Micro-fluidic chip nematode adsorbing and fixing device and use method thereof

Abstract

The invention discloses a micro-fluidic chip nematode adsorbing and fixing device and a use method thereof. By using the micro-fluidic chip side adsorption device, nematodes can be well adsorbed and fixed. The fixing effect is equivalent to that of conventional glue adhesion and fixation, the effect is good, the fixing time is longer, the activity of nematodes can be well kept, and even if the fixing time is as long as two hours, the device is suitable for long-term experiment operation. Nematodes are adsorbed and fixed instead of being fixed by pressure, so that the influence on the pressure in the nematodes is small, and the method is a physical fixing method. According to the nematode fixing operation steps, the nematodes can be fixed in a normal physiological posture, the influence of the fixing operation on the physiological behaviors of the nematodes is reduced to the minimum as much as possible, after the nematodes are released after being fixed, the activity of the nematodes is recovered quickly, and the nematodes cannot be recovered through a glue adhesion method. The device is controllable in nematode sampling speed and convenient to operate. And head stimulation and neuron imaging analysis of chemical medicines can be synchronously implemented, so that the experiment efficiency can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips and nematode research methods, and in particular relates to a device for absorbing and fixing nematodes by microfluidic chips and a method for using the same. Background technique [0002] As a model organism, Caenorhabditis elegans has been widely used in life science research and has made major breakthroughs. Nematodes have the advantages of simple structure, short life cycle, easy cultivation and storage, clear genetic background and homology with human genes. The nematodes are composed of 959 cells and are relatively small. The adult worms are only 1 mm long, and the larvae are even smaller and need to be observed with a microscope. The size of the microfluidic channel of the microfluidic chip is on the order of microns, which is well matched with the size of the nematode, so the microfluidic chip can be used to operate the nematode. [0003] When performing microscopic imaging an...

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the deficiencies in the prior art, the present invention provides a microfluidic chip adsorption and immobilization nematode device and its use method. The channel height and the reduction of the number of side suction channels solve the problem that nematodes will be sucked into the side suction channels

Images