Beta-amyloid protein targeting fluorescent probe, preparation and application of beta-amyloid protein targeting fluorescent probe in Alzheimer's disease
A technology for Alzheimer's disease and amyloid protein, applied in the field of biomedicine, can solve the problems of difficult penetration and low sensitivity of iodinated probes, and achieve the effect of long emission wavelength, good response and enhanced fluorescence signal
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Embodiment 1
[0109] The preparation of embodiment 1 fluorescent probe compound (the synthetic route of fluorescent probe sees figure 1 , see the chemical structure figure 2 )
[0110] 1. The synthetic steps of compound 1;
[0111] First prepare the hydrochloric acid solution (HCl:H 2 O=1:1, v / v) 250ml, stir evenly, add 5.8g (42.6mmol) of N,N-dimethyl-1,4-p-phenylenediamine, after stirring evenly, add 7ml of crotonaldehyde, mix well Then, 30 ml of toluene was added to the reaction liquid, and the reaction was refluxed for 7 h under the protection of argon at 115° C. Thin-layer chromatography confirmed that the reaction was complete, the reaction was stopped, and the reaction was allowed to stand at room temperature. First remove the upper toluene solution, then adjust the pH to neutral with NaOH saturated solution under ice-water bath conditions, then add an equal volume of DCM to extract at least three times, and finally separate and purify by silica gel column chromatography (silica ...
Embodiment 2
[0321] (1) Preparation of Aβ aggregates;
[0322] The purchased Aβ1-42 aggregate protein (purchased from Qiangyao Biotechnology Co., Ltd.) was dissolved in 1% (v / v) ammonia water at a concentration of 100 μM, and continuously incubated at 37°C for one week to obtain Aβ1-42 aggregates.
[0323] (2) Determination of fluorescence spectrum
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