Microfluidic paper chip, preparation method thereof, microfluidic paper chip detection system and application

Abstract

The invention provides a microfluidic paper chip, which comprises a sample introduction area and a detection area, wherein the sample introduction area and the detection area are hydrophilic areas, and the periphery of the sample introduction area and the detection area is a hydrophobic area; the sample introduction area is positioned on one side of the paper chip, and the detection area is positioned on the other side; and the sample introduction area can penetrate through the paper base to penetrate through the other side of the paper chip to form a filtrate area, and the filtrate area enables filtrate to flow to the detection area in a contact mode or a non-contact mode. The invention further discloses a method for preparing the microfluidic paper chip and a detection system based on the microfluidic paper chip, and the system is applied to detection of components of food, vegetables, traditional Chinese medicinal materials and blood samples. The paper chip disclosed by the invention is simple and convenient to use, low in cost and more suitable for detecting substances with relatively small sample quantity. When a sample injection solution is ionized and sprayed, a signal entering a mass spectrometer is stronger, and the detection precision is higher. During detection operation, pollution can be prevented, the accuracy of detection data is ensured, and the mass spectrometer is prevented from being damaged.

Description

technical field [0001] The invention relates to a microfluidic paper chip, a preparation method thereof, a detection system and application of the microfluidic paper chip, and belongs to the technical field of chemical composition testing. Background technique [0002] The microfluidic paper chip is to establish a "hydrophobic barrier" and "hydrophilic channel" in a specific area of ​​the filter paper, and then construct a paper-based microfluidic chip with a certain network structure of hydrophilic and hydrophobic channels. The microfluidic paper chip was proposed by the team of Professor Whitesides of Harvard University in the United States in 2007, and has become a promising development direction in microfluidic chips. Compared with traditional microfluidic chips based on silicon, glass and plastic, microfluidic paper chips have many advantages such as wide source of raw materials, low price, easy processing, good compatibility with biological samples, and degradability. ...

AI Technical Summary

Problems solved by technology

However, in the process of stacking or folding, precise alignment and tight fit between layers are required, which is cumbersome to operate, and the fluid is easily lost due to insufficient tight fit between layers during the layer-by-layer flow, and in the stacking process Need to use double-sided tape, it is easy to produce non-specific adsorption during the analysis and determination process, pollute the sample, and have a certain impact on the accuracy of the analysis results

Images