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Preparation method of synbiotic

A technology of synbiotics and mulberry leaves, applied in the field of synbiotics, can solve the problems of high molecular weight and viscosity, non-degradable, low bioavailability, etc., and achieve the effect of fast growth rate, high activity, and simplified purification steps

Active Publication Date: 2021-04-09
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mulberry leaf polysaccharides have been reported to have biological activities such as regulating glucose and lipid metabolism, but due to the high molecular weight and viscosity of mulberry leaf polysaccharides, their bioavailability in the body is low. At the same time, studies have found that polysaccharides contain a large number of non-α-1,4 configurations The glycosidic bonds in the body cannot be completely degraded by human digestive juice, and may be degraded into small molecular functional oligosaccharides to regulate the intestinal flora and play a role

Method used

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  • Preparation method of synbiotic
  • Preparation method of synbiotic
  • Preparation method of synbiotic

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Add mulberry leaf powder to distilled water to obtain a 10% (w / v) solution, extract polysaccharides in water at pH 4.9 and 80°C for 8 hours, centrifuge at 5000×g for 5 minutes, take the supernatant, and concentrate to obtain Mulberry leaf polysaccharide solution;

[0042] Take 3% mulberry leaf polysaccharide solution, add 1% hemicellulase, the reaction temperature is 50°C, the reaction time is 8h, the reaction pH is 6.0, and after centrifugation at 8000×g for 5min, the supernatant is taken and concentrated to obtain mulberry leaf oligosaccharides crude reaction solution;

[0043] The mulberry leaf oligosaccharide crude reaction solution is removed by combining nanofiltration (200Da) and ultrafiltration (2000Da) membrane systems to remove monosaccharides and polysaccharides that have not been degraded by enzymes and have a molecular weight greater than 2000Da in the oligosaccharide crude reaction solution to obtain Preliminary purified oligosaccharide solution;

[0044...

Embodiment 2

[0048] Add mulberry leaf powder to distilled water to obtain a 12.5% ​​(w / v) solution, extract polysaccharides from water at pH 4.8 and 70°C for 7 hours, centrifuge at 5000×g for 5 minutes, take the supernatant, concentrate to obtain mulberry leaf polysaccharide solution;

[0049] Take 3.25% mulberry leaf polysaccharide solution, add 0.75% hemicellulase, the reaction temperature is 45°C, the reaction time is 7h, the reaction pH is 5.7, and after centrifugation at 8000×g for 5min, the supernatant is taken and concentrated to obtain mulberry leaf oligosaccharides crude reaction solution;

[0050] The mulberry leaf oligosaccharide crude reaction solution is removed by combining nanofiltration (200Da) and ultrafiltration (2000Da) membrane systems to remove monosaccharides and polysaccharides that have not been degraded by enzymes and have a molecular weight greater than 2000Da in the oligosaccharide crude reaction solution to obtain Preliminary purified oligosaccharide solution; ...

Embodiment 3

[0055] Add mulberry leaf powder to distilled water to obtain a 15% (w / v) solution, extract polysaccharides from water at pH 4.5 and 60°C for 6 hours, centrifuge at 5000×g for 5 minutes, take the supernatant, and concentrate to obtain Mulberry leaf polysaccharide solution;

[0056] Take 3.5% mulberry leaf polysaccharide solution, add 0.5% hemicellulase, the reaction temperature is 45°C, the reaction time is 6h, the reaction pH is 5.5, and the supernatant is taken by centrifugation at 8000×g for 5min, concentrated to obtain the crude mulberry leaf oligosaccharide The reaction solution;

[0057] The above-mentioned mulberry leaf oligosaccharide crude reaction solution is removed by combining nanofiltration (200Da) and ultrafiltration (2000Da) membrane systems to remove monosaccharides and polysaccharides that have not been degraded by enzymes and have a molecular weight greater than 2000Da in the oligosaccharide crude reaction solution, Obtain a preliminary purified oligosacchar...

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Abstract

The invention discloses a preparation method of synbiotic, which uses mulberry leaves as raw materials, then hydrolyzing with hemicellulase, carrying out nanofiltration, ultrafiltration and column chromatography purification to obtain mulberry leaf oligosaccharide, enriching intestinal flora with glycolipid metabolism regulation activity by using the mulberry leaf oligosaccharide as a carbon source, and carrying out separation to obtain the most obviously enriched strain; and finally, co-culturing the purified mulberry leaf oligosaccharide and the enriched strain obtained by separation to prepare the synbiotic rich in mulberry leaf oligosaccharide and strain. The preparation method of the synbiotic is simple, and the prepared synbiotic can effectively regulate and control the glycolipid metabolism activity.

Description

technical field [0001] The present invention relates to the technical field of synbiotics, and more specifically relates to a preparation method of synbiotics. Background technique [0002] Mulberry leaf polysaccharides have been reported to have biological activities such as regulating glucose and lipid metabolism, but due to the high molecular weight and viscosity of mulberry leaf polysaccharides, their bioavailability in the body is low. At the same time, studies have found that polysaccharides contain a large number of non-α-1,4 configurations The glycosidic bonds in the diet cannot be completely degraded by human digestive juices, and may be degraded into small molecular functional oligosaccharides to regulate intestinal flora and exert their effects. [0003] Functional oligosaccharides cannot be directly absorbed and utilized by the human body because they cannot be degraded by the human gastrointestinal digestive juice. Instead, they serve as prebiotics, energy subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14C12N1/20C07H3/02C07H1/06A23L33/21A23L33/135A23L2/52A23K20/163A23K10/18C12R1/225
CPCC12P19/02C12P19/14C12N1/20C07H3/02C07H1/06A23L33/21A23L33/135A23L2/52A23K20/163A23K10/18A23V2002/00A23V2400/173A23V2200/3202A23V2200/3204A23V2200/328A23V2200/30A23V2200/3262A23V2250/284
Inventor 胡腾根邹宇晓廖森泰黎尔纳刘凡沈维治王思远
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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