Use of Zanthoxylum bungeanum in killing mites and insects, biological control agent and preparation method thereof
A technology of bovine prickly ash and bovine prickly ash essential oil, which is applied in botany equipment and methods, insecticides, acaricides, etc., and can solve the problems that have not yet been reported on the application of bovine prickly ash to kill acarids and insects
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[0047] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method includes: boiling or distilling, condensing and extracting the stem and leaf parts of Zanthoxylum bungeanum.
[0048] In a specific embodiment, the distillation refers to steam distillation for 4-8 hours, 6 hours to fully extract the volatile components in the plant material.
[0049] The poaching refers to boiling with water for 4-8 hours, preferably 6 hours, so that the essential oil components can be boiled out from the fresh plants as completely as possible.
[0050] Preferably, the extraction refers to extraction with n-hexane;
[0051] More specifically, use a 2L separatory funnel, n-hexane:distillate=1:1, keep the n-hexane layer in which the essential oil has been dissolved after layering.
[0052] Preferably, the temperature of the water bath for the extraction is 38-45°C, preferably 4...
experiment example 1
[0057] Experimental example 1, preparation of Zanthoxylum bungeanum essential oil
[0058] The stems and leaves of Zanthoxylum bungeanum were collected, boiled for 6 hours, the obtained oil-water mixture was condensed and recovered, extracted with n-hexane, dried with anhydrous sodium sulfate, and the n-hexane was removed by rotary evaporator, and the obtained ingredient was Zanthoxylum bungeanum essential oil. Wherein, the temperature of the water bath is 40°C.
[0059] The yield of Zanthoxylum bungeanum essential oil is 0.20% (w / w), that is, 0.3g of Zanthoxylum bungeanum essential oil can be extracted from 100g of fresh Zanthoxylum bungeanum plant.
experiment example 2
[0060] Experimental example 2, identification of the components of the essential oil of Zanthoxylum bungeanum
[0061] GC-MS detection method was used to identify the components of Zanthoxylum bungeanum essential oil:
[0062] Detection instruments: gas chromatograph (Agilent6890N) and mass spectrometer (Agilent5973N). .
[0063] Detection conditions: HP-5MS 30mm×0.25mm×0.25μm quartz capillary column, the initial column temperature is 60°C, keep it for 1min, at the rate of 12°C / min, program the temperature to 180°C and keep it for 0.5min, then at the rate of 25°C / min The temperature was programmed to 280°C for 18 minutes, the vaporizer temperature was 280°C, the carrier gas was helium, the column flow rate was 1.0mL / min, and the split ratio was 1:10.
[0064]The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2s.
[0065] 1 μL (100 times acetone dilution) of the plant essential ...
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