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Preparation method of spinal cord regeneration and repair material

A technology for repairing material and spinal cord, applied in the field of preparation of spinal cord regeneration repair material, can solve the problems of unable to form precise three-dimensional structure, unable to adapt to the shape of spinal cord injury, unfavorable for cell reproduction and growth, etc., to achieve the effect of promoting reproduction

Inactive Publication Date: 2021-06-04
WEIFANG AOJING MEDICAL RES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantages of existing spinal cord regeneration and repair materials are: 1. It cannot form an accurate three-dimensional structure and cannot adapt to the shape of the spinal cord injury
2. The micropores formed by the engineering scaffold are small, which is not conducive to the reproduction and growth of cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1) Make a mold according to the shape of the bone marrow, and the mold is a cylinder that penetrates up and down. The step 1) includes the following specific steps: step 1.1) designing a three-dimensional model of the spinal cord with computer-aided design software; step 1.2) making a mold according to the three-dimensional model of the spinal cord.

[0020] Step 2) A plurality of filaments running through both ends are placed in the mold, and the filaments are preferably stainless steel wires.

[0021] Step 3) Close the lower end of the mold.

[0022] Step 4) Inject the bio-slurry into the mold, put it into a lyophilizer, remove the solvent in the bio-slurry by heat-induced phase separation, and obtain a solid with a large number of micropores; the preparation method of the bio-slurry includes the following steps: step 4.1) Dissolve type I collagen and chitosan with a mass ratio of 2:1 in 0.05 mol / L acetic acid solution, and stir at 800 rpm for 60 minutes in a co...

Embodiment 2

[0028] Step 1) Make a mold according to the shape of the bone marrow, and the mold is a cylinder that penetrates up and down. The step 1) includes the following specific steps: step 1.1) designing a three-dimensional model of the spinal cord with computer-aided design software; step 1.2) making a mold according to the three-dimensional model of the spinal cord.

[0029] Step 2) A plurality of filaments running through both ends are placed in the mold, and the filaments are preferably stainless steel wires.

[0030] Step 3) Close the lower end of the mold.

[0031] Step 4) Inject the bio-slurry into the mold, put it into a lyophilizer, remove the solvent in the bio-slurry by heat-induced phase separation, and obtain a solid with a large number of micropores; the preparation method of the bio-slurry includes the following steps: step 4.1) Dissolve type III collagen and chitosan with a mass ratio of 3:2 in 0.05 mol / L acetic acid solution, and stir at 800 rpm for 60 minutes in a ...

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PUM

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Abstract

The invention discloses a preparation method of a spinal cord regeneration and repair material. The preparation method comprises following steps: step 1) a mold is prepared according to the shape of bone marrow, and the mold is a vertically through cylinder; step (2) a plurality of thin filaments penetrating through the two ends are placed in the mold; step (3) the lower end of the mold is closed; step (4) biological pulp is injected into the mold, the mold is put into a freeze dryer, and a solvent in the biological pulp is removed through thermally induced phase separation, so that a solid with a large number of micropores is obtained; and step 5) the solid is taken out of the cylinder and the thin filaments are extracted out to obtain a solid stent with a large number of micropores and a plurality of axial channels. A spinal cord three-dimensional model prepared through three-dimensional modeling can be better matched with the shape of a spinal cord injury part; and the axial channels are consistent with the nerve conduction direction, seed cell proliferation and differentiation can be guided, new synaptic contact is established, the micropores are used for remaining cell growth factors, and seed cell reproduction and growth in the axial channels are promoted.

Description

technical field [0001] The invention relates to a preparation method of a spinal cord regeneration and repair material. Background technique [0002] Spinal cord injury is a common clinical disease. Spinal cord injury is caused by a variety of reasons, such as tumor, inflammation, trauma, etc. can lead to spinal cord injury. Spinal cord injury has a great impact on patients. The most common is the loss of nerve function below the spinal cord injury surface, and more serious ones can also cause functional defects in the cardiovascular system, respiratory system, and digestive system. These functional defects will affect the normal life of patients. [0003] Spinal cord injury is not only destructive, but also difficult to recover, because it is difficult to repair nerve conduction pathways through self-neurons forming synapses at the complete transection after spinal cord injury. So until now, spinal cord regeneration is still an important topic in clinical medicine. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/26A61L27/54A61L27/56
CPCA61L27/26A61L27/56A61L27/227A61L27/54A61L2300/414C08L89/00C08L5/08
Inventor 宋天喜李洪景刘洋崔孟龙仇志烨朱艳泽胡艳丽何志敏崔云李良才朱金亮
Owner WEIFANG AOJING MEDICAL RES CO LTD