Hexagrammos otakii sperm productive cryopreservation method

A technology for sperm productivity and six-line fish, applied in the field of sperm freezing, can solve problems such as unrealizable production, and achieve the effects of efficient preservation, good permeability, and reduced damage

Active Publication Date: 2021-05-04
山东省海洋科学研究院青岛国家海洋科学研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the laboratory experiment on the cryopreservation of the sperm of Otaki hexalis has been successful, but it needs to use high-precision instruments and equipment such as an osmometer and a program cooling instrument, which cannot be realized in the production line.

Method used

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  • Hexagrammos otakii sperm productive cryopreservation method
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  • Hexagrammos otakii sperm productive cryopreservation method

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Embodiment 1

[0019] On November 18, 2019, the cryopreservation experiment of Dataki six line fish sperm was carried out in the Otaki six line fish breeding workshop, including the following steps:

[0020] (1) Sperm collection: Fresh sperm is obtained by squeezing sperm from living bodies. Place the mature male fish on dry gauze, cover its head with a towel, rinse the reproductive pores with distilled water until there is no urine or mucus residue, and wipe it off with gauze. Squeeze lightly on the abdomen along the direction from the head to the tail of the fish, use a straw to suck the semen into the cryovial, and store it on ice. Semen whose fresh sperm motility was more than 85% under microscope was selected for cryopreservation test.

[0021] (2) The configuration of the cryopreservation solution: the sterilized natural seawater is the diluent, 85ml; the ethylene glycol is the protective agent, 15ml, and after mixing, a 100ml cryopreservation solution is prepared; the cryopreservation...

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Abstract

The invention belongs to the technical field of sperm freezing, and particularly relates to a Hexagrammos otakii sperm productive cryopreservation method. The method comprises the following steps: after collecting sperms, uniformly mixing seminal fluid with a cryoprotectant, and pre-cooling in a refrigerator at 4 DEG C for 5 minutes; freezing a straw at -20 DEG C for 10 minutes, putting the straw into a straw sleeve, and putting the straw sleeve into an aluminum cryopreservation strip; putting the cryopreservation strip into a pail of a liquid nitrogen tank, putting the pail on the liquid nitrogen surface of the liquid nitrogen tank, cooling for 10 minutes under the condition of -160 DEG C to -180 DEG C, and then putting the cryopreservation strip linto liquid nitrogen for preservation; wherein the cryopreservation liquid is prepared by taking boiled and cooled sterilized natural seawater as a diluent and ethylene glycol with the final concentration of 15% (v / v) as a protective agent. Based on production requirements, the invention provides a simple and batch productive sperm cryopreservation method, and provides technical support for selective breeding of improved varieties of hexagrammos otakii and fishery production; according to the method, the sperm anti-freezing liquid is simple in proportioning, the freezing procedure is relatively convenient and fast, and the requirement for cryopreservation of productive sperms can be met.

Description

Technical field: [0001] The invention belongs to the technical field of sperm freezing, and in particular relates to a method for productive cryopreservation of Otaki hexalis sperm. Background technique: [0002] Hexagrammos otakii, also known as European-style hexagram, is commonly known as yellow croaker, yellow stick, etc. It belongs to the order Scorpaeniformes, the family Hexagrammidae, and the genus Hexagrammos. Otaki hexalis is a cold-temperate bottom fish in the coastal waters. It is distributed in the Yellow Sea, Bohai Sea, Japan, North Korea, and the Far East of Russia. Its meat is delicious, and it is called "Northern Grouper". The fish has strong low temperature tolerance, grows rapidly, and has fresh and tender meat. It can be marketed as a live fish. It is suitable for cage culture in the north and has extremely high economic value. In recent years, with the breakthrough of its artificial breeding technology, the scale of breeding has been continuously expande...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 邹琰宋爱环刘童李翘楚王英俊吴莹莹郭文
Owner 山东省海洋科学研究院青岛国家海洋科学研究中心
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