gait, pole climbing, rotarod and wire grid experiments;

[0048] S4.3. Measurement of serum vitamin B in mice 12 content.

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Abstract

The invention discloses preparation of a spinal cord subacute combined degeneration disease model. The preparation comprises the following steps of diluting 10 microliters of hexachlorobutadiene into 490 microliters of corn oil; conducting intragastric administration or intraperitoneal injection on each mouse according to the dosage of 50-250 microliters, and feeding the mouse normally in the medication period; conducting intragastric administration or intraperitoneal injection again after 48 hours, and conducting administration three times in total; testing the mouse and observing whether a clinical symptom of a spinal cord subacute combined degeneration disease occurs or not, wherein the following modes that the weight of the mouse is tested and recorded every 24 hours in the medication period, and behavioral evaluation is performed on the mouse, evaluation contents comprise but are not limited to gait, pole climbing, bar rotating and wire grid experiments, and the content of the vitamin B12 in the serum of the mouse is measured after medication are included. Through the arrangement, the preparation of the spinal cord subacute combined degeneration disease model can be completed within 6 days by using the hexachlorobutadiene, and the hexachlorobutadiene is low in selling price, so that a preparation manner disclosed by the invention has the advantages of high speed and low cost compared with a traditional preparation method.

Description

technical field [0001] The invention relates to the technical field of medical biology, in particular to the preparation of a spinal cord subacute combined degenerative disease model. Background technique [0002] Subacute combined degeneration of the spinal cord, referred to as subacute combined degeneration, is due to vitamin B 12 Impaired intake, absorption, binding, transport, or metabolism of B vitamins in the body 12 Diseases of central and peripheral nervous system degeneration caused by insufficient content. The lesions mainly involve the dorsal cord, lateral cord, and peripheral nerves. The clinical manifestations are deep sensory loss of the lower extremities, sensory ataxia, spastic paralysis, and peripheral neuropathy, often accompanied by clinical signs of anemia. [0003] In order to better study the subacute combined degeneration of the spinal cord, it is necessary to establish corresponding animal models. Animal disease models are mainly used in experiment...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61D7/00
CPCA01K67/02A61D7/00
Inventor 孙雅迪韩超徐岩黄金莎熊念王涛
Owner 王涛
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