Cell culture device

A cell culture and incubator technology, which is applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as pollution, internal pollution, and reduced success rate of culture, and achieve improved Survival rate, effect of pollution prevention

Inactive Publication Date: 2021-06-04
绍兴市高砚智生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a cell culture device, which solves the problem that in the prior art, most of the cell culture devices are open-type culture, and the experimenter needs to open the device for observation, which causes great pollution to the inside , the success

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Experimental program
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Embodiment 1

[0042] see Figure 1-2 , the present invention provides a technical solution: a cell culture device, including a cell culture device body 1 composed of an incubator 2, an oxygen storage tank 3, an observation mechanism 4 and a debugging mechanism 5, and a culture chamber 21 is provided inside the incubator 2 , the bottom of the inner wall of the culture cavity 21 is fixedly connected with a heating mechanism 22, and the top of the heating mechanism 22 is connected with a water storage tank 23, and the top of the water storage tank 23 is successively provided with an air jet plate 24 and a cultivation frame 25, and the outside of the air jet plate 24 is connected with the cultivation The inner wall of the cavity 21 is fixedly connected, and the inside of the cultivation cavity 21 is provided with a rotating rod 26, and the outside of the rotating rod 26 is fixedly connected with the inside of the cultivation frame 25, and the top of the air jet plate 24 is provided with an air i...

Embodiment 2

[0046] see Figure 1-5 On the basis of Embodiment 1, the present invention provides a technical solution: the culture frame 25 includes a culture plate 251, the inside of the culture plate 251 is slidably connected with a culture support plate 252, and the inside of the culture support plate 252 is slidably connected with the culture dish 28 The outer side of the culture support plate 252 is fixedly connected with a magnetic bar 253 , the outer side of the culture tray 251 is fixedly connected with the inner wall of the culture cavity 21 , and the inside of the culture tray 251 is fixedly connected with the rotating rod 26 .

[0047] The observation mechanism 4 includes an observation frame 41. The outside of the observation frame 41 is fixedly connected to the inside of the incubator 2. The bottom of the observation frame 41 runs through the incubator 2 and extends to the inside of the incubator 2. The inside of the observation frame 41 is sequentially arranged from top to bot...

Embodiment 3

[0052] see Figure 1-8 , On the basis of Embodiment 1 and Embodiment 2, the present invention provides a technical solution: the air injection mechanism 27 includes an air injection block 271, an air delivery pipe 272 is arranged inside the air injection block 271, and an air injection valve is fixedly connected to the top of the air injection block 271 273, the top of the air delivery pipe 272 communicates with the air injection valve 273, the bottom of the air delivery pipe 272 communicates with an air delivery ring block 274, and the bottom of the air injection block 271 is provided with a slow flow groove 275.

[0053] The gas transmission ring block 274 includes a receiving bar 2741, the outer side of the receiving bar 2741 is fixedly connected with the inside of the air injection block 271, and the inner part of the receiving bar 2741 is provided with a receiving port 2742, and the receiving port 2742 communicates with the bottom of the gas delivery pipe 272, and the rece...

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Abstract

The invention discloses a cell culture device. The cell culture device comprises a cell culture device body composed of a culture box, an oxygen storage tank, an observation mechanism and a debugging mechanism, wherein a culture cavity is formed in the culture box, a heating mechanism is fixedly connected to the bottom of the inner wall of the culture cavity, the top of the heating mechanism communicates with a water storage tank, an air injection plate and a culture frame are sequentially arranged at the top of the water storage tank, and the outer side of the air injection plate is fixedly connected with the inner wall of the culture cavity. The invention relates to the field of biotechnology. The cell culture device solves the problems that in the prior art, most of cell culture devices are in open culture, experimenters need to open the devices for observation, the interior is seriously polluted, the culture success rate is greatly reduced, each part cannot be specifically observed due to direct observation of a very small part, the observation record is inaccurate, meanwhile, the temperature of a culture dish cannot be well constant, and bacterial contamination is also easily caused by internal heating.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a cell culture device. Background technique [0002] Cell culture refers to a method of simulating the internal environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. No matter for the whole bioengineering technology or one of the biological cloning technologies, cell culture is an indispensable process. Cell culture itself is a large-scale cloning of cells. , Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used tech...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12M1/12C12M1/10C12M1/04C12M1/02
CPCC12M23/24C12M23/48C12M23/52C12M29/06C12M29/20C12M29/26C12M37/00C12M37/04C12M41/20C12M41/34C12M41/46
Inventor 费小涛
Owner 绍兴市高砚智生物科技有限公司
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