Method, reagent strip and kit for colloidal gold labeling of Salmonella typhi recombinant antigen
A technology of Salmonella typhi and recombinant antigen, which is applied in the field of gold immunity, can solve the problems of not being able to significantly improve the color intensity and sensitivity of products, and achieve the effects of improving color intensity and sensitivity, deepening color intensity, and improving sensitivity
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Embodiment 1
[0040] Embodiment 1: A kind of method for colloidal gold labeling Salmonella typhi recombinant antigen, labeling method comprises the following steps:
[0041] (1) Liquid preparation
[0042] Chlorauric acid solution: Dissolve 10 g of chloroauric acid in ultrapure water, and after it is completely dissolved, dilute to 1000 mL, and store in the dark at 4°C for later use.
[0043] Trisodium citrate solution: Weigh 1 g of trisodium citrate, dissolve it in 100 mL of ultrapure water at room temperature, filter through a 0.22 μm membrane filter, and store at 4°C for later use.
[0044] Potassium stannate solution: Weigh 2 g of potassium stannate, dissolve it in 100 mL of ultrapure water at room temperature, filter it with a 0.22 μm filter membrane, and store it at 4°C for later use.
[0045] BSA solution: Weigh 10g of BSA, dissolve in 100mL of ultrapure water at room temperature, store at 4°C, and prepare immediately after use.
[0046] PEG20000 solution: Weigh 10g of PEG20000, di...
Embodiment 2
[0052] Embodiment 2: A kind of method for colloidal gold labeling Salmonella typhi recombinant antigen, labeling method comprises the following steps:
[0053] (1) Liquid preparation
[0054] Chlorauric acid solution: Dissolve 10 g of chloroauric acid in ultrapure water, and after it is completely dissolved, dilute to 1000 mL, and store in the dark at 4°C for later use.
[0055]Trisodium citrate solution: Weigh 1 g of trisodium citrate, dissolve it in 100 mL of ultrapure water at room temperature, filter through a 0.22 μm membrane filter, and store at 4°C for later use.
[0056] Potassium stannate solution: Weigh 2 g of potassium stannate, dissolve it in 100 mL of ultrapure water at room temperature, filter it with a 0.22 μm filter membrane, and store it at 4°C for later use.
[0057] BSA solution: Weigh 10g of BSA, dissolve in 100mL of ultrapure water at room temperature, store at 4°C, and prepare immediately after use.
[0058] PEG20000 solution: Weigh 10g of PEG20000, dis...
Embodiment 3
[0064] Embodiment 3: a kind of method for colloidal gold labeling Salmonella typhi recombinant antigen, labeling method comprises the following steps:
[0065] (1) Liquid preparation
[0066] Chlorauric acid solution: Dissolve 10 g of chloroauric acid in ultrapure water, and after it is completely dissolved, dilute to 1000 mL, and store in the dark at 4°C for later use.
[0067] Trisodium citrate solution: Weigh 1 g of trisodium citrate, dissolve it in 100 mL of ultrapure water at room temperature, filter through a 0.22 μm membrane filter, and store at 4°C for later use.
[0068] Potassium stannate solution: Weigh 2 g of potassium stannate, dissolve it in 100 mL of ultrapure water at room temperature, filter it with a 0.22 μm filter membrane, and store it at 4°C for later use.
[0069] BSA solution: Weigh 10g of BSA, dissolve in 100mL of ultrapure water at room temperature, store at 4°C, and prepare immediately after use.
[0070] PEG20000 solution: Weigh 10g of PEG20000, di...
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