Preparation method and application of chemiluminescence detection kit for quantitatively detecting TEX101 concentration

A chemiluminescence detection and quantitative detection technology, used in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, biological testing, etc., can solve the problems of subjective factors, poor repeatability, and inability to treat symptoms.

A chemiluminescence detection and quantitative detection technology, used in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, biological testing, etc., can solve the problems of subjective factors, poor repeatability, and inability to treat symptoms.

CN112630451APending Publication Date: 2021-04-09普迪特泰州生物科技有限公司
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  • Preparation method and application of chemiluminescence detection kit for quantitatively detecting TEX101 concentration

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Embodiment Construction

[0014] The present invention is further described and understood by non-limiting examples below.

[0015] The invention provides a method for preparing a chemiluminescence detection kit for quantitatively detecting the concentration of TEX101. The kit includes reagent R1, reagent R2, chemiluminescent substrate solutions A and B.

[0016] Among them, reagent R1 is 0.01M PBS buffer solution containing magnetic beads coated with anti-TEX101 antibody; the pH value of reagent R1 is 7.2, containing 0.1% Tween 20 (v / v), 5% bovine Serum albumin (w / v), 0.5%Proclin300 (v / v); Reagent R2 is the 0.01M PBS buffer solution containing acridinium ester-labeled anti-TEX101 antibody; The pH value of reagent R2 is 7.2, containing 0.1% proclin Temperature 20 (v / v), 5% bovine serum albumin (w / v), 0.5% Proclin300 (v / v).

[0017] Solution A uses hydrogen peroxide solution as the pre-excitation solution, and solution B uses sodium hydroxide solution as the excitation solution.

[0018] The function ...

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Abstract

The invention discloses a preparation method and application of a chemiluminiscence detection kit for quantitatively detecting TEX101 concentration, belongs to the field of in-vitro diagnostic reagents, and provides a TEX101 chemiluminiscence detection kit by researching TEX101 concentration difference results of obstructive azoospermia OA and non-obstructive azoospermia NOA. The kit can rapidly and quantitatively detect the concentration of TEX101 in seminal fluid, the sensitivity of identifying obstructive azoospermia OA and non-obstructive azoospermia NOA is 73%, the specificity is 100%, and the kit has a good prediction value for assisted reproduction results.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a preparation method and application of a chemiluminescent detection kit for quantitatively detecting the concentration of TEX101. Background technique [0002] Azoospermia refers to the complete absence of sperm during ejaculation. It accounts for 10-15% of male infertility cases and 60% of all azoospermia cases. Azoospermia is divided into obstructive azoospermia OA and non-obstructive azoospermia The treatment of azoospermia NOA, obstructive azoospermia OA and non-obstructive azoospermia NOA corresponds to different treatments. [0003] At present, the clinical evaluation of sperm quality mainly adopts: semen analysis and sex hormone serological detection. Semen analysis is the most commonly used detection method, but its analysis results are affected by subjective factors, with low precision and poor repeatability, and its value for male fertility evaluation and c...

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Application Information

Patent Timeline
09 Apr 2021
Publication
CN112630451A
IPC
G01N33/68; G01N33/543; G01N33/532; G01N21/76
CPC
G01N33/689; G01N33/54326; G01N33/532; G01N21/76; G01N2333/47; G01N2800/367
Inventors
奎书毅