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Method for producing citric acid by fermenting aspergillus niger

A technology of Aspergillus niger fermentation and citric acid, which is applied in the field of microbial fermentation, can solve the problems of long production cycle, unstable quality, and large production volume, and achieve the effects of reducing production volume, improving stability, and shortening the production cycle

Active Publication Date: 2021-06-08
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Aspergillus niger expansion in order to overcome the problems of large production quantity of Aspergillus niger seeds in the prior art, long production cycle and unstable quality. Cultivate and use the method for citric acid fermentation and Aspergillus niger, realize the purpose of reducing the production amount and production cycle of Aspergillus niger seeds, and improving the quality stability of Aspergillus niger seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1) Sterilize the first-level seed medium at 121°C for 20 minutes, inoculate the Aspergillus niger spore powder on the first-level seed medium, and the inoculation amount of Aspergillus niger is 5×10 6 cfu, cultured at 33°C and 400rpm for 24h to obtain a first-grade seed solution containing Aspergillus niger balls (average diameter of 180 μm);

[0066] 2) Add glass microspheres with a diameter of 4 mm to the first-grade seed solution containing Aspergillus niger balls, and use a shaker for 12 minutes at a temperature of 33°C and 380 rpm to obtain hyphae with an average length of 30 μm; For 100mL of the first-grade seed solution containing Aspergillus niger balls, the amount of glass microspheres is 100;

[0067] 3) Inoculate the first-level seed liquid after shaking treatment into the second-level seed medium, and the inoculum amount of mycelium is 1.3×10 6 The root mycelia is inoculated, and the stirring is started, and at a culture temperature of 36° C., and a dissolv...

Embodiment 2

[0071] 1) Sterilize the primary seed medium at 121°C for 20 minutes, inoculate the Aspergillus niger spore powder on the primary seed medium, and the inoculum amount of Aspergillus niger is 7×10 relative to 1 mL of the primary seed medium. 6 cfu, cultivated at 35°C for 20h at 500rpm to obtain a primary seed solution containing Aspergillus niger balls (average diameter of 150 μm);

[0072] 2) Add glass microspheres with a diameter of 2 mm to the primary seed liquid containing Aspergillus niger balls, and use a shaker for 10 minutes at a temperature of 35°C and 350 rpm to obtain hyphae with an average length of 35 μm ; Relative to 100mL of the first-grade seed solution containing Aspergillus niger balls, the consumption of glass microspheres is 150.

[0073] 3) Inoculate the first-level seed liquid after shaking treatment in the second-level seed medium, and relative to 1mL of the second-level seed medium, the inoculum amount of mycelium is 1.5×10 6 root mycelia / ml to inoculate...

Embodiment 3

[0077] 1) Sterilize the first-level seed medium at 121°C for 20 minutes, inoculate the Aspergillus niger spore powder on the first-level seed medium, and the inoculum amount of Aspergillus niger is 8×10 relative to 1 mL of the first-level seed medium. 6 cfu, cultivated at 30°C for 30h at 300rpm to obtain a primary seed liquid containing Aspergillus niger balls (average diameter of 200 μm);

[0078] 2) Add glass microspheres with a diameter of 5 mm to the first-grade seed solution containing Aspergillus niger balls, and use a shaker for 15 minutes at a temperature of 30°C and 400 rpm to obtain hyphae with an average diameter of 25 μm ; Relative to 100mL of the first-grade seed liquid that contains Aspergillus niger balls, the consumption of glass microspheres is 80;

[0079] 3) Inoculate the first-level seed liquid after shaking treatment in the second-level seed medium, and the inoculum amount of mycelium is 1×10 6 root mycelia / ml for inoculation, start stirring, and cultivat...

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Abstract

The invention relates to the field of microbial fermentation, and discloses a method for producing citric acid by fermenting aspergillus niger. The method comprises the following steps: inoculating the aspergillus niger in a primary seed culture medium for culture to prepare a primary seed solution containing aspergillus niger pellets; dispersing the aspergillus niger pellets in the primary seed solution, so that the aspergillus niger pellets form hyphae with the average length of 20-40 microns; a secondary seed culture medium is inoculated with the primary seed solution subjected to dispersion treatment for culture, a secondary seed solution containing aspergillus niger pellets is prepared, and the average diameter of the aspergillus niger pellets in the secondary seed solution is 30-50 microns; and the secondary seed solution containing the aspergillus niger pellets are inoculated to a fermentation medium for citric acid fermentation. According to the method, the preparation amount of the aspergillus niger seeds is reduced, the preparation period of the aspergillus niger seeds is shortened, the quality stability of the aspergillus niger seeds is improved, and when the aspergillus niger seeds are used for fermenting citric acid, the yield and the fermentation conversion rate are high.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing citric acid by fermentation of Aspergillus niger. Background technique [0002] The citric acid fermentation adopts the secondary fermentation method of Aspergillus niger, that is, the mature seeds are cultivated first, and then the mature seed liquid is transferred to ferment citric acid. The seed culture process of Aspergillus niger first needs to cultivate mature spores of Aspergillus niger, and then transfer the spores to obtain mature seeds. In industrial production, a large number of mature spores need to be prepared, but at present most of the production adopts solid-state culture in triangular flasks, the preparation process is cumbersome and the cycle is long, and the preparation cycle of a batch of mature spores is at least 10 days or more. Due to the large amount of Aspergillus niger spores produced and the long culture period, the quality ...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12N1/14C12R1/685
CPCC12P7/48C12N1/14
Inventor 熊结青刘梦涵杨儒文佟毅李义朱光耀卜庆阳
Owner COFCO BIOTECHNOLOGY CO LTD
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