Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages
A development stage, chestnut technology, applied in the direction of biochemical equipment and methods, botany equipment and methods, microbial measurement/inspection, etc., can solve the problems of poor firmness, low yield per mu, idle land, etc., and achieve a good society and economy benefit effect
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Embodiment 1
[0068] Example 1. Identification and screening of shade-tolerant millet germplasm resources.
[0069] From 2015 to 2016 in Qianxi, the method of planting the test materials in the field and under the 10-year-old chestnut tree at the same time was used to measure the performance of plant height, fruitiness, photosynthetic efficiency and yield of 153 germplasm resources, and conduct a comparative analysis. Nine new cultivars / germplasm resources with good shade tolerance were screened out from "Jigu 32, 56229, 3495, 51950, 56950, JK4-95, HK229, 56A, 51A". These materials were planted under 10-year-old chestnut trees. Compared with the field, the photosynthetic rate did not decrease slightly, and the plant height and fruiting were almost unchanged. However, other materials showed a large decrease in photosynthetic rate, and due to lack of sunlight, the plant height increased significantly, and the fruitiness decreased significantly.
[0070]Table 1 The shade-tolerant identificati...
Embodiment 2
[0073] Example 2. Mining of shade-tolerant genes and development of markers.
[0074] Using the shade-tolerant germplasm resources Jigu 32 and JK4-95 screened in Example 1 and the non-shade-tolerant germplasm resources Jigu 31 and FK-1 as materials, the leaves of the three-leaf sword were selected at the booting stage in mid-July 2016, and passed RNA-Seq to obtain their transcriptome information; through the differential expression analysis of the genes of the two types of materials, the differentially expressed genes were obtained; through bioinformatics analysis and evolution analysis, their functions were further speculated; some of the genes with prominent functions were cloned; Through gene sequencing, ascertain its gene sequence and develop a series of shade tolerance markers.
Embodiment 3
[0075] Embodiment 3, creation of shade-tolerant breeding material (sterile line, restorer line)
[0076] Use the shade-tolerant resources screened in Example 1 as materials to prepare a series of hybrid combinations; use the series of shade-tolerant molecular markers developed in Example 2 to create shade-tolerant sterile lines and restorer lines through molecular marker-assisted selection.
[0077] 1) Creation of shade-tolerant CMS lines: In the summer of 2016, 56A and 51A were used as female parents, and KM200 and KM117 were used as male parents to prepare the first generation of hybrids; in winter 2017, Datong 29 and 206083 were used as female parents, The first generation of hybrids formulated in summer was used as the male parent, and the three-cross combination was continued to be prepared. Then, after four consecutive generations of south-to-north breeding, and based on molecular markers, the shade-tolerant offspring were selected to breed new CMS lines DZ40A and DZ386A....
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