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Preparation method of crotonane diterpenoid compound and application of crotonane diterpenoid compound in preparation of nematode killing insecticides

A technology of diterpenoids and crotonane, which is applied in the field of agriculture and can solve problems such as excessive pesticide content

Inactive Publication Date: 2021-04-30
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical control methods that are easy to operate and have quick results are more widely used by farmers, but repeated use of chemical control methods will lead to excessive pesticide content in products and soil. Therefore, people pay more and more attention to ecologically reasonable and effective control of plant parasitic nematodes. methodological research

Method used

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  • Preparation method of crotonane diterpenoid compound and application of crotonane diterpenoid compound in preparation of nematode killing insecticides
  • Preparation method of crotonane diterpenoid compound and application of crotonane diterpenoid compound in preparation of nematode killing insecticides
  • Preparation method of crotonane diterpenoid compound and application of crotonane diterpenoid compound in preparation of nematode killing insecticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Crotonane diterpenoids nematicide activity screening

[0036] Tomato diseased roots infected with root-knot nematodes and soybean diseased roots infected with cyst nematodes were collected. Clean the diseased root with oocysts, pick the oocysts under a dissecting microscope, disinfect the surface with 3% NaClO solution for 3 minutes, and wash with sterile water 3 times. Pick the oocysts into 0.5% NaClO solution, shake for 20 minutes, pass through 200-mesh, 325-mesh and 500-mesh standard sieves once, and rinse the eggs on the 500-mesh sieve with sterile water. Put the egg liquid in an incubator and incubate at 25°C. When a large number of J2 larvae are hatched, collect the nematodes for later use.

[0037] The poisonous effect of compound 1 on nematodes was determined by dipping method. Add 985 μL of fresh M9 buffer to the 24-well plate, and add 10 μL of the nematode suspension at the same time, dissolve the sample to be tested with methanol, take 5 μL of the...

Embodiment 2

[0040] Example 2 Crotonane diterpenoids nematode killing in vivo verification

[0041] When the tomato seedlings are cultivated to 3-4 true leaves, they are transplanted into small flowerpots (12 cm in diameter), with one tomato seedling per pot. One week after transplanting the tomatoes, insert the second instar larvae of the southern root knots, about 1000 per pot. Three days later, the sample and avermectin (positive control, both 10 mg) were formulated into a solution and added to the tomato root, and the corresponding solvent was added to the blank control, and 5 repetitions were set for each treatment. Subsequently, the tomato seedlings are placed in the greenhouse for cultivation, and a certain amount of water is regularly added to prevent water seepage. After 30 days, the tomato roots were dug out and put into clear water to wash off the soil, and the incidence of root-knot nematodes was counted, and the root-knot level was recorded according to the disease symptoms, ...

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Abstract

The invention relates to a preparation method of a crotonane diterpenoid compound and application of the crotonane diterpenoid compound in preparation of nematode killing insecticides, and belongs to the technical field of agriculture. . The crotonane diterpenoid compound is characterized by the following chemical structural formula. According to the invention, the root, stem and leaf parts of euphorbia euphorbia of euphorbia family plant can play a poisoning role on various plant parasitic nematodes in various forms, and the new crotonane diterpenoid compound is separated from the plant. Activity screening proves that the compound has particularly outstanding poisoning activity on plant parasitic nematodes, can be used for preparing nematocides, reduces the number of the plant parasitic nematodes in agricultural production, reduces the harm of the parasitic nematodes and increases the yield of crops.

Description

technical field [0001] The invention relates to a preparation method of crotonane diterpenoids and its application in the preparation of insecticides for killing nematodes, and belongs to the field of agricultural technology. Background technique [0002] Plant parasitic nematodes survive and reproduce by absorbing the nutrients of plants, and also spread other plant pathogens, causing secondary diseases of plants, causing serious damage to agricultural production, and causing economic losses of about 157 billion US dollars every year. At present, the commonly used methods of controlling nematodes in agricultural production mainly include breeding of resistant varieties, biological control, chemical control and so on. Among them, chemical control methods that are easy to operate and have quick results are more widely used by farmers, but repeated use of chemical control methods will lead to excessive pesticide content in products and soil. Therefore, people pay more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/02C07C69/33A01P5/00
CPCA01N37/02C07C69/33C07C2603/40
Inventor 华娟张佳铭骆世洪
Owner SHENYANG AGRI UNIV
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