Hexagrammos otakii sperm productive cryopreservation method

Abstract

The invention belongs to the technical field of sperm freezing, and particularly relates to a Hexagrammos otakii sperm productive cryopreservation method. The method comprises the following steps: after collecting sperms, uniformly mixing seminal fluid with a cryoprotectant, and pre-cooling in a refrigerator at 4 DEG C for 5 minutes; freezing a straw at -20 DEG C for 10 minutes, putting the straw into a straw sleeve, and putting the straw sleeve into an aluminum cryopreservation strip; putting the cryopreservation strip into a pail of a liquid nitrogen tank, putting the pail on the liquid nitrogen surface of the liquid nitrogen tank, cooling for 10 minutes under the condition of -160 DEG C to -180 DEG C, and then putting the cryopreservation strip linto liquid nitrogen for preservation; wherein the cryopreservation liquid is prepared by taking boiled and cooled sterilized natural seawater as a diluent and ethylene glycol with the final concentration of 15% (v / v) as a protective agent. Based on production requirements, the invention provides a simple and batch productive sperm cryopreservation method, and provides technical support for selective breeding of improved varieties of hexagrammos otakii and fishery production; according to the method, the sperm anti-freezing liquid is simple in proportioning, the freezing procedure is relatively convenient and fast, and the requirement for cryopreservation of productive sperms can be met.

Description

Technical field: [0001] The invention belongs to the technical field of sperm freezing, and in particular relates to a method for productive cryopreservation of Otaki hexalis sperm. Background technique: [0002] Hexagrammos otakii, also known as European-style hexagram, is commonly known as yellow croaker, yellow stick, etc. It belongs to the order Scorpaeniformes, the family Hexagrammidae, and the genus Hexagrammos. Otaki hexalis is a cold-temperate bottom fish in the coastal waters. It is distributed in the Yellow Sea, Bohai Sea, Japan, North Korea, and the Far East of Russia. Its meat is delicious, and it is called "Northern Grouper". The fish has strong low temperature tolerance, grows rapidly, and has fresh and tender meat. It can be marketed as a live fish. It is suitable for cage culture in the north and has extremely high economic value. In recent years, with the breakthrough of its artificial breeding technology, the scale of breeding has been continuously expande...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the laboratory experiment on the cryopreservation of the sperm of Otaki hexalis has been successful, but it needs to use high-precision instruments and equipment such as an osmometer and a program cooling instrument, which cannot be realized in the production line.

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