Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of reagent for detecting HNRNPM expression in preparation of medicine composition for diagnosing and/or prognostically judging liver cancer

A prognostic judgment and composition technology, which is applied in the direction of drug combination, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of unclear biological function and mechanism of action, and achieve anti-cancer effect, inhibition Occurrence and development, effect of good effect

Pending Publication Date: 2021-04-16
ZHONGSHAN HOSPITAL FUDAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have shown that it can promote the proliferation, drug resistance, invasion and metastasis of solid tumors, suggesting that it may be an important tumor-promoting factor. However, the biological function and mechanism of HNRNPM in hepatocellular carcinoma are still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of reagent for detecting HNRNPM expression in preparation of medicine composition for diagnosing and/or prognostically judging liver cancer
  • Application of reagent for detecting HNRNPM expression in preparation of medicine composition for diagnosing and/or prognostically judging liver cancer
  • Application of reagent for detecting HNRNPM expression in preparation of medicine composition for diagnosing and/or prognostically judging liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Material method

[0037] 1. The expression of HNRNPM in HCC and its relationship with clinicopathological features.

[0038] About 240 clinical specimens were collected, and the expression of HNRNPM was detected by immunohistochemical method. According to the corresponding clinical data and prognosis of the specimens, the relationship between HNRNPM expression, clinicopathological features and patient prognosis was analyzed.

[0039] 1.1. Immunohistochemical (IHC) staining

[0040] Liver cancer tissue samples were collected from Zhongshan Hospital Affiliated to Fudan University. Tissues were fixed overnight in 10% neutral formalin, dehydrated, and embedded in low melting point paraffin. Tissue sections with a thickness of 4 μm were cut serially and fixed on siliconized glass slides. Immunohistochemical staining used a streptavidin-peroxidase conjugated method. Each tissue section was deparaffinized, rehydrated, immersed in antigen retrieval solution, boiled at 10...

Embodiment 2

[0054] Knockdown of HNRNPM molecules in liver cancer cell lines can inhibit the proliferation, migration and invasion of liver cancer

[0055] 1. Experimental process

[0056] 1.1. Construction of lentivirus and cell infection

[0057] Cells were infected with a lentiviral vector (U6-sh-HNRNPM-EGFP-IRES-puromycin) purchased from Shanghai Jikai Gene Technology Co., Ltd. to establish a HNRNPM knockdown cell line. HCC cells were infected with recombinant lentiviral transduction units by 0.8 μg / mL polyethylene, and stable cells were selected by 2ug / mL puromycin two weeks later, and the efficiency of RNA interference was evaluated by qRT-PCR and Western blotting.

[0058] 1.2. Cell Migration and Invasion Assays

[0059] Cell migration experiments were performed using Transwell chambers (Becton Dickinson, Franklin Lakes, NJ). will contain 3 x 10 4 200 μL of serum-free DME cell suspension of HCC cells was seeded in the upper chamber of the Transwell, and 400 μL of medium contain...

Embodiment 3

[0073] Knockdown of HNRNPM can inhibit the proliferation and metastasis of orthotopic tumors of mouse liver cancer

[0074] 1. Experimental process

[0075] 1.1. Animal experiments

[0076] All animal experiments were performed in accordance with the "Guide for the Care and Use of Laboratory Animals" (NIH Publication No. 80-23, revised in 1996) and the "Institutional Ethics Guidelines for Animal Experiments" formulated by Fudan University. In the experimental animal room of Zhongshan Hospital affiliated to Fudan University, the animals were kept under pathogen-free conditions. Such as Figure 3A As indicated, two 4-week-old BALB / c female nude mice were subcutaneously injected with 0.1 mL containing 1×10 6 cell suspension of cells. After the subcutaneous tumor grew 4 weeks later, the tumor tissue was cut up, and the cut up liver cancer tissue was planted in the livers of 6 nude mice in each of the two groups, and kept for 4 weeks, and the length (L) and length (L) of each t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a reagent for detecting HNRNPM expression in preparation of a medicine composition for diagnosis and / or prognosis judgment of liver cancer and the medicine composition, whether a patient has the risk of liver cancer or not is judged by detecting the expression level of a molecular marker HNRNPM, and it is shown that high-expression HNRNPM in liver cancer can remarkably predict prognosis of the liver cancer patient. The liver cancer is treated by down-regulating the expression of the HNRNPM by adopting a gene means; the HNRNPM expression molecule is detected before a liver cancer patient is diagnosed, so that the cancer risk is predicted in advance, and high expression of the molecule indicates that the liver cancer risk is high; meanwhile, the postoperative survival of the liver cancer patient can be predicted by detecting the HNRNPM molecular expression before the liver cancer patient is operated, and the higher the HNRNPM molecular expression is detected before the operation, the poorer the prognosis of the postoperative liver cancer patient is; the interference molecule and protein inhibitor combination aiming at HNRNPM can obviously inhibit the generation and development of liver cancer, so that a good anti-cancer effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of a reagent for detecting the expression of HNRNPM in the preparation of diagnosis and / or prognosis judgment of liver cancer and a pharmaceutical composition. Background technique [0002] Worldwide, liver cancer (Hepatocellular carcinoma, HCC) accounts for the second place in all cancer-related deaths, and its morbidity and mortality are increasing year by year. HCC is an aggressive malignant tumor. Due to the various etiologies involved in the occurrence and development of the tumor and the heterogeneity of the tumor, and it is often diagnosed at an advanced stage, the overall prognosis of HCC patients is poor. Although surgery is currently one of the most effective options for the treatment of HCC, about 70% of patients after radical surgery will still relapse within 5 years, so the overall survival rate of HCC is very low. Like other solid tum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
Inventor 代智祝桂琦王亦
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com