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Tomato aquaporin SlTIP1;1for regulating flower stalk shedding

A technology of aquaporin and tomato, which is applied in the field of plant genetic engineering and signal transmission, can solve the problems of destroying undifferentiated state, not regulating tomato aquaporin-related systems, unknown, etc., and contributes to the breeding of high-yield tomato varieties Effect

Pending Publication Date: 2021-04-13
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disruption of the undifferentiated state of cells in the meristem to initiate differentiation, such as expansion and detachment of meristem-like AZ cells, requires molecular mechanisms to turn on water and H 2 o 2 transport; however, the underlying mechanism is unknown, and the tomato aquaporin-associated system that regulates pedicel abscission has not been studied

Method used

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  • Tomato aquaporin SlTIP1;1for regulating flower stalk shedding
  • Tomato aquaporin SlTIP1;1for regulating flower stalk shedding
  • Tomato aquaporin SlTIP1;1for regulating flower stalk shedding

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Embodiment 1

[0035] Embodiment 1: tomato aquaporin SlTIP1; 1 regulates pedicel shedding effect research

[0036] 1.水通道蛋白SlTIP1;1的氨基酸序列为(SEQ ID NO:1) MetProIleAsnGlnIleThrIleGlySerHisGluGluLeuArgHisProGlyAlaLeuLysAlaAlaLeu AlaGluPheIleSerThrLeuIlePheValPheAlaGlyGlnGlySerGlyMetAlaPheAsnLysLeuThr AspGlyValAlaThrProAlaGlyLeuIleSerAlaSerIleAlaHisAlaPheGlyLeuPheValAlaValS erValGlyAlaAsnIleSerGlyGlyHisValAsnProAlaValThrPheGlyAlaPheValGlyGlyAsnIl eThrLeuPheArgGlyIleLeuTyrIleIleAlaGlnLeuLeuGlySerThrAlaAlaCysAlaLeuLeuGl uPheAlaThrGlyGlyMetSerThrGlySerPheAlaLeuSerAlaGlyValSerValTrpAsnAlaPheV alPheGluIleValMetThrPheGlyLeuValTyrThrValTyrAlaThrAlaValAspProLysLysGlyA spLeuGlyValIleAlaProIleAlaIleGlyPheIleValGlyAlaAsnIleLeuAlaGlyGlyAlaPheThr GlyAlaSerMetAsnProAlaValSerPheGlyProSerLeuValSerTrpThrTrpThrHisGlnTrpVal TyrTrpAlaGlyProLeuIleGlyGlyGlyLeuAlaGlyPheIleTyrGluPheIlePheIleSerHisThrHi sGluGlnIleProSerGlyAspPhe

[0037] The overexpression of the aquaporin SlTIP1; 1 has the effect of accelerating the abscis...

Embodiment 2

[0114] The present invention purified a protein of this size from the isolated region, based on SDS-PAGE, and performed mass spectrometric analysis. Mass spectra obtained from the analysis of all fractions were compared to sequences in the Tomato AQP database and peptides were identified according to the intensity-based absolute quantification (iBAQ) method. The mass spectrometry results of spectral counting and statistical analysis showed that a total of eight AQP proteins were identified at 0, 16 and 32 h. Among them, SlTIP1;1 accumulated the highest in the AZ and had an AZ-specific expression pattern during shedding. In the present invention, SlTIP1;1 is knocked out and overexpressed in tomato. Through phenotypic observation and a series of experiments, it is found that knocking out SlTIP1;1 can delay abscission, and at the same time, the abscission rate of overexpressed plants is accelerated compared with wild type; in addition, it is found that Shedding-associated transc...

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Abstract

The invention relates to tomato aquaporin SlTIP1;1 for regulating flower stalk shedding. The amino acid sequence of the aquaporin S1TIP1;1 is shown in SEQ ID NO: 1; overexpression of the aquaporin S1TP1;1 has the effect of accelerating tomato flower stalk shedding, and the aquaporin SlTIP1;1 is not expressed or is not completely expressed to have the effect of delaying the tomato flower stalk shedding; the tomato aquaporin SlTIP1;1 is not expressed through a knockout technology; the aquaporin SlTIP1;1regulates and controls expression through an exfoliation related gene SlERF52, namely overexpressing or inhibiting expression of SlTIP1;1 gene in tomatoes through a genetic transformation means so that tomato flower stalk shedding is adjusted, and the S1TIP1;1 gene is further used for tomato variety breeding.

Description

technical field [0001] The invention relates to the fields of plant genetic engineering and signal transmission, in particular to a tomato aquaporin SlTIP1;1 for regulating flower stalk abscission. Background technique [0002] Plant organ shedding occurs during vegetative and reproductive growth, triggered by developmental signals and environmental stimuli, and occurs in specialized zones known as abscission zones (AZs). Polar transport of the plant hormone auxin from distal organs to the AZ prevents ethylene sensitivity of the abscisal zone With reduced auxin signaling in AZ, AZ cells become sensitized to ethylene, triggering and accelerating abscission. In tomato pedicels, 1-aminocyclopropylacrylic acid-1-carboxylate synthase 1A (ACS1A), ACS2, and ACS6, and ACC oxidase 1 (ACO1) and ACO5 were upregulated during abscission. Ethylene is sensed by its receptors and signals through ethylene response factor 52 (ERF52) to accelerate detachment. Although many studies support t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/02A01H6/82
CPCC07K14/415C12N15/8218C12N15/827
Inventor 李天来王荣李瑞祯许涛
Owner SHENYANG AGRI UNIV
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