74 results about "Bacteriological contamination" patented technology

A microfluidic device for culturing cells, termed a microscale cell culture analog (μCCA), is provided. The microfluidic device allows multiple cell or tissue types to be cultured in a physiologically relevant environment, facilitates high-throughput operation and can be used for drug discovery. The microfluidic device uses gravity-induced fluidic flow, eliminating the need for a pump and preventing formation of air bubbles. Reciprocating motion between a pair of connected reservoirs is used to effect the gravity-induced flow in microfluidic channels. Bacterial contamination is reduced and high throughput enabled by eliminating a pump. The microfluidic device integrates a pharmacokinetic-pharmacodynamic (PK-PD) model to enable PK-PD analyses on-chip. This combined in vitro / in silico system enables prediction of drug toxicity in a more realistic manner than conventional in vitro systems.

The invention relates to the field of high polymer materials, and provides an antibacterial master batch, which comprises the following raw materials in parts by weight: 70-90 parts of polypropylene resin, 3-6 parts of nano-silver loaded graphene, 1-3 parts of a dispersing agent and 0.4-0.8 part of a lubricating agent, wherein the nano-silver loaded graphene has the advantages of both graphene andnano-silver. The antibacterial master batch obtained after melt granulation with polypropylene resin has excellent antibacterial property, solves the problems that a product produced from a polypropylene material in the prior art does not have antibacterial property and is easily polluted by bacteria, and can be applied to production of disposable tableware.

Method for the preparation of at least one compound with biological properties from blood, where said method is performed in sealed tubes at a pressure below atmospheric pressure, thereby reducing or preventing the bacterial contamination of the compound through handling. The method comprises the repetition, for as many times as is required, of the following steps: connecting a second container that is vacuum-sealed to an extraction device connected in turn to a first container that contains blood separated into fractions, waiting for a period of time until the required fraction(s) is / are transferred, and removing said second container, with it thus being possible to obtain several second containers with different compounds for different medical applications including biological therapies. The steps may be performed in a closed system, without removing the caps of the containers, or alternatively the first container may be opened prior to the introduction of the extraction device.

The invention belongs to the field of edible fungi culture medium processing devices and particularly discloses a stirring device for culture materials of edible fungi. The stirring device comprises a rack, a stirring kettle and a heating tank, wherein the stirring kettle comprises a kettle body, a stirring shaft, a stirring blade and a stirring motor; the kettle body is obliquely fixed on the rack, is cylindrical and comprises a side wall, a sealing ring, an inner wall and an outer wall; a hollow interlayer is arranged between the inner wall and the outer wall which are connected via the sealing ring; a water outlet pipe is arranged on the sealing ring at the relatively low end of the kettle body; steam outlets are uniformly formed in the inner wall; a steam inlet pipe is arranged on the sealing ring at the relatively high end of the kettle body; the stirring blade is spirally wound and fixed on the stirring shaft; the stirring shaft is hinged to the side wall; a belt plate connected with the stirring motor via a belt is arranged on the stirring shaft. The stirring device provided by the invention can not only uniformly stir a culture medium but also effectively protect the culture medium from mould and bacterial contamination.

The invention relates to the technical field of biological pharmacy, in particular to a cell culture consumable packaging method meeting the requirements of GMP (good manufacturing practices). According to the technical scheme, the cell culture consumable packaging method comprises the steps that first, cell culture consumables are produced according to a normal non-sterilization process; second,three-layer vacuum packaging is performed on products based on the produced non-sterilized cell culture consumables; and third, irradiation sterilization is performed on the vacuum-packaged products to obtain final products. According to the cell culture consumable packaging method meeting the requirements of GMP, the integrity of the product packaging can be intuitively judged through the three-layer vacuum packaging, damage is not prone to being caused, pollution to clean areas is avoided, the products can be better ensured to be sterilized, the risk of bacterial contamination is reduced, and the cell culture consumable packaging method is suitable for high-level purification requirements.

The invention provides a composite fresh-keeping method of coregonus peled. The composite fresh-keeping method comprises the following technology steps of (1) pretreating coregonus peled; (2) immersing the pretreated coregonus peled in a composite biological fresh-keeping agent; (3) performing vacuum packing; and (4) performing fresh-keeping storage with slurry ice. According to the composite fresh-keeping method, compounding sterilization and oxidation-resistant effects of the composite biological fresh-keeping agent are mainly used, so that growth and propagation of microorganisms in the coregonus peled are effectively restrained, and oxidizing action of fish meat lipid is alleviated; vacuum packing is adopted, so that good effects of protecting color, keeping fresh, guaranteeing quality, preventing bacteriological contamination and the like are achieved; and the fresh-keeping storage with the slurry ice is adopted, so that the fresh-keeping period of the coregonus peled is greatly prolonged, and the composite fresh-keeping method has favorable fresh-keeping effects. According to the method, the fresh-keeping technology and the low-temperature fresh-keeping technology of the biological fresh-keeping agent are performed simultaneously, so that synergistic reaction is completely exerted, and the best effect of fresh keeping can be realized based on that the quality and the freshness of the coregonus peled are guaranteed.

The present invention relates to the field of biotechnology, more specifically the field of food biotechnology: the control of bacterial contamination in ready-to-eat foods by a bacteriophage composition.

The invention discloses a selective culture medium for separating a Fusarium graminearum species complex from the air. According to the culture medium, toxoflavin, hymexazol, aliette, quintozene and Rifampicin are added to a PDA culture medium. It is proved through experiments that when the selective culture medium is applied to separation of Fusarium graminearum species complex in the air, deep-wine characteristic colonies of Fusarium graminearum appear, and the problems can be solved that Fusarium graminearum cannot be distinguished easily from other Fusarium, and serious contamination is caused by multiple undesired fungi and bacteria during separation; and the selective culture medium has the characteristics of feasibility, high accuracy, practicality, quickness and simple preparation.

A gel formulation is combined with a bactericidal concentrate which is a liquid mixture of about 1000 parts by volume of ultra pure water having an electrical resistance of 16-26 megohms, total dissolved solids of less than 0.04 parts per million and a specific conductance of less than 0.10 mho with about 1.2 to 3 parts by volume of Willard Water as prepared in accordance with U.S. Pat. No. 3,893,943. The bactericidal liquid concentrate in the amount of 3.5 to 12 ml in one liter of gel formulation is applied directly to a skin surface area having a burn or other dermal lesion to protect the area from bacterial contamination.

A foot actuated toilet seat or seat / lid lifting and self-lowering mechanism (100) designed specifically for the purpose of preventing exposure of a user's hands to unhygienic viral and bacteriological contamination. The mechanism (100) is composed of three primary assembled components, a housing assembly (10), a cable / adjustment arm assembly (50), and a lift arm assembly (70) that work in unison to lift a toilet seat (102) via the application of pressure to a foot pedal (32). The housing assembly (10) is designed to be placed virtually anywhere within the proximity of the toilet (105), and the lift arm assembly (70) is designed to be installed onto the toilet (105) without any modifications whatsoever. This mechanism (100) was designed to be universally adaptable to most all toilet designs and was specifically devised with the intent to be so simplistic in design that manufacturing costs would be low enough to be commercially viable allowing the general public to utilize this product while maintaining a level of safety that will meet the most stringent of standards.

The present invention relates to a polypeptide with endolysin activity comprising an amino acid sequence according to SEQ ID No. 1 and fragments or derivatives thereof, or fusion proteins derived thereof. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide, fragment, derivative or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent. The present invention also relates to the use of said polypeptide, fragment, derivative or fusion protein for the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to a pharmaceutical composition comprising said polypeptide, fragment, derivative or fusion protein.

The invention relates to a preparation method and antibacterial application of plesiomonas shigelloides phage endolysin. The amino acid sequence of the plesiomonas shigelloides phage endolysin is Seq ID NO.2, and the plesiomonas shigelloides phage endolysin can be prepared into medicine inhibiting bacterial contamination or overgrowth and is applied as bactericide. According to the endolysin, amido bonds between saccharide and peptide of bacterial cell walls or linked bonds between amino acid residues in peptide are hydrolyzed to achieve lysis of host cells finally. The endolysin can act on a host specifically within a short action time and has a wider action spectrum than phage. The endolysin has the advantages of being efficient, specific, free of resistance and the like, and successful application of the endolysin has been reported in the food industry and the pharmaceutical industry at present.

The invention relates to a process for adsorbing traditional tableware by an environmentally friendly film and a preparation method thereof, which is characterized in that the environmentally friendly film is completely adsorbed on the tableware through electrostatic adsorption, which not only avoids the problem of food contamination by bacteria existing in the tableware during use, but also It solves the problem of cleaning tableware after use. Among them, the environmentally friendly film is made of PLA / PBAT mixed material. The PLA content in the environmentally friendly film is 60%, the PBAT content is 40%, and the film thickness is 0.015mm. It has good biocompatibility It can be composted and degraded under natural conditions, ensuring that the film will not pollute the environment after it is discarded; traditional tableware refers to ceramic tableware, metal tableware, melamine tableware, PLA injection tableware, etc. Conventional equipment is used, the process is simple, the operation is easy, and it is convenient for industrial promotion and application.

The present invention relates to a continuous method for producing products with molecules or macromolecules attached thereto and apparatus for carrying out this method. The method comprises the steps of: (a) placing the object on or in the proximity of a surface; (b) controlling the electrical potential of the surface with respect to its surroundings; (c) activating the object by exposing it to an electrical discharge; (d) contacting the object with the molecule or macromolecule to be attached. Such macromolecules include bacteriophage. Thus products of methods of the invention are for prevention and amelioration of bacterial contamination of the product of methods of the invention or materials in contact with said products.

The invention discloses a cell culture device. The cell culture device comprises a cell culture device body composed of a culture box, an oxygen storage tank, an observation mechanism and a debugging mechanism, wherein a culture cavity is formed in the culture box, a heating mechanism is fixedly connected to the bottom of the inner wall of the culture cavity, the top of the heating mechanism communicates with a water storage tank, an air injection plate and a culture frame are sequentially arranged at the top of the water storage tank, and the outer side of the air injection plate is fixedly connected with the inner wall of the culture cavity. The invention relates to the field of biotechnology. The cell culture device solves the problems that in the prior art, most of cell culture devices are in open culture, experimenters need to open the devices for observation, the interior is seriously polluted, the culture success rate is greatly reduced, each part cannot be specifically observed due to direct observation of a very small part, the observation record is inaccurate, meanwhile, the temperature of a culture dish cannot be well constant, and bacterial contamination is also easily caused by internal heating.

Bacterial contaminated surfaces may be disinfected or bacteria removed from a bacterial contaminated surface by applying onto the surface an anti-bactericidally effective amount of an ammonium compound represented by the formula R1COO−X+ wherein R1 is a C7 to C11 hydrocarbyl group, optionally substituted with one or more hydroxyl or C1-C5 hydrocarbyl groups and X is ammonium.

The invention provides a toothpick in irreversible color change after use and a method for preparing the toothpick in irreversible color change after use. The method includes the steps: A, processing bamboos or woods to obtain a toothpick body; B, soaking the toothpick body in petal extract with acid indicator functions; C, drying and sterilizing to obtain the toothpick in irreversible color change after use. By soaking of the toothpick in the petal extract, the toothpick has an irreversible color change under the action of intraoral secretion, and consequently cross infection is avoided, and unscrupulous businesses can be prevented from toothpick recovery. The toothpick prepared by the preparation method has an excellent antibacterial effect intrinsically, bacterial contamination of the unused toothpick is avoided, and cross infection is avoided due to irreversible color change of the toothpick.

The invention provides an antibacterial conductive composite film for water treatment as well as a preparation method and application of the antibacterial conductive composite film. The antibacterial conductive composite film comprises a base film and an antibacterial conductive layer arranged on the surface of the base film, the antibacterial conducting layer is made of a conducting material and a sterilizing material; the conductive material and the sterilizing material are doped in the antibacterial conductive layer, so that the problem of poor operation continuity easily generated when the base film is independently used is successfully solved, and the service life of the base film is effectively prolonged; and the conductive material and the sterilizing material of the antibacterial conductive layer can generate a synergistic effect, so that the antibacterial pollution resistance of the antibacterial conductive composite membrane is improved under the condition that the pure water membrane flux of the finally obtained antibacterial conductive composite membrane is not reduced, and the antibacterial conductive composite membrane has important research significance.

A compound of formula I: wherein R1 is a straight or branched alkyl group containing 8 to 16 carbon atoms at the 2- or 3-position of the morpholino ring, and R2 is a straight or branched alkyl group containing 2 to 10 carbon atoms, substituted with a hydroxy group except in the alpha-position, the sum of the carbon atoms in the groups R1 and R2 being at least 10 and preferably 10 to 20, is used to prevent biofilm formation on a surface. The compounds are particularly useful as coatings or treatments for medical devices, including stents, catheters and wire guides.

The present invention relates to compositions and methods for protection against bacterial contamination. The invention provides bactericidal yeast expressing bacteriocin proteins and methods of using the bactericidal yeast.

The invention relates to the field of biotechnology renewable energy, in particular to a method for producing endoglucanase by fermenting roughage substrates with a natural symbiotic mixed culture YakQH5, wherein the mixed culture YakQH5 is composed of anaerobic fungi (Neocallimastix frontalis) Composed of Methanobrevibacter gottschalkii, it can degrade different roughages and produce endoglucanase with high enzymatic activity, among which the activity of endoglucanase produced by degrading wheat hulls can reach 1170mU, which has achieved remarkable results Adding compound antibiotics in the fermentation process can also prevent the mixed culture YakQH5 from being polluted by bacteria during the fermentation process, and further improve the efficiency of anaerobic fermentation. The mixed culture adopted in the present invention can be preserved in vitro for survival and passage, which is convenient for popularization and provides convenience for production.

The invention relates to a method for preparing composite hydrophobic nano powder-coated liquid marbles, which belongs to the technical field of coating materials. The invention uses nano-zinc oxide powder, nano-titanium dioxide powder and nano-alumina powder as raw materials to prepare a composite hydrophobic nano-powder that wraps liquid, and can effectively reduce the liquid in the marble by wrapping the hydrophobic nano-zinc oxide powder. The risk of harmful bacterial contamination, thereby improving the safety of liquid marbles, nano-titanium dioxide has a strong UV shielding effect, and is effective against Bacillus subtilis var. niger, Pseudomonas aeruginosa, E. The extinction rate reaches 98~99%, which can effectively improve the medical use and safety of liquid marbles. Nano-alumina is in the state of white fluffy powder, which has the advantages of large specific surface area, high porosity, strong adsorption and good formability. It can effectively adsorb on the surface of the liquid, and effectively adsorb and bond other particles on the surface of the liquid, thus forming a stable liquid marble.

The invention discloses a sewage and garbage storage device. The sewage and garbage storage device comprises a storage chamber 1 and a drainage chamber 2, wherein the storage chamber 1 is arranged below the drainage chamber 2, and compaction devices 11 are arranged in the storage chamber 1 and are arranged on the two sides of the storage chamber 1 correspondingly; a perforated filter plate is arranged at the lower part of the storage chamber 1; a drainage pipe is arranged on one side of the drainage chamber 2 and is connected with a water treatment tank; and the storage chamber 1 is also provided with a garbage clearing and transporting door 12. According to the sewage and garbage storage device, leachate in garbage can be discharged in time, and the situation that the garbage breeds more bacteria in a high humidity environment, and the surrounding air environment is polluted is reduced.

The invention discloses a method for evaluating the pathogenicity of grape canker bacteria on grapes. The method is: take the bacterial strains cultured on the PDA plate, take the bacteria cake and place it in the improved Fries liquid medium for static culture for 14 days, centrifuge to collect the supernatant, filter with a pinhole filter to remove mycelia, spores and bacterial contamination , that is, the preparation of the crude toxin stock solution is completed. Select new young leaves of annual green branches, after surface disinfection, take out a leaf disc with a diameter of 10mm, absorb 10 μL of crude toxin stock solution and inoculate it in the central part of the leaf disc, measure the lesion area after 3 days, and evaluate the potency of grape canker sore crude toxin stock solution pathogenicity. The invention has the advantages of simple operation, low cost and good repeatability, and provides technical support for the research on the effect of the mycotoxin in the pathogenic process of grape canker bacterium and the research on the selection of grape varieties resistant to grape canker.

The invention discloses a mask outer packaging bag capable of invalidating regularly and a production method thereof, and relates to the field of packaging bags, a biodegradable film with good biodegradability is prepared firstly, then the biodegradable film is coated with an antibacterial and hydrophobic coating with good antibacterial and bacteriostatic performance and water-repellent and stain-resistant performance, and a timed invalidating film is obtained; the mask outer packaging bag capable of invalidating regularly is obtained by cutting the timed invalidating film into a packaging bag, the mask outer packaging bag capable of invalidating regularly has good antibacterial and hydrophobic performance, the antibacterial performance can ensure that the safety is good and bacterial pollution is avoided when the mask is loaded, and the hydrophobic performance can ensure that the packaging bag is not easy to stick dust and rot when meeting water, and the sanitation degree of the mask is further ensured; after the mask outer packaging bag capable of invalidating regularly is discarded, the biodegradable film is exposed after the mask outer packaging bag is crushed, and the mask outer packaging bag can be quickly degraded through good biodegradability.