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Nucleic acid detection pretreatment instrument

A nucleic acid and sample technology, applied in biomass post-processing, biological material pre-processing, biochemical instruments, etc., can solve the problems of complex operation in the pre-processing process, increasing the risk of transmission, and long overall processing time.

Pending Publication Date: 2021-06-08
苏州博捷特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The entire pretreatment process is complicated and requires professional technicians; (2) Even if a nucleic acid extractor is used, it only replaces the process of lysing, washing and elution. Both opening and closing of the cap and capping the PCR tube need to be done manually; (3) The overall processing time is long, usually 2-3 hours, even if a nucleic acid extraction instrument is used, it will take 1-2 hours; (4) If the original sample is infected Sexual samples, the process of uncapping and sampling increases the risk of transmission, which is not good for operators

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0043] Such as Figure 1-5 As shown, this product is a pre-processing instrument for nucleic acid detection, including sample tube 11, 96-well plate 8 and eight-tube tubes pre-installed in the processing instrument, and also includes: a cap screwing device installed in the processing instrument, located in Next to the sample tube 11, it is used to clamp the sample tube 11 and loosen or tighten the sample tube cover 111; the pipette 3 is located on the top of the processing instrument and is used to carry and transfer the sample; the magnetic rod displacement device is located at 96 Next to the orifice plate, it is used to absorb sample nucleic acid and magnetic beads, and realize the extraction operation of the samples in the above-mentioned 96-well plate 8; the eight-tube receiving device is located at the bottom of the processor, and is used to receive the extracted samples to be tested.

[0044] More specifically, the cap screwing device of this embodiment includes a clamp ...

Embodiment 2

[0080] Based on the product of Embodiment 1, this embodiment can adopt more optional solutions including:

[0081] (1) The clamp can cooperate with the pressure sensor to realize the clamping of sample tubes of different dimensions.

[0082] (2) The rotating head can cooperate with the pressure sensor to realize the tightness of different sample tube caps.

[0083] (3) The tip box can be in the shape of a whole box, a shelf, or a laminated shelf.

[0084] (4) The tip box can be divided into different specifications of tips, such as A, B, or only one specification.

[0085] (5) The pipette can be single or two different, so as to match different suction heads to achieve the suction of different volumes of liquid.

[0086] (6) The hole shape of the 96-well plate can be square or round; the bottom can be round or conical; the bottom of the magnetic bar sleeve can be round, conical or flat.

[0087] (7) The specification of the eight-tube tube can be 0.1mL or 0.2mL; the edge of...

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Abstract

The invention relates to a nucleic acid detection pretreatment instrument. The nucleic acid detection pretreatment instrument is mainly used for extracting nucleic acid in a biological sample and belongs to the technical field of nucleic acid detection. The treatment instrument comprises a sample tube, a 96-well plate and an eight-connection tube which are arranged in the treatment instrument in advance; the treatment instrument further comprises a cap screwing device, a pipettor, a magnetic rod displacement device and an eight-connection tube receiving device which are arranged in the treatment instrument; the cap screwing device is used for clamping the sample tube and loosening or tightening a tube cap of the sample tube; the pipettor is used for carrying and transferring a sample; the magnetic rod displacement device is used for adsorbing nucleic acid and magnetic beads of a sample and extracting the sample in the 96-well plate; and the eight-connection tube receiving device is used for receiving the extracted sample to be detected. All manual operations in the prior art can be replaced by a full-automatic instrument; and the requirement on the professional degree of an operator is greatly reduced through a preset program and one-button operation.

Description

technical field [0001] The invention relates to a nucleic acid detection preprocessing instrument, which is mainly used for the extraction of nucleic acid in biological samples and belongs to the technical field of nucleic acid detection. Background technique [0002] In the prior art, before performing nucleic acid detection, it is necessary to perform nucleic acid extraction on original samples (such as throat swabs, alveolar lavage fluid, blood, etc.). At present, the magnetic bead method is more common in clinical application, that is, the original sample is sucked out of the original storage tube, added to the lysate, oscillated and mixed, and the lysate is used to destroy the target cell structure in the original sample, so that the cells in the cell The nucleic acid is released; then add magnetic beads treated with surface groups to absorb nucleic acid, then use a magnet to absorb the magnetic beads with nucleic acid attached, and transfer to washing solution 1; remov...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/38C12M1/34C12M1/26C12M1/24C12M1/12C12M1/04C12M1/00
CPCC12N15/1013
Inventor 刘杰胡彬申富树
Owner 苏州博捷特生物科技有限公司
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