Plant source conduit and application thereof to nerve injury repair
A technology of plant source and catheter, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of high product cost and complicated process, and achieve the effects of convenient material collection, promotion of cell adhesion, and good biodescriptiveness
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Embodiment 1
[0100] A method for preparing plant-derived conduits, comprising the steps of:
[0101] (1) Cut a 1cm-long catheter from the internode section of the stem of A. japonicus, and soak it in deionized water for 24 hours;
[0102] (2) Place the catheter obtained in step (1) in a round bottom flask with 300 mL of 10% sulfuric acid solution in a water bath at 60°C for 40 minutes, then wash it with double distilled water to neutrality; place the catheter in a 300 mL mass fraction In a sodium hydroxide solution with a fraction of 10%, react in a water bath at 60°C for 20 minutes, take it out, and wash it with double distilled water until neutral;
[0103] (3) Treat the catheter obtained in step (2) with trypsin at 37°C for 30min, then with papain at 37°C for 305min; wherein the amount of trypsin is 0.5% of the catheter quality, and the amount of papain is 1% of the mass of the conduit;
[0104] (4) The catheter obtained in step (3) is carefully removed with a silver probe from the fl...
Embodiment 2
[0107] Inoculation of EMSCs on the surface of plant-derived catheters to repair nerve damage includes the following steps:
[0108] (1) Protein modification of plant-derived vessels:
[0109] Soak the plant source catheter prepared in Example 1 with fibrinogen solution (40mg / ml) for 10min, and take out the catheter; drip thrombin solution (40U / ml) while rotating the catheter on the surface of the catheter, so that the fibrinogen solution is The surface of the catheter was solidified into a gel-like coating; the catheter was taken out and dried in a vacuum oven at 37°C for 1.5 hours to obtain a protein-modified catheter, which was stored in a refrigerator at 4°C for later use;
[0110] (2) The cultivation of EMSCs;
[0111] SD rats were sacrificed by cervical dislocation. Under aseptic conditions, the skin and nasal bones were cut through the nostrils along the nasal cavity to the inner canthus in a sterile operating table, and the nasal septum was completely removed. ℃ in PB...
Embodiment 3
[0131] Inoculating SHH-EMSCs on the surface of plant-derived catheters to repair nerve damage includes the following steps:
[0132] (1) Protein modification of plant-derived vessels:
[0133] Soak plant-derived catheters in laminin solution (1 μg / ml) for 1 h, dry in a vacuum oven at 37 °C, then soak in genipin solution (concentration: 1%) for 1 h, place Dry in a vacuum oven at 37°C. This process was repeated three times to obtain plant-derived conduits, which were stored in a 4°C refrigerator for later use;
[0134] (2) Construct EMSCs transfected with SHH gene;
[0135] 1. The cultivation of EMSCs: the same as the step (2) of Example 2
[0136] SD rats were sacrificed by cervical dislocation, the nose and surrounding area were disinfected with iodine, and EMSCs were extracted in a sterile operating table. Specific operation: use scissors to remove the cartilage and soft tissue of the nasal head, extend into the nasal cavities on both sides and cut open, lift the rat's ja...
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