Rapid detection method and application of phomopsis nicotianae

A technology of P. pseudomycetes and a detection method, which is applied in the biological field and can solve problems such as inconvenience to distinguish diseases with similar symptoms.
CN112921116APending Publication Date: 2021-06-08HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2021-06-08

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Abstract

The invention provides a rapid detection method for phomopsis nicotianae and application of the rapid detection method. The rapid detection method for the phomopsis nicotianae comprises the following steps of extracting genome DNA (Deoxyribose Nucleic Acid); designing a specific primer; detecting the specific primer; and detecting the sensitivity of the specific primer. According to the rapid detection method for the phomopsis nicotianae, the molecular information of the phomopsis nicotianae fungi can be rapidly, specifically and sensitively detected, and only one PCR reaction is carried out on genomic DNA of pathogenic bacteria as low as 10 pg; the method overcomes the defect that in the prior art, the time needed for morphological analysis and confirmation is too long only through strain isolated culture, disease control delay caused by the fact that symptoms are not easy to distinguish is avoided, meanwhile, pathogenic bacteria can be accurately detected in time according to the existence of trace pathogenic bacteria, disease control and early warning are facilitated, and meanwhile, a scientific basis is provided for further research of phomopsis.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and in particular relates to a rapid detection method and application of Phomopsis tobacco. Background technique

[0002] In recent years, due to a series of problems such as the expansion of tobacco cultivation area, continuous crop rotation and single variety, tobacco diseases have become more frequent or more serious. Tobacco diseases have always been an important factor restricting tobacco production. The variety of pathogens is complex, causing Fusarium wilt, black shank, root black rot, and Phomopsis stem blight caused by Phomopsis spp., causing serious harm.

[0003] Traditional fungal identification methods have always been mainly based on morphology, by observing the morphological characteristics of the colony after cultivation, observing the structural characteristics with a microscope, whether spores are produced, physiological and biochemical characteristics, and so on. However, ...

Claims

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