Obvious micro -imaging system based on dual -core optical fiber light control and dynamic scattered spots

Abstract

The invention provides a microscopic imaging system based on double-core optical fiber optical manipulation and dynamic speckle illumination. The utility model is characterized in that: the light manipulation part of the device is composed of two two-core optical fibers whose output end faces are processed into a specific angle and installed opposite to each other. The laser beams are respectively coupled into a two-core fiber through a single-mode fiber to form a focused light field near the output end face to stably capture the cells to be tested. By adjusting the output power of each core of the other two-core fiber, the cell is rotated about a specific axis. After the cells rotate to a certain angle and reach a stable state, the tomographic images of the cells are obtained by using dynamic speckle illumination widefield fluorescence microscopy, and finally the three-dimensional structural images of the cells are reconstructed. The system constructed by the invention can realize the acquisition of three-dimensional structural images of living single cells with high temporal and spatial resolution, has the characteristics of simple structure, low cost, simple operation, etc., and has a wide range of research fields such as biology, medicine and life sciences. application prospects.

Description

(1) Technical field [0001] The invention relates to a microscopic imaging system based on double-core optical fiber optical manipulation and dynamic speckle illumination, which can be used for imaging with high temporal resolution and high spatial resolution of the three-dimensional structure of living single cells, and belongs to the field of biophotonics. (2) Background technology [0002] Cells are the basic unit of life structure and function, and the in-depth study of cells is the key to revealing the mysteries of life phenomena, transforming life and overcoming diseases. Analytical studies of single cells enable the investigation of phenomena and mechanisms not observed in large populations of cells. On the one hand, there are different levels of living systems. Biological sciences want to study different levels of living systems and their interrelationships, and the first thing to study is cells. On the other hand, most of the reactions in organisms are carried out i...

AI Technical Summary

Problems solved by technology

The traditional optical tweezers system needs to use a large numerical aperture objective lens to form a strong optical potential well at the focal point to achieve cell capture and manipulation. The system is bulky, has poor flexibility, is expensive, and is subject to many factors during use, such as and substrate compatibility limitations

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