Production method of novel organic selenium-enriched bean sprouts
A production method and organic technology are applied in the production field of new organic selenium-enriched bean sprouts, and can solve the problems of many links of selenium-enriched effect, long process flow, complicated operation and the like
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[0034] (1) Preparation of polypeptides: extract proteins from animals and plants by alkali-dissolving and acid-precipitating methods, or directly crush and refine animals and plants, then add protease to the obtained proteins or slurry for enzymatic hydrolysis, inactivate enzymes, and make polypeptide solutions The specific operation is: first ultrafinely pulverize the animals and plants, then mix the pulverized animals and plants with water at a mass volume ratio of 1:15-40, adjust the pH to 8.5-10.5 with 0.5-1.5 wt% alkali solution, and ultrasonically After extraction for 20-45min, extract in a water bath at 60-90°C for 45-150min, filter, adjust the pH of the filtrate to 2.5-4.5 with hydrochloric acid for protein precipitation, centrifuge at 4000-6000r / min for 5-10min to obtain the precipitated protein, wash with water until neutral and then again centrifuge, and freeze-dry the obtained precipitate to obtain the protein; the alkaline solution is NaOH solution or KOH solution....
Embodiment 1
[0043] (1) Disinfection: After the soybeans are removed from impurities and unfulfilled particles, immerse them in a 0.5% sodium hypochlorite solution for disinfection for 30 minutes, and then rinse with clear water;
[0044] (2) Preparation of selenium-enriched nutrient solution: dissolving the selenium-containing polypeptide solution with a content of 0.5% in water, and making an organic selenium solution with a selenium content of 1 mg / L in proportion for subsequent use;
[0045] (3) Soaking: Soak the sterilized soybeans in the selenium-enriched nutrient solution in (2), the ratio of the volume of the selenium-enriched nutrient solution to the mass of soybeans is 2.5:1, the temperature is 15° C., and the soaking time is 6 hours;
[0046] (4) Germination: Put the soybeans in a germination box and cultivate them under constant temperature and dark conditions at 30°C. The germination time is 48 hours, and spray water every 4 hours during this period to keep them moist;
[0047...
Embodiment example 2
[0049] (1) Disinfection: After the soybeans are removed from impurities and unfulfilled particles, immerse them in a 1.0% sodium hypochlorite solution for disinfection for 5 minutes, and then rinse with clear water;
[0050] (2) Preparation of selenium-enriched nutrient solution: dissolving the selenium-containing polypeptide solution with a content of 1% in water, and making an organic selenium solution with a selenium content of 5 mg / L in proportion for subsequent use;
[0051] (3) Soaking: Soak the sterilized soybeans in the selenium-enriched nutrient solution in (2), the ratio of the volume of the selenium-enriched nutrient solution to the soybean mass is 5:1, the temperature is 30° C., and the soaking time is 2 hours;
[0052] (4) Germination: Put the soybeans in a germination box and cultivate them under constant temperature and dark conditions at 15°C. The germination time is 96 hours, and spray water every 12 hours during this period to keep them moist;
[0053] (5) Cl...
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