Vaccine containing codon-optimized mycobacterium tuberculosis fusion protein AH

A Mycobacterium tuberculosis, codon optimization technology, applied in the field of molecular biology, can solve problems such as poor protection effect
CN112852848AActive Publication Date: 2021-05-28CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-05-28

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Abstract

The invention provides a codon-optimized mycobacterium tuberculosis fusion gene, a corresponding fusion protein, a vaccine, application in tuberculosis prevention and a preparation method. The method comprises the following steps: carrying out fusion expression on a mycobacterium tuberculosis infection early secretion antigen Ag85A and a latency expression antigen HspX, connecting the two antigen genes by using flexible Linker (GGGGS)3, carrying out codon optimization on a fusion gene sequence according to the DNA codon bias of escherichia coli, and then connecting the gene sequence to a vector pET-30a(+) for escherichia coli expression. The purified recombinant protein Ag85A-HspX (AH for short) and polyinosinic cell (PolyI:C) are used for intranasal immunization of mice, and the result proves that the recombinant protein has good immunogenicity and a protective effect of resisting mycobacterium bovis infection.
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Description

Technical field

[0001] The present invention belongs to the field of molecular biology and vaccine, which provides a codon optimized tuberculosis fusion gene, and corresponding fusion protein, vaccine, in preventing tuberculosis applications and preparation methods.Background technique

[0002] Tuberculosis is a chronic infectious disease mainly caused by Mycobacterium Tuberculosis, referred to as M.TB and Nao M. M. Bovis, still threatening global health. As one of the world's ten causes, tuberculosis is also a single killer in a single infectious disease. And the drug tuberculosis is still a global public health threat. Therefore, there is an urgent need to develop safe and effective tuberculosis vaccines to alleviate global tuberculosis burden. Because the Bacille Calmette-Guérin, BCG has more limitations, such as invalid for adult tuberculosis and latent infection, more and more new tuberculosis vaccines are continuous development and clinical verification. According to statistics, 14 ...

Claims

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