Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages

A development stage, chestnut technology, applied in the direction of biochemical equipment and methods, botany equipment and methods, microbial measurement/inspection, etc., can solve the problems of poor firmness, low yield per mu, idle land, etc., and achieve a good society and economy benefit effect

Active Publication Date: 2021-06-08
QIANXI COUNTY JINDI CHESTNUT FOOD CO LTD
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AI Technical Summary

Problems solved by technology

[0003] The chestnut planting areas are all mountainous counties, and the shortage of land resources is a common feature of these areas. Due to the lack of suitable interplanting crops, a large amount of land under chestnut trees has been abandoned all year round, overgrown with weeds, and serious pests and diseases, especially the damage of red spiders. , not only caused the production of chestnut and other fruits to decrease, but also the rate of bad fruit increased year by year, and the quality decreased year by year, which seriously affected the income of farmers and had a great impact on brands such as Jingdong chestnut; it is urgent to grow normally under the tree and reduce red Three-dimensional planting of new varieties of crops that are harmed by pests and diseases such as spiders to improve the quality of fruits and under-tree crops, realize efficient use of the land under the trees, solve the problems of land shortage and difficult development of agriculture-related industries in these areas, and improve the quality of farmers. income, and speed up the progress of farmers towards a well-off life
[0004] In order to solve the above problems, farmers voluntarily interplant soybeans, peanuts, corn, sorghum and other crops under the trees. However, since chestnut orchards are mostly located in shallow hilly areas, the soil is barren, dry and rainless, and soybeans and peanuts need to be planted on soil with better soil quality. In addition, there is a lack of shade-tolerant soybean and peanut varieties, and the growth vigor of soybean and peanut under chestnut trees is relatively poor. Not only is the yield low, and the per unit yield is less than 75kg / mu, but also due to the long growth period of soybean and peanut, it affects the picking of walnuts. It is difficult to achieve the coupling of key growth stages; while corn and sorghum plants are tall, and due to the lack of shade-tolerant varieties, interplanting with chestnut trees, the stems are thin and weak, not resistant to lodging, the yield is low and unstable, and there is also a long growth period, which affects Walnut picking, it is difficult to achieve the coupling of key growth stages
However, not all millet varieties are suitable for three-dimensional interplanting with chestnuts and other fruit trees. The test results show that most varieties and chestnuts are interplanted due to lack of light, showing that the plants become taller (10-20cm higher than the field), serious diseases, and excessive leaves. They turn yellow and dry early, have poor firmness, low yield per mu, less than 120 kg; at the same time, we have also screened some breeding materials, which are interplanted with chestnut. Compared with the field, the plant height hardly changes, and the green branches and green leaves are mature, and 10 The three-dimensional interplanting of chestnuts aged 15 years old has the same yield as that of the field, and the three-dimensional interplanting of 15-year-old chestnuts has a slightly lower yield than that of the field. However, these materials have problems such as low yields and deviations in disease resistance. Therefore, it is urgent to breed new millet with good shade tolerance. Varieties / hybrids provide technical support in terms of varieties for carrying out three-dimensional interplanting of chestnut valleys and solving the problems of a large amount of idle land under the trees in chestnut orchards, waste, and serious pests and weeds, so as to improve the quality of chestnuts, increase the yield of millet, and improve the planting efficiency of farmers. It also provides a feasible way to expand the planting area of ​​millet and restore and develop the millet industry
[0006] In addition, the three-dimensional intercropping of chestnut valley needs to plant two crops of chestnut and millet on the same land at the same time. Only when the key stages of fertilizer and water requirements of the two crops overlap, that is, the key development stages are basically the same, can fertilizer, water, and pests and diseases be simultaneously controlled. Field management such as prevention and control can form complementary or mutual promotion in some aspects of chestnut and millet, so as to achieve mutual benefit and symbiosis. Otherwise, various managements are inconsistent, not only management is labor-intensive and time-consuming, but also there are management measures that are beneficial to one plant. However, the possibility of another plant is suppressed, and the effect of the three-dimensional planting of chestnut valley is difficult to be reflected. Therefore, it is urgent to invent a method that can ensure that the key management measures of chestnut and millet under the three-dimensional interplanting of chestnut valley are consistent.

Method used

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  • Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages
  • Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages
  • Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1. Identification and screening of shade-tolerant millet germplasm resources.

[0069] From 2015 to 2016 in Qianxi, the method of planting the test materials in the field and under the 10-year-old chestnut tree at the same time was used to measure the performance of plant height, fruitiness, photosynthetic efficiency and yield of 153 germplasm resources, and conduct a comparative analysis. Nine new cultivars / germplasm resources with good shade tolerance were screened out from "Jigu 32, 56229, 3495, 51950, 56950, JK4-95, HK229, 56A, 51A". These materials were planted under 10-year-old chestnut trees. Compared with the field, the photosynthetic rate did not decrease slightly, and the plant height and fruiting were almost unchanged. However, other materials showed a large decrease in photosynthetic rate, and due to lack of sunlight, the plant height increased significantly, and the fruitiness decreased significantly.

[0070]Table 1 The shade-tolerant identificati...

Embodiment 2

[0073] Example 2. Mining of shade-tolerant genes and development of markers.

[0074] Using the shade-tolerant germplasm resources Jigu 32 and JK4-95 screened in Example 1 and the non-shade-tolerant germplasm resources Jigu 31 and FK-1 as materials, the leaves of the three-leaf sword were selected at the booting stage in mid-July 2016, and passed RNA-Seq to obtain their transcriptome information; through the differential expression analysis of the genes of the two types of materials, the differentially expressed genes were obtained; through bioinformatics analysis and evolution analysis, their functions were further speculated; some of the genes with prominent functions were cloned; Through gene sequencing, ascertain its gene sequence and develop a series of shade tolerance markers.

Embodiment 3

[0075] Embodiment 3, creation of shade-tolerant breeding material (sterile line, restorer line)

[0076] Use the shade-tolerant resources screened in Example 1 as materials to prepare a series of hybrid combinations; use the series of shade-tolerant molecular markers developed in Example 2 to create shade-tolerant sterile lines and restorer lines through molecular marker-assisted selection.

[0077] 1) Creation of shade-tolerant CMS lines: In the summer of 2016, 56A and 51A were used as female parents, and KM200 and KM117 were used as male parents to prepare the first generation of hybrids; in winter 2017, Datong 29 and 206083 were used as female parents, The first generation of hybrids formulated in summer was used as the male parent, and the three-cross combination was continued to be prepared. Then, after four consecutive generations of south-to-north breeding, and based on molecular markers, the shade-tolerant offspring were selected to breed new CMS lines DZ40A and DZ386A....

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Abstract

The invention discloses a Castanea mollissima-shade-tolerant millet three-dimensional interplanting method with synergistic and consistent key growth stages. The method comprises the following steps: a comprehensive database of the key growth stages of Castanea mollissima and millet is established, and a database of growth processes of Castanea mollissima and millet is compared and analyzed, so that the key growth stages of Castanea mollissima and millet coincide; then, according to Castanea mollissima and millet fertilizer and water demand rules and pest and disease damage occurrence rules, Castanea mollissima and millet mutualism fertilizer and water management measures are determined, Castanea mollissima-millet common high yield and pest and disease damage control synergistic complementary effects are achieved, unit land planting benefits are greatly improved, and pest and disease damage of Castanea mollissima mainly including red spiders is reduced. According to the method, mutualism of the Castanea mollissima and the millet is realized, so that the two plants of the Castanea mollissima and the millet can exert the maximum yield-increasing and value-increasing potential.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for breeding new shade-tolerant millet varieties, coupling key growth stages of chestnuts and millet, and matching key management measures under the three-dimensional interplanting planting mode of chestnuts and valleys. Background technique [0002] my country's chestnut planting area is about 10 million mu, with a total output of about 1.5 million tons. Among the 31 provinces and autonomous regions in the country, 24 provinces and cities have planted chestnuts. Among them, there are 20 counties and cities with an annual output of more than 1 million kilograms, including Beijing, Tianjin and Hebei. There are 9 regions, accounting for 45% of the country. The chestnut industry has become the leading characteristic industry in these regions and occupies a pivotal position in the county's economic development. [0003] The chestnut planting areas are all mountainou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G22/20A01G13/00A01H1/02A01H1/04C12Q1/6895
CPCA01G17/005A01G22/20A01G13/00A01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/158Y02A40/28
Inventor 刘丹崔燕娇李素英李承宗赵子龙刘正理
Owner QIANXI COUNTY JINDI CHESTNUT FOOD CO LTD
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