Preparation method of ultrasonic treatment fat extract, extract and application

Abstract

The invention belongs to the technical field of biological materials, and discloses a preparation method of an ultrasonic treatment fat extract, the extract and application. The preparation method comprises the following steps of: centrifuging fat tissues and taking an intermediate fat cell layer; performing ultrasonication on the obtained intermediate fat cell layer; and centrifuging the broken cells to obtain a transparent liquid layer, namely the ultrasonic treatment fat extract. The ultrasonic treatment fat extract disclosed by the invention can be applied to preparation of drugs for promoting angiogenesis and / or neogenesis, preparation of drugs for improving insufficient or low angiogenesis and preparation of drugs for adjusting angiogenesis and state of organisms or tissues. According to the preparation method disclosed by the invention, after the first step of centrifugation is completed, the cells are directly subjected to ultrasonication and secondary centrifugation, the fat extract from the ultrasonically broken cells is obtained in one step, and the whole process only needs less than 30 minutes. According to the preparation method disclosed by the invention, mechanical emulsification and repeated freezing and thawing are not needed, the acquisition time is shorter, the contained cell factors are richer, and the effect is better.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a preparation method, extract and application of ultrasonically treated fat extract. Background technique [0002] At present, nano-fat is the product obtained by mechanically cutting adipose tissue and then chylolysis, which contains lipid droplets, stromal cells and various growth factors, and is used in soft tissue filling. Previous studies have found that subcutaneous injection of nano-fat into photoaged nude mice can promote dermal regeneration and neovascularization, and nano-fat assisted particle fat transplantation can improve the survival rate of fat by promoting angiogenesis. Because nanofat contains stromal cells and growth factors, previous studies have believed that cellular components in nanofat play an important role, but it is still unclear whether cellular components are necessary in the study of assisted particle fat transplantation. ...

AI Technical Summary

Problems solved by technology

[0004] However, the existing extraction method needs to go through crushing, first centrifugation, emulsification and second centrifugation to collect the transparent liquid in the middle layer. During this period, it needs to go through emulsification and repeated freezing and thawing to break the cells. The operation process is more and the time consumption is relatively long. long

[0005] Through the above analysis, the existing problems and defects of the prior art are: the existing extraction method of fat extract has many operating procedures, the extraction steps are cumbersome, the time consumption is long, there are too many manual steps, and the extracted product is not good.

[0006] The difficulty in solving the above problems and defects is: emulsification and freeze-thaw both require manual multi-step operations in the operation process, which cannot be simplified into standardized instruments to obtain; in terms of time, it takes a long time to break cells through emulsification and freeze-thaw The operation, especially the freeze-thaw step, takes 12 hours or even a day to reach the required temperature; if the operation process and time do not meet the requirements, the quality of the extracted product is poor

[0007] The significance of solving the above problems and defects is: to solve the problems of more operation procedures, too many manual steps, cumbersome extraction steps, and long consumption of fat extracts, etc., will make it easier to obtain fat extracts and facilitate the formulation of strict industry standards , at the same time, the fat extract can be obtained immediately after the operation, without waiting for the patient to come to the hospital for the second time after going home

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