Bioactive peptide with amino acid structure VVRKPLNKEGKKP as well as preparation method and application of bioactive peptide

A bioactive peptide and amino acid technology, applied in the field of protein

Active Publication Date: 2021-05-18
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the function of the polypeptide fragment of 40S ribosomal protein S6 protein in the prior art

Method used

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  • Bioactive peptide with amino acid structure VVRKPLNKEGKKP as well as preparation method and application of bioactive peptide
  • Bioactive peptide with amino acid structure VVRKPLNKEGKKP as well as preparation method and application of bioactive peptide
  • Bioactive peptide with amino acid structure VVRKPLNKEGKKP as well as preparation method and application of bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The artificial synthesis of embodiment 1 active peptide VVRKPLNKEGKKP and VVRKPLNKEGKKPR

[0035] 1. Synthesis of Bioactive Peptides

[0036] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0037] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0038] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0039] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful...

Embodiment 2

[0078] Example 2 Immunomodulatory Activity Experiment of Bioactive Peptides

[0079] 1. Determination of the amount of bioactive peptide VVRKPLNKEGKKP that promotes nitric oxide induction in macrophages (Griess method)

[0080] 1. Experimental reagents and instruments:

[0081]Reagents: experimental animal balb / c mice (male 6-8 weeks old) spleen lymphocyte-derived bioactive peptide VVRKPLNKEGKKP; LPS, purchased from Sigma Company; neutral red staining solution, produced by Beyond Biotechnology Research Institute.

[0082] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus; Dragon WellscanMK3 microplate reader Labsystems.

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide with an amino acid structure VVRKPLNKEGKKP as well as a preparation method and application of the bioactive peptide. The amino acid sequence of the bioactive peptide VVRKPLNKEGKKP is shown as SEQ ID NO: 1, and the amino acid sequence of the bioactive peptide VVRKPLNKEGKKPR is shown as SEQ ID NO: 2. An in-vitro immune regulation function experiment proves that the bioactive peptides VVRKPLNKEGKKP and VVRKPLNKEGKKPR have a very good immune regulation function. The VVRKPLNKEGKKP and the VVRKPLNKEGKKPR disclosed by the invention have the capability of promoting the increase of nitric oxide induction amount of macrophages under a certain concentration condition, so that the capability of resisting external pathogen infection of a body is improved, the morbidity of the body is reduced, meanwhile, the proliferation of in-vitro macrophages is promoted, the immune function of the body is exerted, and the bioactive peptide has very important significance for developing food, health care products and medicines with immune regulation functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide with an amino acid structure VVRKPLNKEGKKP and a preparation method and application thereof. Background technique [0002] Because of its many potential biological functions, bioactive peptides have attracted more and more attention and become one of the hotspots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3,000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. However, due to the large number of living peptides, there are still many peptides to be studied for their related properties. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies ...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P29/00A61P37/02A61K8/64A61Q19/00A23L33/18
CPCC07K14/47A61P29/00A61P37/02A61K8/64A61Q19/00A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55A23V2200/30
Inventor 张少辉郭婷婷占文静张伯宇栾媛媛李政
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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