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Method and kit for detecting new coronavirus IgG/IgM total antibody

A kit and total antibody technology, applied in the field of biomedicine, can solve the problems of high false positive and low detection efficiency in the detection of serum stock solution of healthy people, and achieve the effect of shortening the screening time

Inactive Publication Date: 2021-04-09
WUHAN AIBO TAIKE BIOTECH CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kit for detecting IgG / IgM total antibodies of the new coronavirus, so as to overcome the defects of low detection efficiency in the existing kits and high false positives in the detection of healthy human serum stock solution.

Method used

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  • Method and kit for detecting new coronavirus IgG/IgM total antibody
  • Method and kit for detecting new coronavirus IgG/IgM total antibody
  • Method and kit for detecting new coronavirus IgG/IgM total antibody

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Embodiment

[0029] Example Novel Coronavirus IgG / IgM Total Antibody Kit Product Double Antigen Sandwich Method Establishment and Detection

[0030] 1.1 Verification of coating protein activity

[0031] The new crown RBD protein can bind to its receptor ACE2 when it is active. When testing the activity of the coating protein, we selected 4 different batches of proteins developed by ABclonal (article numbers are RP01258, RP01278, RP01282, and the numbers are RP01258_9603040401, RP01258_9601050101 (two different batches of RP01258), RP01278_96010560901, RP012801)6 Conduct an activity test to select the batch with the best activity for the development of the double-antigen sandwich method of the new coronavirus IgG / IgM total antibody kit product. The specific method is as follows:

[0032] 1) Coat the recombinant ACE2 protein on a microwell plate at a concentration of 2-10ug / ml, overnight at 4°C.

[0033] 2) Shake off the liquid in the well, and use 2% BSA to block at 37° C. for 2 hours.

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Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a method and a kit for detecting a new coronavirus IgG / IgM total antibody. According to the detection method, the IgG / IgM total antibody is detected by using a double-antigen sandwich technology. The detection method specifically comprises the following steps of coating a recombinant new coronavirus RBD protein on a solid-phase carrier, adding a to-be-detected sample into the solid-phase carrier coated with the recombinant new coronavirus RBD protein, incubating and washing, adding biotin-labeled new coronavirus RBD protein into the washed solid-phase carrier, incubating, washing, developing and combining with a standard curve to obtain the antibody content. The technical principle of double antigen sandwich is utilized to detect that the serum stock solution of a healthy person has no false positive, so that the healthy person and a patient can be well distinguished; the method can promote large-scale early screening emergency serological detection of the new coronavirus and related vaccine research and vaccine effect evaluation work.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method and a kit for detecting IgG / IgM total antibodies to the novel coronavirus. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA), also known as enzyme-linked immunosorbent assay, is a qualitative and quantitative detection method that binds soluble antigens or antibodies to polystyrene and other solid-phase carriers, and uses the specific binding of antigens and antibodies to perform immune reactions. Since its inception in the 1970s, ELISA has been widely used in many fields of medical biology as an in vitro diagnostic technology, and can be used to detect antigens, antibodies, and haptens; in practical applications, it can be used for clinical diagnosis and disease monitoring , disease screening, etc. Therefore, it is closely related to immunology, epidemiology and infectious diseases. [0003] At present, there is no enzyme-linked immunoassay k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/543
CPCG01N33/543G01N33/56983G01N33/6854G01N2333/165G01N2469/20
Inventor 王玉聂舒芳
Owner WUHAN AIBO TAIKE BIOTECH CO LTD
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