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A kind of Heishun piece decoction piece, standard decoction, formula granule ULC-MS characteristic map and its construction method and application

A technology of formula granules and characteristic maps, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as difficult transmission, difficult differences and changes in detection methods, and difficulty in evaluating the process as a whole, achieving good repeatability and accuracy. High and guaranteed clinical efficacy

Active Publication Date: 2022-08-09
JIANGYIN TIANJIANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above reports only conducted HPLC fingerprint research on the decoction pieces of Heishun Tablets, or used RP-HPLC method to establish control indicators. The detection method is difficult to effectively compare and analyze the differences and changes in the characteristic maps of Heishun tablets, standard decoctions, and formula granules. Difficult to overall evaluate and control the technological process from Heishun Pieces decoction pieces to formula granules

Method used

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  • A kind of Heishun piece decoction piece, standard decoction, formula granule ULC-MS characteristic map and its construction method and application
  • A kind of Heishun piece decoction piece, standard decoction, formula granule ULC-MS characteristic map and its construction method and application
  • A kind of Heishun piece decoction piece, standard decoction, formula granule ULC-MS characteristic map and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1: Construction of UPLC-MS characteristic map of Heishun slices, standard decoction, and formula granules.

[0097] 1. Preparation of Solutions

[0098] 1.1 Preparation of the reference medicinal material solution

[0099] Take about 0.5 g of the reference medicinal material of Heishun Tablet, accurately weigh it, put it in a conical flask, add 20 mL of water, weigh it, heat it to reflux for 1 hour, let it cool, weigh it again, make up with water to reduce the weight loss, shake well, filter After that, the filtrate was taken as the control medicinal material solution.

[0100] 1.2 Preparation of reference solution

[0101] Take appropriate amount of neoaconitrile reference substance, benzoyl neoaconitrile reference substance, benzoyl aconitine reference substance, benzoyl aconitine reference substance, and hypoconitine reference substance, respectively, Accurately weigh, add isopropanol-dichloromethane (volume ratio of 1:1) mixed solvent to prepare a stock s...

Embodiment 2

[0290] Example 2: Detection of samples to be tested for Heishan Pieces, standard decoctions, and formula granules.

[0291] The specific method is as follows:

[0292] 1) Preparation of decoction pieces, standard decoctions and / or formula granules of Heishan tablets to be tested;

[0293] 2) carrying out UPLC-MS analysis on the decoction pieces, standard decoction and / or formula granule sample solution prepared in step 1), respectively, to obtain respective UPLC-MS spectrum;

[0294] 3) The UPLC-MS spectrum of the decoction pieces, standard decoction and / or formula granule sample solution to be tested in step 2) corresponds to the UPLC-MS characteristic spectrum of Heishun piece decoction pieces, standard decoction and formula granule provided in Example 1 Compare, evaluate the quality of the samples to be tested for the decoction pieces, standard decoctions and / or formula granules, and compare the differences between the UPLC-MS spectra of the sample solutions of the decocti...

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Abstract

The invention belongs to the technical field of drug analysis, and in particular relates to a UPLC-MS characteristic map of Heishan slices, standard decoction and formula granules, and a construction method and application thereof. In the construction method provided by the present invention, the reference substance and the reference medicinal material are used as the control, and the formic acid aqueous solution-acetonitrile is used as the mobile phase to carry out gradient elution, and the chromatographic conditions and mass spectrometry conditions are rationally controlled, thereby forming Heishan Pieces Decoction and standard decoction , The whole picture of UPLC-MS characteristic spectrum of formula granules improves the quality control of Heishun tablets, and can control the inherent quality of Heishun tablets in the process from decoction pieces to standard decoction to finished formula granules, which is beneficial to The overall evaluation of the scientificity and rationality of the related technological process of Heishun Tablet ensures the stability of the overall quality of Heishun Tablet decoction pieces, standard decoction, and formula granules, so as to ensure the clinical efficacy of Heishun Tablet.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a UPLC-MS characteristic map of Heishan slices, standard decoction and formula granules, a construction method of the characteristic map, and an application of using the characteristic map for sample detection. Background technique [0002] Aconite is the processed product of the sub-root of Aconitum carmichaeli Debx. of the Ranunculaceae plant. Narrow lower, dark brown skin, dark yellow cut surface, hot in nature, pungent, sweet, poisonous, return to the heart, kidney, and spleen meridians, has the effect of returning yang to relieve inversion, tonifying fire and helping yang, dispelling cold and relieving pain. [0003] Studies have shown that the main components of aconite are alkaloids. In the first part of "Chinese Pharmacopoeia" (2015 edition), benzoyl aconitine, benzoyl aconitine and benzoyl aconitine are used as Assay indicators, but not subject to feat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/86
CPCG01N30/02G01N30/72G01N30/8631Y02A50/30
Inventor 翟燕娟张玉张云天吴莉莉李松
Owner JIANGYIN TIANJIANG PHARMA